Flower bud sampling for meiotic slide preparations | Problematika nabiranja cvetnih brstov za izdelavo preparatov mejoze
2011
SINKOVIČ, Tomaž
English. Evolutionary aspect of meiosis and reasons for dominate sexual reproduction in higher plants are described. Conjugation of homologue chromosomes and recombination of genes in prophase of the first meiotic division are presented. Evolution of meiosis from mitosis is discussed. The problems of flower bud sampling for meiotic preparations are presented. Flower buds of Allium ursinum were collected in March and April 2008. Best results were obtained by sampling flower buds in the third week of March. For meiotic preparations the optimal length of flower buds was 1 to 1.5 cm. In April the flower buds were over-developed and we could observe developing pollen grains only.
Show more [+] Less [-]Slovenian. Opisan je evolucijski pomen mejoze in vzroki za prevlado spolnega načina razmnoževanja pri višjih rastlinah ter pomen prerazporejanja genov (rekombinacija) v profazi prve zoritvene delitve. Predstavljen je razvoj mejoze iz mitoze in razlike med tema celičnima delitvama. Opisano je nabiranje cvetnih brstov čemaža za pripravo mejotskih preparatov. Cvetne brste čemaža smo nabirali v marcu in aprilu 2008. Največ mejotskih faz smo opazili pri cvetnih brstih nabranih v tretjem tednu v mesecu marcu. Optimalna dolžina cvetnih brstov je bila 1 do 1,5 cm. V aprilu so bili cvetni brsti preveč razviti, lahko smo opazovali le pelodna zrna.
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