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Priming – innovative development of methodology preparation of seeds for sowing (review)
2023
A. V. Yanchenko | A. F. Bukharov | A. Y. Fedosov
The development of classical methods of soaking and growing seeds to the method of hydropriming and its further improvement based on the introduction of new knowledge and integrated approaches are shown. Priming is a controlled hydration of seeds (saturation with a limited amount of water), which causes the activation of metabolism, the initiation of germination (preventing the appearance of a root) and ensuring the subsequent reversibility of this process (by drying) while maintaining a stimulating effect. P.A. Genkel, who presented a thorough theoretical justification and detailed experimental confirmation of the effectiveness of pre-sowing soaking and subsequent drying (often multiple times) to increase drought and salt tolerance of plants. For a complex of methods for influencing seeds with water and osmotic solutions, W. Heidecker proposed the term priming, which is widely used in the English language, but at first did not take root in Russian literature. The characteristic of the internal reasons for the decrease in the viability of seeds, associated primarily with the aging process, during which the depletion of nutrient reserves, the accumulation of growth inhibitors, mutagens, reactive oxygen species and other harmful substances, the denaturation of proteins and nucleic acids, the oxidation of unsaturated lipids and external (abiotic and biotic), and their interactions. The article provides information about agents and methods, technical solutions and priming techniques. The role of water, conditions, factors, parameters, fundamental morpho-anatomical, physiological and biochemical substantiation of the germination process are described. The shortcomings of priming and ways to overcome them are outlined on the basis of an in-depth study of the metabolic processes occurring in the process of priming and subsequent germination in order to understand the mechanisms for increasing the sowing and productive properties of seeds. The necessity of maximizing the practice of applying priming, especially in difficult environmental conditions, was noted.
Show more [+] Less [-]Dynamics of changes in the content of betalain pigments in red beet roots during the growing season and storage
2023
V. M. Koldaev
Purpose. Betalains, plant pigments of red beet roots, exhibit antioxidant activity and reduce the risks of many pathological conditions. However, the widespread introduction of betalains is hampered by insufficient knowledge of their transformations during the growing season and storage of root crops, which was the purpose of the work.Methods. Root crops of five varieties of table beets were used in the studies. The content and stability of betalains were determined by spectrophotometric methods according to the numerical indices of the absorption spectra of extracts from root crops.Results. In the beet roots in the first 20 days of vegetation after germination, the content of betaxanthines is higher than that of betacyanins, but by the 40th day, betacyanins exceed betacyanins over betaxanthin in the ratio of 1.26-2.21. By the 70th 90th days of vegetation, the main pool of betalains is formed, their content reaches 84.5-198.6 mg / 100 g, the ratio of betacyanins / betaxanthins and resistance are 2.47-9.76 and 0.82-0.91 respectively. The highest excess of the content of betacyanins over betaxanthins by 8.11 9.65 times was obtained in beet root crops of Creolka and Veselaia Smulyanka varieties. The stability of betalains during six-month storage decreases less than 1.4 times.Conclusion. It is advisable to use the developed spectrophotometric method for determining the stability of betalains in the express analysis of beet root crops. Betalains are more stable than other plant antioxidants. Beet roots are more preferred for fortifying diets than other foods with antioxidant activity.
Show more [+] Less [-]Immunomodulatory properties of plants of the genus Filipendula
2023
E. V. Sokolova | D. N. Baleev
This paper analyzes the literature data on the practical use of plants of the genus Filipendula as a nutritional component and a promising source of biologically active substances. For immunomodulatory action, the flowers and leaves of the meadowsweet are mainly used, which have demonstrated a significant effect in numerous in vitro and in vivo models. Among the diseases in which the immunomodulatory properties of plants of the genus Filipendula are found the most application are inflammatory processes of the upper respiratory tract, a number of inflammatory disorders of the skin, as well as a diaphoretic and antispasmodic, in bronchial asthma, etc. An analysis of the literature data allows us to conclude that these plants have a beneficial effect on human health, both individually and as a component of food, due to their unique phytochemical profiles.
