Fine mapping and cloning of the sterility gene Bra2Ms in non-heading Chinese cabbage (Brassica rapa ssp. chinensis)
2024
Liping Song | Xia Li | Liguang Tang | Chuying Yu | Bincai Wang | Changbin Gao | Yanfeng Xie | Xueli Zhang | Junliang Wang | Chufa Lin | Aihua Wang
The application of amale-sterile line is an ideal approach for hybrid seed production innon-heading Chinese cabbage (Brassica rapa ssp. chinensis). However, the molecular mechanisms underlyingmale sterility in B. rapa are still largely unclear. We previously obtained the natural male sterileline WS24-3 of non-heading Chinese cabbage and located the male sterile locus, Bra2Ms,on the A2 chromosome. Cytologicalobservations revealed that the male sterility of WS24-3 resulted fromdisruption of the meiosis process during pollen formation. Fine mapping of Bra2Ms delimited thelocus within a physical distance of about 129 kb on the A2 chromosome of B. rapa. The Bra039753 geneencodes a plant homeodomain (PHD)-finger protein and is considered a potentialcandidate gene for Bra2Ms. Bra039753 was significantly downregulated in sterile line WS24-3 compared to the fertileline at the meiotic anther stage. Sequence analysis of Bra039753 identified a 369 bp fragmentinsertion in the first exon in male sterile plants, which led to an amino acidinsertion in the Bra039753 protein. Inaddition, the 369 bp fragment insertion was found to cosegregate with the malesterility trait. This study identified anovel locus related to male sterility in non-heading Chinese cabbage, and themolecular marker obtained in this study will be beneficial for themarker-assisted selection of excellent sterile lines in non-heading Chinesecabbage and other Brassica crops.
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