Análise ultra-estrutural da túnica muscular do intestino delgado de cães preservado em diferentes meios | Ultrastructural evaluation of canine layer intestinal muscle mantained in different conservative solutions
2002
Mota, Francisco Cláudio Dantas | Eurides, Duvaldo | Beletti, Marcelo Emílio | Freitas, Patrícia Maria Coletto | Mastrantonio, Eneida César | Shimizu, Bianca Jacob | Cardoso, Julio Roquete | Martins, Alan Kardek
Portuguese. Foram avaliados 25 amostras da camada muscular do intestino delgado de cães, conservados em solução supersaturada de açúcar a 300%, glicerina a 98%, polivinil-pirrolidona a 5% e tintura de tiomersal 1:1000. As amostras foram mantidas conservadas por 45 dias e submetidas a análise ultra-estrutural por microscopia eletrônica de transmissão. Os meios não preservaram totalmente a integridade celular, sendo as soluções de açúcar a 300% e glicerina 98% as que melhor mantiveram as ultra-estruturas celulares.
Show more [+] Less [-]English. The present study evaluated twenty-five sarnples of the muscular layer of the small gut in dogs, which were preserved in a 300% sugar supersaturated. solution, 98% glycerin, 5% polyvinil-pirrolidona and tiomersal tincture at 1: 1000. The samples were maintained under conservation for 45 days and further submitted to ultrastructural assay by transmission electron microscopy. The cellular integrity was not entirely preserved when using the media, while the 300% sugar solution and 98% glycerin were the preservation conditions that better maintained the cellular ultrastructure.
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