Screening a new European hake (Merluccius merluccius) chromosome-level genome assembly suggests an XX/XY sex determining system driven by the SRY-box transcription factor 3 (sox3)

13 pages, 4 figures, 4 tables

Sex determination is exceptionally diverse and shows a high evolutionary rate in fish. European hake (Merluccius merluccius) is a species of great commercial value distributed throughout European coasts, which displays a significant sexual growth dimorphism. We present a chromosome-level genome of M. merluccius, composed of 215 contigs using long- and short-read sequencing, further scaffolded into the species’ 21 chromosomes using Hi-C technique (715 Mb). RNA-seq on several tissues from pooled individuals improved annotation (26,625 protein-coding genes and 11,083 ncRNAs). Five males and 5 females from an Atlantic population were re-sequenced at 30× depth to look for association with sex across the whole genome. Genetic differentiation between males and females (FST) and intrapopulation fixation index (FIS) pointed to a region on chromosome 9 spanning ∼10 Mb which included several genes related to gonad differentiation and showed strong linkage disequilibrium associated with a putative inversion. Near sox3 gene (∼25 kb), SNPs were mostly heterozygous in males and homozygous in females, consistent with an XX/XY sex-determining (SD) system. These SNP markers were validated in a larger sample of 56 males and 63 females from the same population using MassARRAY. Other genomic regions that were differentiated between males and females and suggestive of sexual conflict were also explored across the genome. Results support a candidate master SD gene in M. merluccius and indicate some differentiated regions potentially under sexual conflict. This information will be useful for the fisheries management of M. merluccius in the context of climate change, where noninvasive sex identification tools are essential

This study was supported by the Regional Government Xunta de Galicia (Consellería de Cultura, Educación y Universidad, Vicepresidencia Segunda, Consellería de Economía, Empresa e Innovación; Spain) (grant number ED431C 2022/33). This research has been carried out under the framework of Spain's Recovery and Resilience Plan, and more specifically under the investment line number 1 of its component number 17, where the complementary RTDI plan with the autonomous regions of Spain is foreseen, with one of those being the Complementary RTDI Plan for Marine Science, which includes the Marine Science Program for Galicia. The DESCARSEL0922 scientific campaign by EU-Spain has been co-funded by the European Union through the European Maritime and Fisheries Fund within the National Program for the collection, management, and use of data in the fisheries sector, as well as support for scientific advice related to the Common Fisheries Policy and the DESCARSEL project. NP-M was supported by MSCA-PFFishAdapt 101066785 and MC by a contract within the framework of the State Plan for Scientific, Technical and Innovation Research 2021–2023 (PID2022-137821OB-C31)

Peer reviewed