Relevance. The article highlights the issues of growing medicinal and essential oil crops, the seeds shortage and the problem of chemical polymorphism of raw materials. As a possible way to solve this problem is considered a wide introduction of seedling technologies for medicinal and essential oil crops. An analysis was carried out and information was systematized on individual elements of the technological process.Purpose. To analyze and explore the possibilities for the introduction of seedling technologies in medicinal plant growing, taking into account the experience at other branches of plant growing, primarily vegetable growing.Methods. Information obtained from literary sources was systematized by individual elements of the technological process, presented in a logical sequence and analyzed for applicability in medicinal plant production.Results. The result of the literary sources analysis, the personal experience of researchers on the use of the seedling method for medicinal, and essential oil crops growing, they showed the prospect the seedlings technology for generatively and vegetatively propagated plants. Thanks to modern growth regulators and fertilizers, it is possible to intensify the process of seedling production, and specifically to increase the germination and energy of seed germination, rooting of cuttings, shorten the period from sowing (planting cuttings) to planting in the ground and get cassette seedlings with a well-developed root system. The mechanization of the sowing and planting processes can significantly reduce the costs of these operations, a longer interval for planting allows you to prepare the field with the highest quality and successfully fight weeds by mechanical means. The technique used for these operations in commercial vegetable growing is suitable for medicinal and essential oil crops without further modification. Conclusion. The widespread introduction of the seedling method can be the best solution to the problem of lack of seeds, difficulties of direct sowing in the ground and a long germination phase, care after planting and weed control in the first period, and will also allow obtaining raw materials with a stable biochemical composition.

Relevance. Rhodiola rosea L. is a perennial herbaceous plant from the Crassulaceae family. The roots and rhizomes of R. rosea contain a range of biologically active compounds. This species is included in the State Pharmacopoeia of the Russian Federation. Medicinal preparations derived from them are widely used as adaptogens, general tonic and anti-stress agents. While the roots have been extensively studied, less attention has been given to the biologically active compounds found in the leaves of R. rosea. An introduced population of R. rosea grows on the territory of the botanical garden of medicinal plants of the All-Russian scientific research Institute of medicinal and aromatic plants (VILAR). The possibility of cultivating this species in the conditions of the Moscow region is being studied.Material and Methodology. The objective of this study was to investigate the composition of phenolic compounds in R. rosea leaves from VILAR biocollection using ultra-efficient liquid chromatography combined with mass spectrometry. Extraction was carried out with 80% acetone. An ultra-high performance liquid chromatographic system with a photodiode array detector and mass spectrometer was used for the analysis of phenolic compounds. Identification was carried out using UV- and mass spectrometry data.Results. As a result of UPLC-DD-MS analysis, peaks of 20 phenolic compounds were detected in R. rosea leaves, of which 19 compounds were tentatively identified. The leaf extract contained eight phenolic compounds from the group of galloyl-glucoses (three isomers of monogalloyl-glucose, trigalloyl-glucose, two isomers of tetragalloyl-glucose, two isomers of pentagalloyl-glucose), two gallotannins (isomers of hexagalloyl-glucose), five flavonoids (four herbacetin derivatives and one gossypetin), phenolic acids (caffeoyl-malic and ferulic acids), arbutin and esculetin. It has been shown that the biologically active substances of R. rosea provide prospects for the use of leaves as raw materials for the development of herbal preparations with anti-inflammatory, antiviral and antitumor effects.

Tanacetum balsamita L. is a perennial rhizomatous plant of the Asteraceae family. T. balsamita is cultivated as medicinal, aromatic plants and food plant. Extract of costmary exhibits hepatoprotective, antiseptic and anthelmintic properties. The herb of T. balsamita contains essential oils and phenolic compounds. This species included in the "Biocollection of medicinal and aromatic plants of open and protected ground", Botanical garden of the VILAR. The main aim was to study the composition of phenolic compounds in the leaves of T. balsamita with application of ultra-performance liquid chromatography coupled with diode array detection and mass spectrometry. The object of the study was the leaves of T. balsamita. Samples were extracted with 1 ml of 80% aqueous acetone. An ultra-performance liquid chromatographic system with a photodiode detector and a triple quadrupole mass spectrometer was used for analysis of phenolic compounds. The UV and MS data of phenolic compounds were used for their identification or tentative characterization with application of mass spectrometry databases and data published in the literature.Results. The results obtained showed the presence in the leaves of 17 phenolic compounds. Five compounds were identified as caffeoylquinic, feruloylquinic and three dicaffeolquinic acid isomers, and two compounds as ferulic acid hexoside isomers. The leaves of T. balsamita contained also five luteolin derivatives, two chrysoeriol derivatives, and spinacetin/axillarin. Four compounds were not identified.

