Development and Validation of Analytical Methods for Quantification of L-Dopa%253B Application to Plant Materials
2023
Erten Akbel | İbrahim Bulduk
An essential neurotransmitter used to treat nervous system dysfunction like Parkinson%252339%253Bs disease is L-dopa. The amount of L-dopa in plant materials has been measured in this study using chromatographic and spectrophotometric methods that are user-friendly, straightforward, quick, and affordable. Utilizing conventional instrumental parameters, high-performance liquid chromatography and spectrophotometry methods were established. In accordance with ICH guidelines, these analytical methods were validated for linearity, accuracy, precision, and robustness. The chromatographic procedure made use of an Agilent Extend C18 (250times%253B4.6 mm, 5 micro%253Bm) column. 0.1%25 trifloroacetic acid solution and acetonitrile (92%252F8, v%252Fv) were used as mobile phase. It was run in isocrotic mode and the flow rate was 1 mL min-1. The retention time of L-dopa was determined as 3.85 minutes. L-dopa was identified using the spectrophotometric method, which involved measuring the solutions%252339%253B absorbance at a wavelength of 280 nm. In spectrophotometric analysis, ultra-pure water as a solvent gave sufficient molar absorptivity at a lambda%253Bmax of 280 nm. The results showed that spectrophotometric and chromatographic methods were linear, accurate, precise, robust, and the percent recovery was within standard limits. No statistically significant distinction existed between the methods within the 95%25 confidence interval (plt%253B0.05). The developed methods can be used to quantify L-dopa in plant materials for routine analysis and have been found to be very efficient.
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