Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
2020
Martin Vollár | Gábor Feigl | Dóra Oláh | Attila Horváth | Árpád Molnár | Norbert Kúsz | Attila Ördög | Dezső Csupor | Zsuzsanna Kolbert
Similar to animals, it has recently been proven that nitro-fatty acids such as nitro-linolenic acid and nitro-oleic acid (NO2-OA) have relevant physiological roles as signalling molecules also in plants. Although NO2-OA is of great therapeutic importance, its presence in plants as a free fatty acid has not been observed so far. Since Brassica napus (oilseed rape) is a crop with high oleic acid content, the abundance of NO2-OA in its tissues can be assumed. Therefore, we quantified NO2-OA in B. napus seeds and differently developed seedlings. In all samples, NO2-OA was detectable at nanomolar concentrations. The seeds showed the highest NO2-OA content, which decreased during germination. In contrast, nitric oxide (&bull:NO) levels increased in the early stages of germination and seedling growth. Exogenous NO2-OA treatment (100 µ:M, 24 h) of Brassica seeds resulted in significantly increased &bull:NO level and induced germination capacity compared to untreated seeds. The results of in vitro approaches (4-Amino-5-methylamino-2&prime:,7&prime:-difluorofluorescein (DAF-FM) fluorescence, &bull:NO-sensitive electrode) supported the &bull:NO liberating capacity of NO2-OA. We observed for the first time that Brassica seeds and seedlings contain free NO2-OA which may be involved in germination as an &bull:NO donor as suggested both by the results of exogenous NO2-OA treatment of seeds and in vitro approaches. Due to their high NO2-OA content, Brassica sprouts can be considered as a good source of dietary NO2-OA intake.
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