Integration of Green and Far-Red Light with Red-Blue Light Enhances Shoot Multiplication in Micropropagated Strawberry
2025
Yali Li | Ping Huang | Xia Qiu | Feiyu Zhu | Hongwen Chen | Si Wang | Jiaxian He | Yadan Pang | Hui Ma | Fang Wang
Light spectral composition critically regulates plant morphogenesis and molecular adaptation in controlled environments. This study investigated the synergistic effects of three light spectra, red-blue (RB, 7:3), red-blue-green (RGB, 7:3:1), and red-blue-far-red (RBFR, 7:3:1), on multiplication, morphogenesis, physiological traits, and transcriptomic dynamics in tissue-cultured strawberry (Fragaria ×: ananassa cv. &lsquo:Benihoppe&rsquo:). After 28 days of cultivation under controlled conditions (25 °:C/22 °:C day/night, 50 &mu:mol·:m&minus:2·:s&minus:1 PPFD), RBFR and RGB treatments significantly enhanced shoot multiplication (38.8% and 24.2%, respectively), plant height, and callus biomass compared to RB light. RGB elevated chlorophyll a and b by 1.8- and 1.6-fold, respectively, while RBFR increased soluble protein content by 16%. Transcriptome analysis identified 144 and 376 differentially expressed genes (DEGs) under RGB and RBFR, respectively, enriched in pathways linked to circadian rhythm, auxin transport, and photosynthesis. Far-red light upregulated light signaling and photomorphogenesis genes, whereas green light enhanced chlorophyll biosynthesis while suppressing stress-responsive genes. These findings elucidate the spectral-specific regulatory mechanisms underlying strawberry micropropagation and provide a framework for optimizing multispectral LED systems in controlled-environment horticulture.
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