CO2 Enrichment Alters the Phytochemical Composition of Centella asiatica: GC-MS Analysis

Centella asiatica (L.) Urban is a medicinal herb containing valuable bioactive compounds widely used in pharmaceutical, cosmetic, and traditional medicine applications. This study investigated the effects of elevated CO2 levels (1000, 800, and 600 ppm compared to ambient ~420 ppm) on secondary metabolite composition in C. asiatica using GC-MS analysis of ethyl acetate extracts. Significant treatment effects (p &lt: 0.001) were observed across nine identified compounds, with &alpha:-copaene showing the most pronounced response. At 1000 ppm CO2, sesquiterpene hydrocarbons, including &alpha:-copaene (10.60%) and trans-caryophyllene (8.97%), reached their highest concentrations, representing 232% and 413% increases over ambient conditions, respectively. Germacrene D demonstrated optimal synthesis at 800 ppm (8.12%) while remaining undetectable under ambient conditions. In contrast, the diterpene neophytadiene (16.84%) and the oxygenated sesquiterpene caryophyllene oxide (11.27%) exhibited maximum concentrations under ambient conditions. Principal Component Analysis confirmed distinct metabolic profiles, with the first two components explaining 84.38% of the total variance. Correlation analysis revealed strong positive relationships (r &gt: 0.85, p &lt: 0.001) between structurally related sesquiterpenes. These findings establish a foundation for optimizing cultivation conditions to enhance specific bioactive compound production in C. asiatica, with potential applications in pharmaceutical production systems targeting sesquiterpene-derived medicines. The research demonstrates that atmospheric CO2 modulation offers a promising strategy for targeted enhancement of secondary metabolite synthesis, though further investigation of molecular mechanisms and environmental interactions is necessary for commercial implementation.