Three varieties of onions have been created and included in the State Register of the Russian Federation for the first time in the Far East at Primorskaya Vegetable experimental station – Branch of the Federal State Budgetary Scientific Institution “Federal Scientific Vegetable Center” (PVES - Branch of the FSBSI FSVC). Dmitrich: Medium. The bulb is cross-elliptical, weighing 55-70 g. Dry scales are brown, their number is 2-3, juicy scales are white with a greenish tinge. The neck of a medium-sized, two- or threepart. It have semi-acute taste. Trading yields 162-177 kg/ha. Maximum yield of 270 kg/ha (Khabarovsk region). Ivashka: Medium. The bulb is round, weighing 50-60 g, dry scales are pink, their number is 2-3, juicy scales with a pinkish tinge. It have semi-acute taste. Rocket: Medium. The bulb is wide elliptical, weighing 50-60 g. Dry brown scales, their number 2-3, juicy scales are white. It have semi-acute taste. The method of hybridization created a promising variety Antey, which is transferred to the state variety testing: early (vegetation period when sowing seeds in the soil 105-115 days). The bulb is round (index 0.9-1.1), the color of juicy scales is white. Aging before cleaning – up to 95%, keeping the storage 180 days – up to 92%. It have semi-acute taste, the dry matter content of 9.5-11.0%. Relatively resistant to bacterial rots of the bulb. For today the basic line of grades, optimally suitable for agroclimatic conditions of region which will be used for further selection with use of modern molecular-genetic methods together with scientists of Russian State Agrarian University – Moscow Timiryazev Agricultural Academy and Japanese Partners in the RNF project.

Relevance. The creation of varieties with a complex of economically valuable traits, resistant to biotic and abiotic environmental factors, is the main direction in melon breeding. The market for vegetables and melons and gourds is very dynamic, the demands for varieties are constantly changing, therefore, it is necessary to introduce new varieties with high taste and commercial qualities, as well as capable of producing high yields in a changing climate. The aim of the work is to produce a new melon starting material for use in the breeding process.Materials and methods. At the Bikovskaya cucurbits breeding experimental station – branch of the Federal state budgetary scientific institution "Federal scientific vegetable center", research was carried out from 2018 to 2020. The object of research is samples from the collection of VIR, foreign and domestic selection, hybrid combinations obtained as a result of intervarietal hybridization. Collection samples of melon were studied in the nursery of the source material: 84 samples from the VIR collection, 26 samples of domestic and foreign selection were studied.Results. As a result of the research, samples of melon with economically valuable traits were identified that meet the requirements of selection. Paired crosses with zoned varieties of local selection were carried out with them. As a result of hybridization, hybrid combinations F1 were obtained, from which the best ones were selected for further breeding work. The obtained collection samples and hybrid combinations were evaluated in terms of taste, yield, resistance to environmental abiofactors, dry matter content, large-fruited, attractive color of the fruit and pulp As a result received a new source material for the selection of new varieties and hybrids of melons with valuable economic traits. The resulting hybrid combinations of F1 melon were tested against an infectious background. Hybrid combinations with the best performance will be further studied in a breeding nursery.

Relevance. Lettuce is a widespread green crop. The high demand for lettuce products, as well as for new varieties, drives the development of different breeding practices for this crop. It is the most promising to use hybridization method in the lettuce breeding for a diverse source material production. However, lettuce is a self-pollinating plant with small flowers and a short flowering period (about three hours), so the technique of castration and pollination is difficult. The current methods of lettuce hybridization vary in efficiency and labor intensity. This study aims to compare the effectiveness of three different hybridization methods and to obtain a source material for lettuce breeding using these methods.Materials and methods. 15 varieties of different variety types of lettuce were studied in various combinations. Three hybridization methods was conducted in the conditions of the Moscow region and the Krasnodar Territory: 1) using flies as pollinating insects, 2) using manual castration by cutting off the inflorescence of unopened buds with pollen inside and washing off pollen residues followed by manual pollination ("Clip and Wash" method), and 3) using manual emas- culation with a single pollen wash, also followed by manual pollination. The hybridity level assessment and the breeding material selection were performed according to the main morpho- logical and economically valuable characteristics.Results. In the period from 2021 to 2023, 529 hybrid plants were produced using three different hybridization methods of lettuce. Out of these, 150 plants of various variety types and forms were selected for further breeding. The "Clip and Wash" method was found to be the most effective in terms of achieving the highest hybridization percentage (92-100%) with less labor costs in organizing crosses. However, the hybridization process using insect pollination was less effective and required more labor, but it was also possible to pollinate a larger number of inflorescences using this method.

