Butyrate production of anaerobic rumen bacterium Pseudobutyrivibrio xylanivorans Mz5T | Produkcija butirata pri anaerobni vampni bakteriji Pseudobutyrivibrio xylanivorans Mz5T

English. Butyrate is an important probiotic factor that beneficially affects the activity and survival of colonocytes and exerts an anticarcinogenic activity in the colon, too. Recently, on the basis of 17 rumen strains a new species P. xylanivorans with type strain Mz5T was established. The strain Mz5T produced butyrate and lactate as major fermentation products and utilized acetate in the medium with oat spelts xylan. Butyrate production was strongly increased during the logarithmic growth phase and was still slowly increasing in the late stationary phase. The rate of butyrate production was similar to other carbohydrates (birchwood and beechwood xylan, xylose, arabinose and glucose). The bulk of butyrate with regard to lactate was formed during the early logaritmic phase regardless of substrate. Therefore, P. xylanivorans Mz5T could be used as probiotic in animal feed to improve xylan degradation and also for increased production of butyrate in the colon. The best choice would be a culture in the early logarithmic phase grown on an inexpensive source of carbohydrates.

Slovenian. Butirat je pomemben probiotski dejavnik, ki ugodno vpliva na delovanje in preživetje kolonocitov ter v debelem črevesu deluje tudi antikancerogeno. Pred kratkim je bila na osnovi 17 sevov, izoliranih iz vampa, opisana nova vrsta P. xylanivorans s tipskim sevom Mz5T. Sev Mz5T je na ksilanu ovsenih plev produciral butirat in laktat kot glavna produkta fermentacije in pri tem porabljal acetat. Produkcija butirata je močno narasla med logaritemsko fazo in se je še v pozni stacionarni fazi počasi povečevala. Na drugih ogljikovih hidratih (na brezovem in bukovem ksilanu, ksilozi, arabinozi in glukozi) je bila kinetika tvorbe butirata podobna. Največ butirata glede na laktat je nastajalo med zgodnjo logaritemsko fazo neodvisno od substrata. P. xylanivorans Mz5T bi torej lahko uporabili kot probiotik v prehrani živali tako za izboljšanje razgradnje ksilana kot tudi za povečano produkcijo butirata v debelem črevesu. Najbolje bi bilo uporabiti kulturo v zgodnji logaritemski fazi rasti, ki bi jo lahko namnožili na cenenem ogljikohidratnem viru.