Relevance.Meeting the needs of the pharmaceutical industry with domestic medicinal raw materials is relevant. Methods.The aim of the study is to determine the effectiveness application by growth regulator, organomineral and microfertilizers on Sage (Salvia officinalis L.) and Greater Burdock (Arctium lappa L.) in conditions of Non-chernozem zone of the Russian Federation. The experimental part of the work was performed in Institute of Medicinal and Aromatic Plants in 2010-2011 and 2016-2017 by field experiments. On burdock we tested fertilizer Siliplant (0.5 l / ha): the first treatment was in the rosette phase, the second - after 20 days. On sage studied binary mixture of organomineral fertilizer EcoFys (1 l / ha) with fertilizer Zitovit (0.5 l / ha; applied in phase regrowth of plants) with following foliar application by complex microfertilizers Siliplant (0.5 l / ha) and growth regulator Zircon (40 ml / ha). The solution consumption is 300 l / ha.Results. The effectiveness of chelated form micro-fertilizers and organomineral fertilizers applied on various medicinal plants was shown. It has been established, that complex application by EcoFys+Zitovit (I treatment) and microfertilizer Siliplant with growth regulator Zircon (II treatment) can increase the productivity of Sage:  yield of grass raised by 23%, roots by 40%. Similar results were obtained on the Greater Burdock culture – treatment of plants with microfertilizer Siliplant allowed to increase the yield of roots by 28%, leaves-by 24%.

Relevance. Currently pharmaceutical industry, food industry and chemical laboratories demonstrate an increasing interest in natural plant sources, including Chamaenerion angustifolium. Modern researches reveal more and more medicinal properties of this plant. For this reason, the demand for the use of fireweed as a medicinal plant, especially its leaves and flowers, is very high. It means, that the study of biological properties, chemical composition, ecological and landscape features of fireweed is of scientific and practical interest.Methods. This article contains information from literary sources about pharmaceutical, chemical, biological, ecological and geographical studies of Chamaenerion, its results and recommendations. Obtained from other researches information was systematized by research topics and presented in a logical sequence.Results. The issues of using this plant in medicine and food industry are also considered here, along with the discription of collecting natural fireweed problems and the mail ways of cultivating this species. The modern condition of fireweed research is shown, a detailed analysis of fireweed biological and ecological characteristics is carried out and on this basis, the main directions for further work on the introduction of Chamaenerion into culture for its more complete and rational use in medicine and in the food industry are outlined in this article. The article presents an analysis of the problems that arise during the introduction, in particular, the improvement of vegetative reproduction techniques, the selection of populations promising in terms of the phenolic compounds content and the possibility of target compounds content in plants by the exogenous regulation.Conclusion. Despite the presence of a relatively large quantity of works devoted to Chamaenerion, some research topics are poorly covered. For example, the topic of cultivation and the use of growth regulators. The most promising directions for the future study of Chamaenerion are indicated in this article.

Relevance. Taking into account the narrow range of vegetable plant species grown in Russia and the global trend of impoverishment of their biochemical composition, simultaneously with the globalization of crop production, it is necessary to search for plant species and forms that are characterized by a high content of functional food ingredients (FFI) in their composition, since a decrease in the consumption of important micro-and macroelements sharply increases the risk of dangerous diseases for humans.Objective. To study the features of growth and development of plants of the Solanaceae family in the collection of the Federal State Funded Institution of Science "NBG-NSC", characterized by a high content of biologically active substances and functional food ingredients (FFI), a short growing season and a neutral reaction to the length of the day.Materials and methods. Features of development, biomorphological indicators of plants were studied according to the method of introduction studies developed in the Nikitsky Botanical Gardens [4]. The antioxidant activity (AOA) was determined by the method [5] based on the redox reaction of substances of antioxidant action with potassium permanganate. The content of water-soluble minerals was determined using a TDS 3 conductometer on aqueous plant extracts. The content of phenolic compounds was determined by photometric method using the Folin-Chocalteu reagent [6]; ascorbic acid content - by iodometric titration [7], carotenoids content - by spectrophotometric method [8].Results. The revealed features of growth and development, biochemical composition of a new vegetable crop for the Russian Federation – nightshade caripense (tzimbalo) (Solanum caripense Dunal.), bladder herb (Physalis alkekengi L.), medicinal crops - nightshade laciniate (Solanum laciniatum Ait.), vitania hypnotic (synonyms winter cherry, Indian ginseng or physalis sunny-leaved) (Withania somnifera (L.) Dunal.), great morel (Atropa belladonna L.).Conclusions. The conducted research will allow expanding and replenishing the range of new crops with an increased content of biologically active substances and antioxidants.

Relevance. Meeting the needs of the pharmaceutical industry with essential oil raw materials and natural essential oils is relevant.Methods. The aim of the study is to determine the effectiveness the complex application by organomineral fertilizer EcoFys and the growth regulator Zircon on sage (Salvia officinalis L.) in conditions of Non-chernozem zone of the Russian Federation. The experimental part of the work was performed in Institute of Medicinal and Aromatic Plants in 2014-2015 by field experiments.Results. It has been established, that integrated application EcoFys and Zircon can increase yield of culture. In addition, it is shown that contain of essential oil in medicinal raw don’t increase and raising collection of essential oil provides only due to higher yield of herb.