Show more [+] Less [-]Evaluation of green beans (Phaseolus vulgaris) varieties for adaptability and noble-forming ability in the conditions of the forest-steppe of Western Siberia
2023
O. E. Yakubenko | O. V. Parkina | Zh. Wang | N.T. Nguyen
Relevance. From an agrotechnical point of view, beans are a valuable crop capable of greening agriculture. Legumes have the ability to enter into symbiotic relationships with nodule nitrogen-fixing bacteria and assimilate atmospheric nitrogen. The creation of highly efficient plant-microbial systems in agrocenoses by breeding new varieties of legumes with a high symbiotic potential is a promising direction that makes it possible to control the efficiency of plant biomass accumulation and the quality of agricultural products. There is a need to evaluate varieties for adaptability and nodule-forming ability to implement breeding programs to create highly productive and high-quality varieties that can realize the genetic potential of productivity in the sharply continental climate.Materials and methods. On the basis of the Federal State Budgetary Educational Institution of Higher Education Novosibirsk State Agrarian University in 2018-2020, an assessment was made of green bean varieties of different ecological and geographical origin with a bush type of growth. Experimental fields are located in the village of Michurinsky (left bank of Novosibirsk) and the educational and production farm "Garden of Michurintsev" (right bank of Novosibirsk). The study of samples in terms of adaptability parameters, as well as the ability to form nodules, was performed in accordance with generally accepted methods. We took into account the characteristics that affect the productivity of the crop - the number and weight of beans per plant, the weight of one bean, and the yield. The aim of the work is to evaluate the varieties of vegetable beans for adaptability and nodule-forming ability in the conditions of the forest-steppe of the Siberian region.Results. When forming the symbiotic apparatus, the growth conditions were taken into account. It has been established that high rates of biomass are observed on the territory of the village of Michurinsky. Varieties Morena and Maguyra are characterized by intensive growth in the experimental field of the village of Michurinsky, varieties Zoluyshka and Solnyshko – the educational and production farm "Garden of Michurintsev". The total number of nodules per plant varied from 29 (Kormilitsa) to 66. (Zoluyshka), active nodules – from 9 (Kormilitsa) to 49 (Zoluyshka). It is noted that the varieties Solnyshko and Zoluyshka have high rates of general adaptive ability and breeding value of the genotype. A strong relationship has been established between the characteristics of leaf mass and the number of active nodules, the number of active nodules and the leaf surface area; average - between the number of active nodules and plant biomass.
Show more [+] Less [-]The effectiveness of the complex application of humates and micronutrient fertilizers on the <i>Macleaya x kevensis</i> Turill. and <i>Macleaya cordata</i> (Willd.) R.Br.
2023
O. A. Bykova | R. N. Thaganov | V. R. Thaganov | A. I. Morosov
Relevance. In the arsenal of agents with antibacterial activity, a significant place belongs to preparations based on the raw materials of two types of Macleia - Macleaya x kevensis Turill. and Macleaya cordata (Willd.) R.Br. used both in medical and veterinary practice. It is possible to provide the domestic pharmaceutical industry with these medicinal raw materials in full through the development of methods for increasing yields and adapting Maclea to unstable environmental weather conditions.Material and methods. Experiments to study the effect of the use of humates and microfertilizers on these indicators were laid in the fields of the North Caucasus branch of VILAR in 2019-2021. We studied the morphological and economically valuable traits of two species of Maclea, evaluated the growth rates of plants, the yield of raw materials, the content of alkaloids and their collection per hectare under optimal and dry weather conditions.Results. Differences between the two types of Maclea are established in terms of morphological and economically valuable traits. Maclea heart-shaped palmate-lobed leaves are light green in color, the roots are vertical, round-cylindrical, reaching a depth of 50 cm or more, plant height is 300-350 cm. at a depth of 25-30 cm, plant height 200-250 cm. % versus 0.162-0.164% for the heart-shaped Maclea. The dependence of the growth, development and productivity of two types of Maclea on meteorological conditions has been established. Under the conditions of insufficient water supply and high temperatures, the loss of yield in the first year of vegetation in Macleaya cordata is 14%, in the second year - 6%, in Macleaya x kevensis - 21 and 12%, respectively. Foliar treatments with the Normat L complex with Ferovit in the first year of vegetation contributed to a decrease in crop losses and an increase in the yield of alkaloids per 1 hectare in both types of makley, in the second year, with these treatments, an increase in yield by 7-11% was observed, the collection of alkaloids per hectare increased by 14- 18%.
