Tag-sample lookup files for plant and animal metabarcoding data from 14 Holocene lake sediment records from the European Alps [Dataset]

One file is for demultiplexing the plant metabarcoding dataset, generated using the trnL p6-loop gh primers of Taberlet et al. The other is for the animal metabarcoding dataset, generated using the 16S MamP007 primers of Giguet-Covex et al. Each file contains forward and reverse primer tags for eight PCR replicates applied to 844 sediment samples or controls, together with European Nucleotide Archive (ENA) accession codes for their associated amplicon libraries, which are publicly available at ENA projects PRJEB85748 and PRJEB52290. Sequencing facilities GSCT: Genomics Support Centre Tromsø TMU: The Arctic University Museum of Norway NCFS: Norwegian College of Fishery Science

I.G.A. and Y.L. were supported by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 819192) and the ECOGEN project “Ecosystem change and species persistence over time: A genome-based approach,” financed by Research Council of Norway grant 250963/F20, the latter also supported P.D.H., S.G.-P., and S.Z., most fieldwork, and other running costs. Open access funding was provided by UiT The Arctic University of Norway. S.G.-P. was also supported by the Beatriu de Pinós Programme (BP-2021-00131) and a fellowship from “la Caixa” Foundation (ID 100010434, fellowship code LCF/BQ/PI24/12040011). P.D.H. acknowledges support from the Knut and Alice Wallenberg Foundation (KAW 2021.0048 and KAW 2022.0033). Coring and stratigraphic analyses at Lake Sulzkar were funded through an Earth System Sciences grant from the Austrian Academy of Sciences: “Pulling the plug—Restoration of an Alpine lake”. Coring at Lakes Sangiatto, Hopschu, Emines, Bretaye, and Sulsseewli was supported by the Oeschger Centre for Climate Change Research (OCCR) as part of the Graduate School of Climate Sciences at the University of Bern. The PhyloAlps reference database was built thanks to the following projects: the joint ANR-SNF project Origin-Alps (ANR-16-CE93-0004, SNF-310030L_170059), European Research Council under the European Community’s Seventh Framework Programme FP7/2007-2013 grant agreement 281422 (TEEMBIO), and by the SNF grant 31003A_149508. The sequencing for the PhyloAlps reference database was performed within the framework of the PhyloAlps project, funded by France Génomique (ANR-10-INBS-09-08)

With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)

Peer reviewed