Integrating Genome and Transcriptome-Wide Data to Explore the Expression Dynamics of ABCDE-like MADS-Box Genes in Phoebe bournei Floral Organs
2025
Yuanyang Bi | Houhua Fu | Zihan Jiang | Yan Jiang | Limei You | Can Li | Xiongde Tu | Sagheer Ahmad | Zhongjian Liu | Shipin Chen | Wenjun Lin
The MADS-box transcription factor gene family is essential for the differentiation and development of floral organs. Genome-wide and transcriptome data from Phoebe bournei provide the foundation for studying its floral development. In this study, phylogenetic relationships, protein characteristics, conserved domains, gene structures, and cis-acting elements in promoter regions of P. bournei MADS-box genes were systematically analyzed through bioinformatics methodologies. A total of 69 PbMADS genes were identified and classified into 15 subfamilies based on their phylogenetic relationships. The potential functions of these genes were inferred based on transcriptomic data and GO classification. In addition, we selected genes from the ABCDE model to analyze protein interactions and performed RT-qPCR to analyze their expression in each floral organ whorl (outer tepals, inner tepals, stamens, and carpel). Based on these analyses, we rationally constructed a model for the floral organ development in P. bournei. This study, thus, provides a theoretical reference for germplasm innovation in P. bournei and offers insights into the floral development in other Lauraceae species.
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