Show more [+] Less [-]Assessment of the effect of a complex of biological products on the growth, development and yield of cucumber in light culture
2023
M. A. Bocharova | V. I. Terekhova | T. S. Aniskina
The ecologization of protected soil is currently one of the most important tasks of agricultural production, in this regard, for the vegetable growing of protected soil, the introduction of effective microorganisms into the substrate when growing plants in low-volume technology is of great interest. Biologics can enhance metabolic processes in the nutrient substrate and plants, plant resistance to adverse environmental conditions, increase plant productivity and improve product quality. Therefore, the purpose of our work is to analyze the effect of a complex of biological products on the agrobiological parameters of cucumber hybrids Mewa F1 and Valigora F1 on the 39, 67, 95 and 123 day of cultivation from the appearance of mass shoots. The research was carried out in conditions of high industrial greenhouses of the "Venlo" type, in the winter-spring turns of 2021 and 2022. The preparations were introduced into the tank mixture and fed to the plants through a drip irrigation system. Measurements of economically valuable indicators were noted weekly. Descriptive statistics and ANOVA methods were used for statistical data analysis.Results. When using biological products, a positive effect was noted on the growth and development of cucumber plants, as well as on the increase in leaf plate and leaf surface area (LAI). The dates of the onset of single and mass flowering of plants and single fruiting occurred earlier when using a complex of biological preparations for 1-5 days. When assessing the effect of the complex of biological products on the development of the photosynthetic apparatus, the positive effect of biological products on the area and index of the leaf surface on the 39, 67 and 95 days of cultivation was established. In addition, a significant effect of the complex on the increase in yield per m2 per turnover was revealed. In the conducted studies, the increase in yield was due to an increase in the weight and diameter of fruits. The total height of the plants and the weekly growth of the complex of drugs practically did not affect. A comparative analysis of the growth processes and yield of cucumber hybrids Mewa F1 and Valigora F1 when applying root fertilizing with a complex of biological preparations showed great effectiveness from their use.
Show more [+] Less [-]Evaluation of mutagenic samples of winter garlic
2023
V. I. Nemtinov | Yu. N. Kostantchuk
Relevance. The use of chemical mutagenesis on garlic is relevant for the creation of new forms with stable characteristics, better biochemical qualities, increased yield, for use in breeding practice.Results. The reaction of garlic treated with two chemical mutagens was evaluated: DES (diethyl sulfate) – 0.025; 0.05 and 0.1% (mutants 2, 3 and 4), as well as DMS (dimethyl sulfate) – 0.02; 0.04 and 0.08% solutions (mutants 5, 6 and 7). The aftereffect of chemical mutagens was noted with low and medium variability of signs in the second–fifth generation. According to the low variability of 1.6–9.4%, the height of plants (samples M 3 and 6), the number of leaves (M 5), the height of the false stem (M 3), the diameter of bulbs (M 6), as well as the length of leaves and the weight of bulbs samples M 3, 5 and 6 were distinguished. The effect of mutagens on morphometric signs of garlic were also tracked in the second generation (re-growing from air bulbs), where the parameters of low and medium variability of signs are marked. The majority of samples on 7 signs reflected a low variability of 5.5–10.0% and only 2 mutants showed an average variability of 11.4–16.4%: M 6 in plant height and false stem and M 3 in bulb diameter and yield. The correlation between their value in the 5th generation and in the 2nd generation also revealed strong direct and average connections. High heritability of h2 signs = 0.74–0.99 for DES preparations – 0.05%, VMI – 0.02 and 0.04%, was the most important indicator of the selection of valuable signs.
