Physalis (Physalis L.) is grown worldwide.  P. pubescens L. is most widespread in Russia. The analysis of development of biochemical properties in fruits of P. pubescens L. in temperate climate was presented in the article. The berries of physalis have acid reaction and high nutrient value due to polyphenols, carotenoids that give orange color, also contain moderate  quantity  of  vitamin  C.  Moreover  in  the  berry juice there are significant quantities of potassium, phosphorus, calcium, magnesium, zinc, copper, iron, manganese and boron. P. pubescens L. is a promising exotic  crop  that  can be used for  production  of functional  foodstuffs.   Physalis  berries  are  usually used as fresh vegetables for salad recipes, providing the sweet and sour balanced taste. The whole fruit berries are also processed  into  syrups or  dried,  so that they are regarded as ‘elegant raisins’. Our study showed that the dry matter content was 15.5% in cultivar ‘Zolotaya Rossiyp’ in temperate climate,  while the ascorbic acid content  was 9.9 mg per 100 g. of wet weight. In temperate climate 318 mg GAE/100 g. polyphenols were accumulated in the berry of P. pubescens L. The titrated acidity of ‘Zolotaya Rossiyp’  berries was 0.90%,  at  pH  4.72.  The level higher than 4 confirms the low  acidity. This can be explained by the fact of presence of organic acids as a free forms in the berry juice.