miR825-5p-regulated TNLs govern Arabidopsis resistance to Tetranychus urticae and Pieris brassicae
2025
Rosa-Diaz, Irene | Lopez-Marquez, Diego | Beuzon, Carmen R | Diaz, Isabel | Ministerio de Ciencia e Innovación (España) | European Union Center (US)
MicroRNAs are essential regulators in plant resistance to biotic stresses, but their specific roles in the plant-herbivore context require deeper investigation. Here, we studied how the Arabidopsis miR825-5p differentially modulates certain TNLs (MRT1, MRT2, and MIST1), triggering defensive responses against the sucking acari Tetranychus urticae or the chewing insect Pieris brassicae. We demonstrated that the expression of miR825-5p is downregulated following T. urticae and P. brassicae infestation and identified miRNA TNL targets whose expression is induced by both feeders. miR825-5p downregulates MRT1 and MRT2, acting as a negative modulator of Arabidopsis basal resistance against T. urticae. A similar miR825-5p-mediated regulation of basal resistance, including MRT1 and MRT2 but also MIST1 silencing, is involved in the Arabidopsis response against P. brassicae. Moreover, miR825-5p triggered the production of MIST1-derived secondary interfering RNAs (phasiRNAs) and amplified silencing of MRT1 and MRT2. Taken together, our findings reveal the role of the miR825-5p/TNL module in controlling Arabidopsis response and adapting plant defenses based on the specific threat.
Show more [+] Less [-]This work was supported by grants PID2020-115219RB-I00 and PDC32021-121055-100, funded by MCIN/AEI/10.13039/501100011033 as appropriate, by ‘ERDFER A way of making Europe’ and by the ‘European Union’. PRE2018-083375 from MCIN/AEI supported IRD
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