Isolation and characterization of bacteriophages of rumen bacterium Prevotella bryantii TC1-1 | Osamitev in opis bakteriofagov vampne bakterije Prevotella bryantii TC1-1

English. Sampling, inoculation and extraction procedures were elaborated for the isolation of bacteriophages from cattle rumen fluid. Several strains from four different species of rumen bacterial genus Prevotella were used as indicator organisms as well as Escherichia coli, Salmonella sp. and Yersinia enterocolitica strains. Only one strain, i.e. P.bryantii TC1-1 was successfully infected with filter sterilised rumen fluid inoculum containing rumen bacteriophages. Two plaque morphotypes were observed, both being turbid and rather small but clearly different in size. The preliminary transmission electron microscopy analysis showed that all observed bacteriophages have presumably icosahedral symmetry. The infection trials of other Prevotella strains with isolated bacteriophages from the strain TC1-1 were successful only with type strains of species P.bryanti and P.brevis respectively. Bacteriophage purification and long term storage procedures were elaborated.

Slovenian. Pripravili in opisali smo postopke vzorčenja, okužbe in ekstrakcije za osamitev bakteriofagov iz kravjega vampnega soka. Za indikatorske organizme smo uporabili več sevov iz štirih vrst vampnega bakterijskega rodu Prevotella ter seve vrst Escherichia coli, Salmonella sp. in Yersinia enterocolitica. Z vzorcem filtrsko steriliziranega vampnega soka, ki je vseboval vampne bakteriofage, smo uspešno okužili le en indikatorski sev, to je P.bryantii TC1-1. Opazili smo dve morfološki obliki plakov, obe motni in dokaj majhni. Začetni pregledi bakteriofagov s presevno elektronsko mikroskopijo so pokazali, da so vsi pregledani bakteriofagi najverjetneje ikozaedrično simetrični. Z bakteriofagi, izoliranimi iz seva P.bryantii TC1-1, smo uspešno okužili tudi tipski sev iste vrste in tipski sev vrste P.brevis. Preskusi okužbe drugih sevov iz rodu Prevotella ter ostalih indikatorskih organizmov so bili neuspešni. Pripravili smo postopke čiščenja in shranjevanja vampnih bakteriofagov.