Maize nutrition quality and plant tissue analysis laboratory: laboratory protocols 2008
2009
Galicia, L. | Nurit, E. | Rosales Nolasco, A. | Palacios-Rojas, N.
Biochemical and chemical analysis of seeds and green plant tissues are essential in several breeding programs including nutritional and industrial enhancement, plant physiology, and plant pathology. The objective of CIMMYT’s maize nutritional quality and plant analysis laboratory is to develop and/or adopt methodologies to support the breeding programs. Therefore, wherever possible, we seek robust, cheap, and fast methodologies that allow us to provide accurate data to breeders for further field decisions. Ninety-six well microplate readers and near infrared reflectance (NIR) are two of the options we are currently using in the establishment of the biochemical and chemical platform for analysis. All methodologies presented here have been validated either by comparison with other methods or by inter-laboratory assessments. This manual compiles all current methodologies used in the laboratory and aims to serve as a guide for other laboratories or institutions that are interested in the methodologies. For quality protein maize assurance we present the tryptophan, lysine, and protein determination protocols. For provitamin A enhancement, the high performance liquid chromatography (HPLC) carotenoid protocol is included. For micronutrient analysis we present current iron and zinc determination by inductively coupled plasma-optical emission spectroscopy (ICP-OES). Other analysis performed in our lab are soluble sugars determination, anthocyanin content, total and free phenols, ash content, oil content, starch determination and amylase/amylopectin determination.
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This bibliographic record has been provided by International Maize and Wheat Improvement Centre