Protein pattern analysis in tolerant and susceptible wheat cultivars under salinity stress conditions | Anliza vzorca beljakovin v odporni in občutljivi sorti pšenice v razmerah slanostnega stresa

English. To investigate proteome pattern of wheat cultivars, young leaves were collected from tillering stage of seedlings two weeks after development of the salinity stress. The extraction of proteins from leaf tissue was done and two dimensional electrophoresis using IPG strips and SDS-PAGE in the control and salinity treatments were performed. In total, 198 and 203 protein spots were identified in tolerant (‘Moghan3’) and susceptible (‘Pishtaz’) cultivars respectively. Also, among these, spots number 21 and 22 were detected with significant IF in ‘Moghan3’ and ‘Pishtaz’ respectively. Two-stage mass spectrometry (MS/MS) was used to identify protein spots. Common identified proteins, including proteins involved in removal of oxidants, Calvin cycle proteins, proteins involved in light reaction of photosynthesis and proton transfer, and heat shock protein were identified on basis of the functional groups and their frequency. In total, ‘Moghan3’ maintained the stability of the structure and performance of carbon metabolism under stress better than susceptible cultivar. In addition, defense against oxidative stress induced by salinity stress was performed by 2-cys peroxiredoxin BAS1 and Cu-Zn SOD proteins that tolerant cultivar defended against oxidative stress better than the susceptible cultivar. The greatest strength of ‘Moghan3’ and major weakness in ‘Pishtaz’ are relying on the unique proteins formed under salinity stress for the removal of oxidants and to maintain the activity of the photosynthetic light reactions, respectively.

Slovenian. Za analizo proteomskega vzorca v dveh sortah pšenice so bili vzorčeni mladi listi v fazi bilčenja dva tedna po izpostavitvi slanostnemu stresu. Izvleček beljakovin iz listnih tkiv je bil narejen z dvodimenzionalno elektroforezo z uporabo IPG trakov in SDS-PAGE, za rastline iz kontrole in tiste v slanostnem stresu. Celokupno je bilo evidentiranih 198 beljakovinskih točk za odporno sorto (‘Moghan3’) in 203 beljakovinskih točk za občutljivo sorto (‘Pishtaz’). Med temi sta bili ugotovljeni beljakovinski točki št. 21 in 22 z značilnimi vrednostmi IF za ‘Moghan3’ in ‘Pishtaz’. Za določitev beljakovin v točkah je bila uporabljena dvofazna masna spektrometrija (MS/MS). Določene beljakovine so obsegale encime, ki so vključeni pri odstranjevanju oksidantov, encime Kalvinovega cikla, beljakovine, ki so udeležene v svetlobnih reakcijah fotosinteze in v protonskem transportu ter beljakovine vročinskega udara. Beljakovine so bile določene na osnovi funkcionalnih skupin in njihove frekvence. V splošnem je v stresnih razmerah odporna sorta ‘Moghan3’ ohranjala stabilnost zgradbe in poteka presnove ogljika bolje kot občutljiva sorta. Dodatno sta se za obrambo proti oksidacijskem stresu v razmerah slanosti inducirala dva proteina, 2-cis peroksiredoksin BAS1 in Cu-Zn SOD protein, ki sta odporno vrsto ščitila bolje kot občutljivo. Odpornost sorte ‘Moghan3’ in občutljivost sorte ‘Pishtaz’ na slanostni stres temelji na edinstvenem vorcu beljakovin, ki se tvorijo v razmerah slanosti za odpravljanje oksidantov in vzdrževanje aktivnosti svetlobnih rekacij fotosinteze.