Carrot (Daucus carota L . ssp. sativus (Hoffm.) Arcang.) as source of antioxidants | Korenje (Daucus carota L. ssp. sativus (Hoffm.) Arcang.) kot vir antioksidantov

English. Carrot (Daucus carota L. s sp. sativus (Hoffm.) Arcang.) is a significant source of vitamins (A, B, C) and beta carotene. Further it contains vitamins B, C, E, H, folic acid and pantothenic acid. Carrot is an important source of trace elements (K, Na, Ca, Mg, P, S, Mn, Fe, Cu and Zn). Consumption of carrot improves eyesight, lowers cholesterol and improves digestion. In this work we evaluated and compared content of total polyphenols, ß-caroteneand antioxidant activity in five varieties of carrot (’Jitka’, Kardila’, Katlen , Rubína’ and Koloseum) Samples of carrot were collected at full maturity stages from area of Bardejov. Samples of fresh carrot were homogenized (25 g) in 50 ml 80 % ethanol and analysed after sixteen hours. The content of the total polyphenols was determined by using the Folin-Ciocalteu reagent (FCR). The content of ß-carotene was determined spectrophotometricaly at 450 nm. Antioxidant activity was measured using a compound DPPH (2.2-diphenyl-1-picrylhydrazyl) at 515.6 nm using spectrophotometer. Total polyphenols content in samples ranged from 81.25 ± 13.11 mg/kg to 113.69 ± 11.57 mg/kg and content of ß-carotenes ranged from 24.58 ± 2.38 mg/kg to 124.28 ± 3.54 mg/kg. We also evaluated and compared the antioxidant activity in selected varieties of ca rrot, which varied from 6.88 ± 0.92 % to 9.83 ± 0.62 %. Statistically si gnificant the highest value of total polyphenols was recorded in variety of Koloseum (113.69 ± 11.57 mg/kg). This variety is also character ized by the highest content of ß- carotene (124.28 ± 3.54 mg/kg) as well as the highest value of antioxidant activity (9.83 ± 0.62 %).

Slovenian. Korenje (Daucus carota L. ssp. sativus (Hoffm.) Arcang.) je pomemben vir vitaminov (A, B, C) in beta karotena. Dodatno vsebuje vitamine B, C, E, H, folno in pantotentsko kislino. Korenje je tudi pomemben vir elementov v sledeh kot so K, Na, Ca, Mg, P, S, Mn, Fe, Cu in Zn. Uživanje korenja izboljšuje vid, zmanjšuje količino holesterola in izboljšuje prebavo. V tej raziskavi smo ovrednotili in primerjali vsebnost celokupnih polifenolov, beta karotena in antioksidacijsko aktivnost v petih sortah korenja (’Jitka’, ‘Kardila’, ̓Katlen ̓, Rubína’ and ̓Koloseum’). Vzorci korenja so bili nabrani ob tehnološki zrelosti na območju Bardejova. Vzorci svežega korenja so bili homogenizirani (25 g) v 50 ml 80 % etanola in analizirani po 16 urah. Vsebnost celokupnih polifenolov je bila določena z uporabo Folin-Ciocalteu reagenta (FCR). Vsebnost beta karotena je bila določena spektrofotometrično pri 450 nm. Tudi antioksidacijaka aktivnost je bila izmerjana spektrometrično z uporabo DPPH˙ (2.2-difeenil-1-pikcrilhidrazil) pri 515.6 nm. Vsebnost celukopnih polifenolov v vzorcih je bila med 81.25 ± 13.11 mg/kg in 113.69 ± 11.57 mg/kg, vsebnost ß-karotena pa med 24.58 ± 2.38 mg/kg in 124.28 ± 3.54 mg/kg. Ovrednotili in primerjali smo tudi antioksidacijsko aktivnost v izbranih sortah korenja, ki je bila med 6.88 ± 0.92 % in 9.83 ± 0.62 %. Največjo, statistično značilno vsebnost polifenolov smo izmerili pri sorti Koloseum (113.69 ± 11.57 mg/kg). Ta sorta je bila značilna tudi po največji vsebnosti ß- karotena (124.28 ± 3.54 mg/kg) kot tudi po največji antioksidacijski aktivnosti (9.83 ± 0.62 %).