Determination of mycotoxins in barley grain by FT-NIR Spectroscopy.
2014
Marta BRADÁČOVÁ | Kristýna BEZDĚKOVÁ | Pavlína SMUTNÁ
Our objective was to study the application of the Near Infrared Spectroscopy for determination of mycotoxins in spring barley grains. The samples of 10 spring barley cultivars from field trials with natural and artificial infection of Fusarium spp. were assayed. The content of mycotoxins in grains was analysed at the Institute of Chemical Technology, Prague by a reference method of ultra-high performance liquid chromatography coupled with ultra-high resolution mass spectrometry and obtained data were used for development of calibration models for two mycotoxins - deoxynivalenol and nivalenol. The models with correlation coefficient 0.85 for nivalenol and 0.82 for deoxynivalenol are applicable to preliminary screening of samples in the range of values assayed by the analytical method.Our objective was to study the application of the Near Infrared Spectroscopy for determination of mycotoxins in spring barley grains. The samples of 10 spring barley cultivars from field trials with natural and artificial infection of Fusarium spp. were assayed. The content of mycotoxins in grains was analysed at the Institute of Chemical Technology, Prague by a reference method of ultra-high performance liquid chromatography coupled with ultra-high resolution mass spectrometry and obtained data were used for development of calibration models for two mycotoxins - deoxynivalenol and nivalenol. The models with correlation coefficient 0.85 for nivalenol and 0.82 for deoxynivalenol are applicable to preliminary screening of samples in the range of values assayed by the analytical method.
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