Fruit peel/pulp distribution of biologically active compounds is an important characteristic of plant physiology and the basis of zero waste production in agriculture. Among C. ficifolia, C. maxima and C. moschata the former showed the lowest dry matter content, especially in peel, similar peel and pulp values of antioxidant activity (AOA) and polyphenol content (TP), with the highest levels in fruit placenta. Peel carbohydrate profile of C. ficifolia fruit was characterized by lower levels of disaccharides compared to C. maxima and C. moschata peel and an opposite pattern of monosaccharides accumulation. The analysis of 25 elements content in Cucurbita peel and pulp, using ICP-MS, indicated that C. ficifolia fruit are characterized by significantly high concentrations of Sr, Si and I in pulp compared to the values of C. maxima and C. moschata. On the contrary, C. maxima and C. moschata were characterized by low concentration of pulp Mn. Highly significant positive correlations were recorded between Cr-Sr, Cr-Ca and CaSr (r=0.906; 0.939 and 0.974 respectively) and P-Cu (r=0.968). Despite C. ficifolia, does not contain carotenoids, it is highly valuable due to the high levels of Si, I, Cr and Ca in peel and pulp, which reveals new areas of its application.