Comparative study of diagnostic methods used for monitoring of common grape vine (Vitis vinifera L.) crown gall (Agrobacterium vitis Ophel & Kerr) in Slovenia | Primerjava diagnostičnih metod spremljanja pojava raka (Agrobacterium vitis Ophel & Kerr) žlahtne vinske trte (Vitis vinifera L.) v Sloveniji

English. Agrobacterium vitis causes common grape vine (Vitis vinifera L.) crown gall disease that destroyed a lot of Slovenian vineyards more than a decade ago. Eighty isolates of Agrobacterium spp. collected during monitoring in 2006 were identified as A. vitis and A. tumefacies by pehA and multiplex PCR method. Tumor-inducing capacity of these strains was assessed on test plants and with PCR methods for detection of the Ti plasmid responsible for tumor induction. With VCF3/VCR3 primer pair six false negatives and no false positives were detected. The high genetic diversity of pathogenic Agrobacterium spp. strains affects the performance of molecular methods, thus biological test should be performed where results from molecular methods are doubtful.

Slovenian. Bakterija Agrobacterium vitis je povzročitelj raka žlahtne vinske trte (Vitis vinifera L). Ta bolezen je uničila mnogo slovenskih vinogradov pred več kot desetimi leti. V sklopu spremljanja pojava bolezni smo leta 2006 izolirali 80 izolatov, ki smo jih s PCR metodama pehA in multipleks določili kot vrsti A. vitis in A. tumefaciens. Sposobnost sevov, da izzovejo nastanek tumorjev, smo ocenjevali na gostiteljskih rastlinah. Z molekularnimi metodami pa smo določali prisotnost plazmida Ti, povzročitelja nastanka tumorjev. Z metodo PCR smo ugotovili šest lažno negativnih patogenih sevov in nobenega lažno pozitivnega. Velika genetska raznolikost patogenih sevov Agrobacterium spp. vpliva na zanesljivost določanja z molekularnimi metodami, zato se v primeru dvomljivih rezultatov priporoča dodatna izvedba bioloških testov na rastlinah.