Trout gill cells in primary culture on solid and permeable supports
2007
Leguen, Isabelle | Cauty, Chantal | Odjo, Nestor | Corlu, A. | Prunet, Patrick | Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) ; Institut National de la Recherche Agronomique (INRA) | Régulations des équilibres fonctionnels du foie normal et pathologique ; Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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Show more [+] Less [-]English. Trout gill cells in primary culture on solid and permeable supports were compared. Cultures were carried out by directly seeding cells on each support after gill dissociation. Most of the cell types present in culture were similar, regardless of culture support (pavement cells, mucous cells (3-4%), but no mitochondria-rich cells). However, insertion of mucous cells in cultured epithelium on permeable support presented a morphology more similar to gills in situ. Gene expression of ion transporters and hormonal receptors indicated similar mRNA levels in both systems. Cortisol inhibited cell proliferation on both supports and maintained or increased the total cell number on solid and permeable membranes, respectively. This inhibition of mitosis associated with an increase or maintenance of total gill cells suggests that cortisol reduced cell degeneration. In the presence of cortisol, transepithelial resistance of cultured gill cells on permeable membranes was increased and maintained for a longer time in culture. In conclusion, gill cells in primary culture on permeable support present: (i) a morphology more similar to epithelium in situ; and (ii) specific responses to cortisol treatment. New findings and differences with previous studies on primary cultures of trout gill cells on permeable membrane are discussed.
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