Effects of Genetic Polymorphism in the IFI27 Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells
2024
Xinyi Jiang | Zhihui Zhao | Xuanxu Chen | Fengshuai Miao | Jing Li | Haibin Yu | Ping Jiang | Ziwei Lin
Milk fat is an important indicator for evaluating milk quality and a symbol of the core competitiveness of the dairy industry. It can be improved through genetic and feed management factors. Interferon alpha-inducible protein 27 (IFI27) was found to be differentially expressed when comparing the transcriptome in high- and low-fat bovine mammary epithelial cells (bMECs) in our previous research. Therefore, this study aimed to investigate whether the IFI27 gene had a regulatory effect on lipid metabolism.We detected six SNPs in the IFI27 gene (UTR-(-127) C>:A, UTR-(-105) T>:A, UTR-(-87) G>:A, I1-763 G>:T, E2-77 G>:A, E2-127 G>:T) in a Chinese Holstein cow population. Association analysis of the polymorphism of IFI27 and milk quality traits showed that the AG and GG genotype of E2-77 G>:A, and the GG and TT genotypes of E2-127 G>:T were connected to milk fat (p <: 0.05). Haplotype frequency analysis showed that H5H5 was associated with lower milk fat content (p <: 0.05), while milk from H5H6 animals had a higher fat content (p <: 0.05). Subsequently, IFI27 overexpression vectors (PBI-CMV3-IFI27) and interference vectors (Pb7sk-GFP-shRNA) were constructed. Overexpression of the IFI27 gene in bMECs caused a significant increase in triglycerides (TGs) content (p <: 0.05) and decreases in cholesterol (CHOL) and nonestesterified fatty acid (NEFA) content (p <: 0.05), while interference with IFI27 expression produced opposing changes (p <: 0.05). In summary, IFI27 E2-77 G>:A and IFI27 E2-127 G>:T may be useful as molecular markers in dairy cattle to measure milk fat, and the IFI27 gene may play an important role in milk lipid metabolism.
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