A one-step enzyme-linked immunosorbent assay for fipronil detection
2026
Yujing Li | Erjing Zhang | Hejie Li | Yan Zhang | Jing Zhang | Chunsheng Li | Jingjing Liu
A rapid enzyme-linked immunosorbent assay (ELISA) system for the detection of fipronil was studied. Artificial antigens were synthesized through the glutaraldehyde method, and monoclonal antibodies were obtained via immunization and fusion techniques. The concentration of the coated antigen and antibody and the reaction time were determined, and the recovery rate was determined by an ELISA detection system. The cross-reaction rate of the antibody against fipronil metabolites was lower than 21.46%. The response time of the selected ELISA system was 30 min shorter than that of the competitive ELISA system. The IC20 to IC80 of the standard curve ranged from 0.139 to 1.433 μg/L. The recovery rate of fipronil spiked in eggs was 76.7%–93.7%. This study provides support for the rapid ELISA of fipronil.
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