Validation of a Method for Anacardic Acid Quantification in Cashew Peduncles via High-Performance Liquid Chromatography Coupled to a Diode-Array Detector
2023
Francisco Oiram Filho | Morgana Pereira Mitri | Guilherme Julião Zocolo | Kirley Marques Canuto | Edy Sousa de Brito
The cashew peduncle has a high nutritional value and contains a wide variety of phenolic compounds. Among these, anacardic acids (AnAc) are biologically active components: however, they influence the cashew juice flavor and, consequently, its acceptance. This study validates a high-performance liquid chromatography method for quantifying the AnAc present in cashew peduncles, using a C18 reverse-phase column and a diode-array detector. The calibration curve obtained showed satisfactory precision for intraday (CV = 0.20%) and interday (CV = 0.29%) quantification, linearity (y = 2333.5x + 2956.2: r2 = 0.9979), repeatability with respect to retention time (CV = 0.45%) and area (CV = 0.30%), and selectivity, and possessed detection and quantification limits of 0.18 and 0.85 µ:g·:mL&minus:1, respectively. Different cashew clones containing AnAc were extracted and analyzed using the proposed method. A recovery of >:90% was achieved using two sequential extractions. The total AnAc content ranged from 128.35 to 217.00 mg·:100 g&minus:1 in peduncle samples obtained from five different cashew clones.
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