Molecular identification and quality evaluation of commercial Ganoderma
2025
Leijiao Liu | Lihui Wei | Jing Xu | Hui Xiong | Shasha Zhou | Shuangcheng Ma | Wei Sun | Lixia Tian | Zhenhao Li | Zhichao Xu
Ganoderma has long been renowned in oriental countries as a prominent medicinal and edible supplement, with the products holding a substantial market demand. However, there remains a lack of systematic and comprehensive studies on the quality evaluation of commercial Ganoderma. In this study, we analyzed 84 batches of Ganoderma, including the fruiting bodies and spores, sourced from various markets. DNA barcoding using the ITS2 marker was employed and demonstrated excellent discriminatory capacity, allowing for the precise identification of 16 adulterants among the 84 Ganoderma. According to the standard detection methods for total polysaccharides and triterpenes outlined in the Chinese Pharmacopoeia, remarkable variations in the tested Ganoderma samples were observed; the total polysaccharide contents ranged from 0.22% to 3.31%, while the total triterpenes levels varied from 0.21% to 10.56%. Furthermore, LC-MS/MS quantification based on targeted ganoderic acids for Ganoderma revealed triterpene contents ranging from 0.01% to 0.98%, highlighting the necessity for regulating the quality of Ganoderma in the market. A comprehensive evaluation system for commercial Ganoderma is proposed, integrating molecular identification and mass spectrometry quantification.
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