<i>Coriolopsis trogii</i> MUT3379: A Novel Cell Factory for High-Yield Laccase Production
2024
Luca Mellere | Martina Bellasio | Francesca Berini | Flavia Marinelli | Jean Armengaud | Fabrizio Beltrametti
<i>Coriolopsis trogii</i> is a basidiomycete fungus which utilizes a large array of lignin-modifying enzymes to colonize and decompose dead wood. Its extracellular enzymatic arsenal includes laccases, i.e., polyphenol oxidases of relevant interest for different industrial applications thanks to their ability to oxidize a diverse range of natural and synthetic compounds. In this work, the production of laccases in <i>C. trogii</i> MUT3379 was explored and improved. From an initial production of ca. 10,000 U L<sup>−1</sup>, the fermentation process was gradually optimized, reaching a final yield of ca. 200,000 U L<sup>−1</sup>. An SDS-PAGE analysis of the secretome highlighted the presence of a main protein of ca. 60 kDa showing laccase activity, which was designated as Lac3379-1 once its primary sequence was established by tandem mass spectrometry. The characterization of Lac3379-1 revealed a remarkable enzymatic stability in the presence of surfactants and solvents and a diversified activity on a broad range of substrates, positioning it as an interesting tool for diverse biotechnological applications. The high-yield and robust production process indicates <i>C. trogii</i> MUT3379 as a promising cell factory for laccases, offering new perspectives for industrial applications of lignin-modifying enzymes.
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