Detection and the Prevalence of Prune Dwarf Virus (PDV) in Important Cherry Plantations of the Eastern Mediterranean Region by DAS-ELISA and RT-PCR Analyzes
2022
Peli̇n Gündoğan | Mona Gazel | Kadri̇ye Çağlayan
In this study, the prevalence of PDV, which infects cherry (Prunus avium L.) trees and is among the important viral pathogens in Adana, Kahramanmaraş and Osmaniye provinces where cherry is extensively produced in the Eastern Mediterranean region of Turkey, was investigated. A total of 173 cherry leaf samples with and without symptoms collected from Adana, Kahramanmaraş and Osmaniye provinces were tested by DAS-ELISA and RT-PCR methods. PDV was not detected in all samples tested by the DAS-ELISA method. As a result of the tests performed with RT-PCR, 874 bp long nucleotide sequences belonging to the coat protein gene region of the 15 samples were obtained and found positive. After the sequences were obtained, they were compared with the sequences from Turkey and the sequences of PDV reference isolates registered in GenBank. As a result of BLASTn analysis, it was determined that the Turkish PDV cherry isolates showed similarity between 89.58-99.32%25 at the nucleotide level with the PDV reference sequence. Phylogenetic analysis of the nucleotide sequences of the obtained isolates was performed and their relationship with PDV cherry isolates in the world and Turkey was determined.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Pen Academic Publishing