The results of estimation of growth and development parameters, SSR analysis, electron microscopic examination of the surface of pollen and seeds, biochemical composition (ascorbic acid, carotenoids, carotenes, macro- and microelements content in the stem part of plant) of 8 Chinese broccoli, Brassica oleracea var. alboglabra samples grown in the unheated greenhouse of the Central Siberian Botanical Garden SB RAS, Novosibirsk (54°49′33″ N, 83°06′34″ E) and phy- totron of N. I. Vavilov Institute of Plant Genetic Resources (VIR) have been presented. Through the study, the large surface cellularity of B.oleracea var. alboglabra (cv. “Siji Xianggu jie lan” [“SX”]) seeds was similar with seed coat surface of B.oleraceae var. capitata f. alba cv. "Slava". A scanning electron microscopic study of Chinese broccoli pollen (cv. “SX”) revealed the formation of colpate pollen with ellipsoidal shape with a size of 32.9 ± 0.76 x 17.8 ± 0.42 μm, characterized by foveolate ornamentation with a length-to-diameter ratio – 1.85.The duration of the period from emergence stage to thickening of a stalk and flower-bud formation of cv. “SX” continued for 49-54 days. Сlose correlation was established between the largest diameter of the stem and the length of the leaf petiole (r=0,87, p < 0,001) and between the length of the leaf petiole and the weight of the stem (r=0,77, p < 0,001). As a result of molecular screening of Chinese broccoli using 5 SSR markers (Na10D09, Ol12F02, Ra2E12, BC 7 и BC 65) the greatest polymorphism was in the test with the BC 7 marker - five alleles from 160 to 295 bp in size. In the “SX” variety single flowering (10%) occurred on 59 ... 63 day and mass flowering (75%) – on 65 ... 68 day. The stem part of B. oleracea var. alboglabra is a fairly good source of vitamin C, accumulating as much as 32-46 mg/100 g, and also increased concentration of K, Ca, Mg and Cu. At the end of the season the fruits, together with the phytomass of stems are well-seasoned for 3-4 weeks forming viable seeds with germination above 85% corresponding to the Russian state standard (GOST 32592-2013) for white cabbage seeds.

Анализ изображений является доступным методом преобразования качественных переменных в количественные. Рассмотрена разработка в области компьютерного анализа изображений, которая улучшает понимание морфологии семян с точки зрения параметров их радиальной разнокачественности: размера, формы и цветовой гаммы. Цель работы - измерение геометрических показателей и анализа цветовых признаков семянок лука Кристофа (Allium cristophii Trautv.) в цветовом пространстве RGB, и выявление особенностей, обусловленных разноярусным расположением в соцветии. Проанализированы разнокачественные семена A. cristophii из биоколлекции Всероссийского НИИ овощеводства. Измерение морфометрических и оптических параметров семян осуществляли путем анализа их изображений с помощью программного обеспечения "ВидеоТест-Морфология". Проведенный анализ показал, что длина и ширина семянок с нижнего яруса составили 3,301 и 2,681 мм, со среднего – 3,295 и 2,605 мм и с верхнего яруса – 3,265 и 2,58 мм соответственно. Средний размер семянок с нижнего яруса находился на уровне 2,99 мм, среднего – 2,95 мм и нижнего яруса – 2,92 мм. Выявлено статистически значимое снижение показателей по всем цветовым каналам (RGB) от нижнего яруса к верхнему. Ярусное расположение цветков на соцветии является причиной неодновременного созревания семянок Allium. Оперативная легкость, низкая стоимость коммерческих компьютерных технологий, неразрушающий анализ семян и сортировка подчеркивают потенциал этого метода для применения в семенных лабораториях. | An image analysis is an accessible method of transforming qualitative variables to quantitative variables. There has been examined the development in the field of computer image analysis that improves the understanding of seed morphology in terms of their radial heterogeneity parameters: size, shape and color range. The aim of the work was to measure geometric indicators and to analyze the color characteristics of Persian onion (Allium cristophii Trautv.) seeds in the RGB color space, and to reveal the peculiarities determined by the multi-tiered arrangement in the inflorescence. The heterogeneous seeds A. cristophii from All-Russian Scientific Research Institute of Vegetable Growing biocollection of the Federal Scientific Vegetable Center were analyzed. The morphometric and optical parameters of the seeds were measured by analyzing their images using the VideoTesT-Morphology software. The analysis as carried out showed that the length and width of the seeds from the lower tier were 3.301 and 2.681 mm, from the average – 3.295 and 2.605 mm and from the upper tier – 3.265 and 2.58 mm respectively. The average seed size from the lower tier was 2.99 mm, the average size was 2.95 mm and the lower tier was 2.92 mm. A statistically significant decrease in the indicators over all color channels (RGB) from the lower tier – to the upper tier was revealed. The tiered arrangement of flowers on the inflorescence is the cause of non-time maturation of Allium seeds. Operational ease, low cost commercial computer technology, non-destructive seed analysis and sorting, highlight the potential of this method for application in a seed laboratory.