Expression of IRT1 gene in barley seedlings under zinc deficiency at optimal and low temperatures | Izražanje IRT1 gena v sejankah ječmena ob pomanjkanju cinka pri optimalnih in nizkih temperaturah

English. The deficiency or excess of zinc (Zn) cause negative effect on plant metabolism and development. Therefore, plants have established a tightly controlled system, including protein transporters to balance the uptake and utilization of metal ions. In this study, the relative expression of HvIRT1 gene, encoding the transmembrane protein IRT1 was analyzed in shoots and roots of barley (Hordeum vulgare ‘Nur’) under zinc deficiency at optimal (22 °C) or low (4 °C) temperatures. The Zn deficiency (0 μmol) caused an increase in HvIRT1 gene expression under both optimal temperature condition and cold. Although, the difference in mRNA content of HvIRT1 gene in roots of barley under optimal and low temperature was not observe. However, the HvIRT1 expression in leaves was higher at optimal temperature compare with cold condition. Moreover, long-term (7 days) of low temperature influence along with zinc deficiency leads to a significant decrease in the amount of HvIRT1transcripts in leaves, that corresponds to a decrease of photosynthesis rate and biomass accumulation. Overall, these findings suggest that HvIRT1 gene play an important role in plant’s response to zinc deficiency under optimal temperatures condition as well as at cold.

Slovenian. Pomanjkanje ali prebitek cinka (Zn) povzročata negativne učinke na presnovo in razvoj rasttlin. Zaradi tega so rastline razvile dobro nadzorovan sistem, vključno s proteinskimi transporterji za uravnavanje privzema in porabe kovinskih ionov. V raziskavi je bilo analizirano izražanje HvIRT1 gena, ki kodira transmembranski protein IRT1 v poganjkih in koreninah ječmena (Hordeum vulgare ‘Nur’) ob pomankanju zinka pri optimalni (22 °C) in nizki (4 °C) temperaturi. Pomanjkanje cinka (0 μmol) je povzročilo povečano izražanje HvIRT1 gena pri optimalni kot pri nizki temperaturi. Razlika v vsebnosti mRNK HvIRT1 gena v koreninah ječmena v optimalnih razmerah in pri nizki temperaturi ni bila opažena, a kljub temu je bilo izražanje gena HvIRT1 v listih večje pri optimalni temperaturi v primerjavi s hladnimi rastnimi razmerami. Daljša izpostavitev (7 dni) nizki temperature je ob pomanjkanju cinka povzročila značilno zmanšanje transkriptov HvIRT1 v listih, kar ustreza upadu fotosinteze in akumulacije biomase. Ta odkritja nakazujejo, da igra HvIRT1 gen pomembno vlogo pri odzivu rastlin na pomanjkanje cinka tako v optimalnih razmerah kot pri nizkih temperaturah.