Show more [+] Less [-]Efficiency of distributed application of nitrogen on onion (<i>Allium cepa</i> L.) cultivated as an annual crop
2023
I. I. Irkov | O. N. Uspenskaya | N. I. Bernaz
Relevance. Onions in Russia in 2021: Cultivation area – 56.3 thousand hectares; Productivity – 28.6 t/ha; Production – 1608.6 thousand tons; Import from the volume of consumption – 10.7%. Modern varieties and hybrids and plant protection products make it possible to obtain high yields in the conditions of the Non-chernozem zone. The aim of the research is to optimize the parameters and terms of technological operations for the cultivation of onion in an annual crop on alluvial meadow soils of the Non-Chernozem Zone.Materials and methods. The nutrition system of onion plants was tested through distributed nitrogen application during the growing season: Option 1 – (N160P160K160) presowing – (Ca40N10 + K40N20 + K40N20) during the growing season; Option 2 – (N110P110K110) presowing + (Ca40N20 + K40N40 + K40N40) during the growing season. Experience in the use of the microbiological preparation Bisolbi-Plant (Bacillus pumilis) BIS88 together with the 1% humic preparation "Rostok" at a rate of one liter per hectare each to stimulate active growth.Results. At the end of the growing season in the second variant of the nitrogen experiment, the growing season was extended by 7-10 days. The excess yield under the second option for 2021-22 years of research amounted to more than 20.0 t/ha and is significant. The difference in the options for applying the Bisolbi-Plant preparation in 2.8 t/ha with HCP05 – 8.2 t/ha was not significant. The advantage of the variant with the biological preparation is higher plant density by 71.0 thousand units/ha and the absence of signs of bacteriosis. Thus, the distributed application of nitrogen (N110P110K110) presowing + (Ca40N20 + K40N40 + K40N40) during the growing season with irrigation water was significantly more effective than (N160P160K160) presowing + (Ca40N20 + K40N20 + K40N20) during the growing season. There was a tendency to increase the yield and quality of products with the double application of Bisolbi-Plant (BIS88) at a rate of (1.0 + 1.0) l/ha.
Show more [+] Less [-]Selection and seed production of vegetable crops – on an innovative path of development
2023
A. V. Soldatenko | V. F. Pivovarov | O. N. Pyshnaya | L. K. Gurkina | E. V. Pinchuk
The vegetable growing industry in Russia is the main component of crop production, the development of which determines the basis of life and food security. Being the most important and indispensable products of plant origin in the human diet, for a Russian, vegetables are in third place in importance after bread and potatoes. However, according to Rosstat, the level of selfsufficiency in vegetables and food melons in the country is 86,3%, which implies the need to increase their production. To improve the situation in the vegetable growing industry, an important place is given to the issues of improving selection and seed production, since the variety and high-quality seeds are the main elements of modern zonal crop cultivation technologies. At the Federal Scientific Center for Vegetable Growing, selection and seed production of vegetable crops is moving to an innovative development path, where, along with classical methods, modern methods are used: molecular marking according to the main economic characteristics, accelerated creation of homozygous lines using doubled haploid methods, plant immunity. In recent years, various methods of pre-sowing preparation of seeds have been developed to increase their viability and germination friendliness, to obtain even seedlings without harming the ecosystem. Based on the fundamental and priority applied research of previous years, in 2022, the creation of 17 varieties and hybrids of vegetable, melon and flower crops for all potential vegetable growing zones was completed. In order to provide the industry with pure-grade high-quality seed and increase the productivity of crops through the full use of the potential of the variety, the Federal Research Center for Vegetable Growing is constantly working in primary seed production. The developed zonal cultivation technologies will allow growing domestic products in various soil and climatic conditions of the country.
Show more [+] Less [-]Impacts of climate change on vegetable production and ways to overcome them
2023
A. F. Bukharov | A. Yu. Fedosov | M. I. Ivanova
Vegetables play a key role in food security and nutrition as the food system shifts from food quantity to dietary quality and health benefits. One of the main reasons for the low production and declining average yields of most vegetable crops is climate change. Important limiting factors in maintaining and increasing vegetable crop yields are rising temperatures, reduced water availability for irrigation, flooding and salinity. Under changing climatic conditions, crop failures, declining quality and increasing pest and disease problems are becoming commonplace and making vegetable production unprofitable. Since many physiological processes and enzyme activity are temperature dependent, they will be greatly affected. Drought and salinity are two important effects of rising temperatures that reduce vegetable crop yields. These impacts of climate change also affect the emergence of pests and diseases, host-pathogen interactions, distribution and ecology of insects, timing of emergence, migration to new locations, and their ability to overwinter, all of which become a major barrier to vegetable production. To mitigate the adverse effects of climate change on the productivity and quality of vegetable crops, sound adaptation strategies need to be developed. Emphasis should be placed on the development of production systems that improve water efficiency and are adapted to hot and dry conditions. Technological practices, such as mulching with crop residues and plastic materials, help maintain soil moisture. Excessive soil moisture due to heavy rains becomes a serious problem that can be solved by growing crops in raised beds. An effective way to solve these problems is to create genotypes that are resistant to high temperatures, moisture, salinity and resistance to climatic conditions, using traditional and non-traditional breeding methods, genomics, biotechnology, etc.
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