The main direction in the selection of sweet peppers is the creation of hybrids with a high heterosis effect. The question of components choice for the greatest effect in crossings is studied by breeders and geneticists  of many countries for a long time. However, until now it has not been possible to create a theory that allows breeders conducting practical work to consciously and  reliably choose pairs that  would  provide maxi-mum and stable yield and product quality, depending on the purpose of the variety. In the work various principles of the selection of pairs were used: ecological-geographical, according to the components of attributes  and selection of the maternal form. Using these methods, more than 300 combinations of crosses were obtained.  It is shown that the combination of two ecologically  different parental components of different origin - the local sample and the introduced one - leads to an increase in the heterosis  effect. The best hybrid combinations  with a complex of economically  valuable traits are obtained by crossing local varieties with selected breeding lines. When selecting pairs according to the components of the signs, where local or introduced samples with a different set of features were taken as parental forms, productivity was at the level of the standard. In most cases, when used as the maternal  form of the local variety, the productivity  of the hybrids was  significantly higher compared to  the  reverse crossing. As a result of our studies, it is once again confirmed and proved that the more differences in the places of cultivation and reproduction of parental components, the higher the heterosis effect and the effect of the maternal component is much higher.

Relevance. Pepper is a common crop both for fresh consumption and for the preparation of spices. Recently, along with the increasing popularity of C. annuum L. pepper, there is increasing interest in other species of this genus, which have a number of breeding and important properties. The most important method of enriching the gene pool of cultivated plants is distant hybridization, through which valuable traits are transferred from wild species to cultivated ones. The development of a new variety is a lengthy process, stretching over several years. In this regard, breeders have faced the challenge of obtaining pure lines to create a pepper hybrid with desired properties by applying modern biotechnological methods that will accelerate this process. One of them is the method of microspore culture, which allows mass production of haploid plants, reducing the time for creating constant parental lines.Material and methods. The aim of the work was to create an interspecific hybrid of hot pepper (C. annuum L. x C. frutescens L.) with high ornamental properties, a complex of economically valuable traits, with good taste qualities. The research was carried out in the film greenhouse of FSBSI FSVC in the Moscow region. The research material was a variety population of hot pepper Capsicum frutescens Cz-544-14, used as a paternal line, which was heterogeneous, and a pure line of C. annuum L. (Pb-551) created by classical breeding.Results. The pepper hybrid F1 Christmas bouquet was created as a result of hybridization of species parental forms obtained by different methods (biotechnological and classical). To accelerate the production of an aligned paternal form of C. frutescens L., the technology of doubled haploids through microspore culture was used. As a result, doubled haploid plants meeting the planned model (compact low habit, purple fruit colouring in technical ripeness and red in biological ripeness) were obtained. The resulting hybrid combined all the necessary economic features: high ornamentality, compactness, bouquet arrangement of fruits, high taste and aroma. Thus, the use of remote interspecific hybridization in the breeding process in combination with biotechnological approaches can accelerate the production of new forms of hot peppers that meet the demands of the market.

The important stage of breeding program for F1 hybrids with high yield capacity is to estimate the combining ability of parental lines for productivity characteristics. The information on combining ability may predict the productivity traits to exhibit and helps produce heterotic hybrids and use the initial breeding accessions in sweet pepper. The combining ability of breeding accessions for yield traits was studied with the use of complete diallel crossing. The research was carried out at experimental plots of State Institution ‘Transnistrian Institute of Agriculture’ in 2015-2016. In 2015, seven fertile lines were crossed through complete diallel cross to study combining ability. In 2016 the parental lines and 42 hybrids F1 forms produced from crossing were tested in open field condition. Analysis of General Combining Ability and Specific Combining Ability showed that none of all parental components had any essential expected effect for all tested traits of productivity. The line 134 showed the high effects of General Combining Ability as maternal and parental components for four traits, such as early and general yield, average weight and thickness of fruit pericarp. Out of 42 hybrid combinations studied, some accessions have been selected. They had the high effects of General Combining Ability with high constants of Specific Combining Ability for early yield – 4; general – 12; average fruit weight – 9; thickness of fruit pericarp – 4; dry matter content – 10; ascorbic acid – 13.

«The number  of infertile nodes»  is a stable  variety trait of pea plants, and it is directly related  to the duration  of the growing season.  To study the manifestations  of this trait in hybrid proge, nies the reciprocal  crosses among  pea samples  of  different groups of maturity have been made.

