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Bioremediation of petroleum sludge was conducted by using land-farming method in micro scale and by applying an indigenous bacteria Bacillus megaterium. The samples were from PT. Pertamina Musi Banyuasin district of South Sumatra. The research aim was to evaluate the performance of the bacteria in degrading petroleum sludge. The rate of the biodegradation process was determined by using differential method and the data analyses show that the reaction order is 0.74. Then, the rate of biodegradation constant was determined by using an integral method assuming that the biodegradation process was a first reaction order. From the calculation, it was revealed that the biodegradation reaction constant was 0.0204/day. The bioremediation-kinetics model is y = -0.0204X + 2.0365, and by using this model the bioremediation process could be ended after 99.83 days. The qualitative analysis was carried out by using GC-MS to investigate the components of compounds changed during the bioremediation process. The results show that the B. megaterium could degrade 99.32% of alkane compounds.

2011

YUDONO, BAMBANG | SAID, MUHAMMAD | SABARUDDIN, . | NAPOLEON, ADIPATI | UTAMI, MARYATI BUDI


Bibliographic information
Publisher
Bogor Agricultural University, Indonesia
Other Subjects
">days; The rate of biodegradation constant was determined by using an integral method assuming that the biodegradation process was a first reaction order. from the calculation; ">. t; 14.15pt; It was revealed that the biodegradation reaction constant was 0.0204/day. the bioremediation-kinetics model is y = -0.0204x 2.0365; Text-indent; 9pt; ">bioremediation of petroleum sludge was conducted by using land-farming method in micro scale and by applying an indigenous bacteria bacillus megaterium. the samples were from pt. pertamina musi banyuasin district of south sumatra. the research aim was to evaluate the performance of the bacteria in degrading petroleum sludge. the rate of the biodegradation process was determined by using differential method and the data analyses show that the reaction order is 0.74. then; 5pt; And by using this model the bioremediation process could be ended after 99.83; ">he qualitative analysis was carried out by using gc-ms to investigate the components of compounds changed during the bioremediation process. the results show that the b. megaterium could degrade 99.32% of alkane; Justify; ">; ">compounds.
Language
English
Format
application/pdf
ISSN
2086-4094, 1978-3019
Type
Info:eu-Repo/semantics/article; Info:eu-Repo/semantics/publishedversion; Peer-Reviewed Article
Source
HAYATI Journal of Biosciences; Vol. 17 No. 4 (2010): December 2010; 155, 2086-4094, 1978-3019

2025-06-17
Dublin Core
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DOI https://journal.ipb.ac.id/index.php/hayati/article/view/%20%3Cp%20class%3D%22MsoNormal%22%20style%3D%22text-align%3Ajustify%3Btext-indent%3A14.15pt%3B%22%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3EBioremediation%20of%20petroleum%20sludge%20was%20conducted%20by%20using%20land-farming%20method%20in%20micro%20scale%20and%20by%20applying%20an%20indigenous%20bacteria%20%3Cem%3EBacillus%20megaterium%3C%2Fem%3E.%20The%20samples%20were%20from%20PT.%20Pertamina%20Musi%20Banyuasin%20district%20of%20South%20Sumatra.%20The%20research%20aim%20was%20to%20evaluate%20the%20performance%20of%20the%20bacteria%20in%20degrading%20petroleum%20sludge.%20The%20rate%20of%20the%20biodegradation%20process%20was%20determined%20by%20using%20differential%20method%20and%20the%20data%20analyses%20show%20that%20the%20reaction%20order%20is%200.74.%20Then%2C%20the%20rate%20of%20biodegradation%20constant%20was%20determined%20by%20using%20an%20integral%20method%20assuming%20that%20the%20biodegradation%20process%20was%20a%20first%20reaction%20order.%20From%20the%20calculation%2C%20it%20was%20revealed%20that%20the%20biodegradation%20reaction%20constant%20was%200.0204%2Fday.%20The%20bioremediation-kinetics%20model%20is%20y%20%3D%20-0.0204X%20%2B%202.0365%2C%20and%20by%20using%20this%20model%20the%20bioremediation%20process%20could%20be%20ended%20after%2099.83%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3E%20%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3Edays%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3E.%20T%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3Ehe%20qualitative%20analysis%20was%20carried%20out%20by%20using%20GC-MS%20to%20investigate%20the%20components%20of%20compounds%20changed%20during%20the%20bioremediation%20process.%20The%20results%20show%20that%20the%20%3Cem%3EB.%20megaterium%20%3C%2Fem%3Ecould%20degrade%2099.32%25%20of%20alkane%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A5pt%3B%22%3E%20%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3Ecompounds.%3C%2Fspan%3E%3C%2Fstrong%3E%3C%2Fp%3E https://journal.ipb.ac.id/index.php/hayati/article/view/%20%3Cp%20class%3D%22MsoNormal%22%20style%3D%22text-align%3Ajustify%3Btext-indent%3A14.15pt%3B%22%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3EBioremediation%20of%20petroleum%20sludge%20was%20conducted%20by%20using%20land-farming%20method%20in%20micro%20scale%20and%20by%20applying%20an%20indigenous%20bacteria%20%3Cem%3EBacillus%20megaterium%3C%2Fem%3E.%20The%20samples%20were%20from%20PT.%20Pertamina%20Musi%20Banyuasin%20district%20of%20South%20Sumatra.%20The%20research%20aim%20was%20to%20evaluate%20the%20performance%20of%20the%20bacteria%20in%20degrading%20petroleum%20sludge.%20The%20rate%20of%20the%20biodegradation%20process%20was%20determined%20by%20using%20differential%20method%20and%20the%20data%20analyses%20show%20that%20the%20reaction%20order%20is%200.74.%20Then%2C%20the%20rate%20of%20biodegradation%20constant%20was%20determined%20by%20using%20an%20integral%20method%20assuming%20that%20the%20biodegradation%20process%20was%20a%20first%20reaction%20order.%20From%20the%20calculation%2C%20it%20was%20revealed%20that%20the%20biodegradation%20reaction%20constant%20was%200.0204%2Fday.%20The%20bioremediation-kinetics%20model%20is%20y%20%3D%20-0.0204X%20%2B%202.0365%2C%20and%20by%20using%20this%20model%20the%20bioremediation%20process%20could%20be%20ended%20after%2099.83%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3E%20%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3Edays%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3E.%20T%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3Ehe%20qualitative%20analysis%20was%20carried%20out%20by%20using%20GC-MS%20to%20investigate%20the%20components%20of%20compounds%20changed%20during%20the%20bioremediation%20process.%20The%20results%20show%20that%20the%20%3Cem%3EB.%20megaterium%20%3C%2Fem%3Ecould%20degrade%2099.32%25%20of%20alkane%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A5pt%3B%22%3E%20%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3Ecompounds.%3C%2Fspan%3E%3C%2Fstrong%3E%3C%2Fp%3E/1222
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