Some important aspects in Moringa oleifera Lam. micropropagation | Nekateri pomembni vidiki v mikropropagaciji moringe (Moringa oleifera Lam.)

English. Type and source of explant as well as the type of cytokinin were important factors for successful moringa micropropagation. Explants obtained from in vitro grown plant materials were better than others obtained from soil growing seedlings. In addition, nodal segments were better than shoot cuttings in terms of number of shoots/ explants, frequency of shoot formation and number of nodes/shoot. While callus formation on the base of nodal segment on BAP containing media were higher than those of KIN, especially under the influence of high concentration as an aspect of vitrification, BAP was better than KIN in moringa multiplication. Low nutrient medium (half strength MS) supplemented with 0.5 mg l-1 IAA was essential for successful root formation. The suitable conditions for moringa micropropagation on full strength MS or SH may exert low stress and low need to raise the expression of SOD and POX. On the other side, stress due to over increase of chemical components of double MS medium or low nutrient content of half strength MS, B5 or WPM expressed the highest number and staining intensity of SOD and POX bands, vice versa was detected in case of CAT.

Slovenian. Vir in vrsta izsečkov kot tudi vrsta citokininov sta pomembna dejavnika za uspešno mikropropagacijo moringe. Izsečki, pridobljeni iz in vitro vzgojenih rastlin so bili boljši kot tisti pridobljeni iz sejank vzgojenih v tleh. Dodatno so bili izsečki nodijev boljši kot iz ostalih delov poganjka glede na število nastalih poganjkov na izseček, pogostost tvorbe poganjkov in število nodijev na poganjek. Tvorba kalusa na bazi nodialnega segmenta je bila boljša v gojišču z BAP kot v gojišču s KIN, še posebej zaradi vpliva večjih koncentracij je bil pri mikropropagaciji moringe z vidika vitrifikacije BAP boljši kot KIN. Gojišče z majhno vsebnostjo hranil  (polovični MS) z dodatkom 0,5 mg l-1 IAA je bilo odločilno za tvorbo korenin. Primerne razmere za mikropropagacijo moringe na polnomočnih gojiščih MS ali SH so manj stresne in ne vzpodbudijo tvorbo SOD in POX. Po drugi strani je stres zaradi povečanja spojin v dvojnem gojišču MS ali v polovičnih gojiščih MS, B5 ali WPM z majhno količino hranil vzpodbudil največjo ekspresijo SOD in POX , kar se je pokazalo z največjim številom in močnejšo obarvanostjo eletroforeznih trakov, obratno je bilo ugotovljeno v primeru CAT.