Relevance. The technology of producing cabbage seeds with the traditional method takes almost 2 years. Its high labor intensity caused the need to search for cheaper methods of selection and seed production. One of the ways to speed up the stages of the selection process is the use of artificial climate chambers. The aim of research is to obtain seeds of promising inbred lines of late maturing white cabbage and hybrid combinations with their participation using rosette plants (steckling) in artificial climate chambers. Materials and Methods. The material was inbred pure lines of 5-6 generations of inbreeding, obtained from promising hybrid combinations of domestic and foreign breeding. The research was conducted in 2023-2024. Sowing in Plantek 64 cassettes was carried out on July 10, then the seedlings were transplanted into vegetative vessels with an optimal volume of 5-6 liters and installed in the open ground for the growth of a leaf outlet. In the phase 10-13 of real leaves, the plugs were placed in an artificial climate chamber. During the growth and development period, biometric and phenological observations were carried out, and an analysis of seed setting was carried out during the hybridization of inbred lines.Results. As a result of research in the study of hybrid seed production under artificial climate chamber conditions, high rates of seed setting in buds under cross-pollination of rosette plants of white cabbage were revealed. Genotypes with high seed set rate under geitenogamous bud pollination were also identified. The weight of 1000 seeds of the studied plants was within the range of 2.5-5.14 g. Genotypes 383-3, 384-2 and 384-7 were selected, which had the highest percentage of seeds in the fractional composition with size more than 2 mm 80.3%, 43.5% and 59.4%, respectively. When genotypes 384-2 and 384-7 were used in hybrid combinations as a maternal component, high quality indicators of the obtained seeds were revealed.Conclusion. For the studied genotypes of white cabbage, when grown in an artificial climate chamber, no significant effect on seed quality was found; the genotype had a greater effect on seed quality indicators.

The article presents a brief history of the establishment of the Laboratory of Plant Breeding and Seed Production of Solanaceae Crops of FSBSI FSVC. The process of development of tomato, pepper, eggplant and physalis breeding is shown from the beginning of the establishment until the present time. Practical selection of Solanaceae crops in FSBSI FSVC is directly connected with the name of Academician VASKhNIL A.V. Alpatyev, who is the founder of scientific research on thermophilic crops. On the basis of conducted scientific researches the technique of creation of cold-resistant, early-ripening cultivars of tomato was developed, including influence of certain conditions on parental forms and hybrid material from its first generations to increase adaptability to new, not characteristic conditions of selected material. Subsequently, this technique was applied to the breeding of sweet pepper, eggplant and physalis. Interspecific hybridisation was used to enrich the gene pool of Solanaceous crops. As a result of distant hybridization, the following cultivars were created: the original tomato cultivar Severyanin with large parthenocarpic fruits on 1 and 2 racemes; an anadrous mutant for use in breeding; ultra-rapid, short-stage forms for obtaining heterosis effect; the cultivar Vkusny with increased content of dry matter in fruits. On the basis of interspecific hybrid of Ph. ixocarpa x Ph. angulata, the cultivars Dessert and Lakomka, characterized by high yield and resistance to diseases, high content of sugars, pectin and absence of bitterness, are created. Types of pepper Capsicum chinense, C. frutescens and C. annuum were used to create resistant forms to viral diseases. Involvement of wild eggplant species (Solanum aethiopicum, S. macrocarpon) to the breeding process allowed to obtain hybrid combinations with flavonoids content 1,3 times higher and phenolcarboxylic acids 1,6–1,7 times higher than in S. melongena.The development of theoretical bases ofSolanaceae crops breeding for resistance to biotic and abiotic stressors, as well as for high quality production, allowed to create a series of cosmopolitan cultivars, which at present are in demand, both in production and as a source material. The current scientific priorities are to use innovative biotechnology and molecular marking methods to accelerate the breeding process and improve selection efficiency.

The article deals with the stages of obtaining heterosis hybrids F1 watermelon. To solve the problem of creating heterosis hybrids watermelon allocated parent line with nuclear (gene) male sterility, which has a marker recessive trait – a single leaf blade. On the basis of this line, we create F1 watermelon hybrids that meet the requirements of modern production. The characteristic of the maternal line (line HMS) having sterile male flowers (gene ms) and wholeness (nl), light green color of the fruit (g), which is also a recessive trait. Also, the assessment on complex resistance to Fusarium and Anthracnose in linear hybrids F1 watermelon showed their superiority over the parent lines and a significant excess of the standard. Determined the combining ability of hybrid combinations of watermelon were evaluated for yield. According to the results of the evaluation of the ability to combine, a positive effect on productivity showed a combination with the paternal lines: Uspeh, Nice, Medunok, Vostorg, Favorite, Stimul, Icar, Rubyn, Pamyat Kholodova. The effect of more than 20 t / ha showed paternal lines Pamyat Kholoova, Favorite, Ikar. The optimal variant of the parent forms ratio is o: m: o: m: o (1:2), this scheme provides a higher hybridity of the obtained seeds. Hybridity of the obtained seeds was determined in the nurseries of preliminary and competitive variety testing. Hybridity of seeds was from 84.2 to 96.3%, which is satisfactory for industrial crops, where non-hybrid plants will be removed at breakthrough on the marker basis. Separated hybrids Line Cms x Rubyn, Line Cms x Medunok, the Line Cms x Favorite, Line Cms x Vostorg, Line Cms x Icar. The aim of the research was to develop the stages of obtaining heterosis hybrids F1 watermelon.