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Rooting stimulation of “Victoria” and “Korinka russkaya” grape hardwood cuttings as influenced by potassium salt of Indolyl-3-Acetic Acid (KIAA)
2020
I. H. Shaimaa | A. A. Shalamova | A. G. Abramov
Relevance. Applying exogenous auxins to the cuttings of grapevines is a common practice in viticulture to improve the rooting process. The potassium salt formulations of auxins have been documented to be more, less, or equally efficient as acid formulations in rooting stimulation of cuttings depending on the genetic features of the plant, type and concentration of auxin, type of cuttings, and many other factors.Methods. The present study aimed at evaluating the effect of potassium salt of indolyl-3-acetic acid (KIAA) on rooting of the hardwood cuttings of two grape cultivars namely, Korinka russkaya and Victoria, which are commonly planted by grape growers of the Tatarstan Republic. The study was conducted in 2018 and 2019 under hydroponic conditions in a controlled-environment growth chamber in Kazan State Agrarian University. The cuttings were treated with 1) KIAA at two concentrations (5,000 and 10,000) ppm; 2) β-indolyl-butyric acid (IBA) 3,000 ppm (as a check treatment) and 3) distilled water as a control.Results. Based upon the overall results, KIAA significantly improved the rooting process of both investigated cultivars. Rooting percentage, average number of roots on cuttings as well as the quality of the root system was enhanced with increasing auxin concentration up to 10,000 ppm. IBA also improved the rooting quality parameters over the controls. The two grape cultivars differed significantly in their rooting capacity: cuttings of Korinka russkaya in both years had greater values of all the studied parameters compared with those of Victoria cultivar except for the callusing percentage, which was higher in Victoria.
Show more [+] Less [-]Determination of bean resistance to anthracnose pathogen – Colletotrichum lindemuthianum Br. et Cav
2020
A. M. Pashkevich | A. I. Tchaikovsky | N. V. Medved
Relevance. Currently, the range of vegetable crops has firmly entered the bean, the protein of which is absorbed by the human body by 87% or higher. The main factor limiting the cultivation of beans in Belarus is the lack of varieties of this crop that are resistant to mycological diseases, primarily Anthracnose.Materials and methods.The study included 34 samples of vegetable beans of mutant and hybrid origin obtained in the sector of legumes of vegetable crops of RUP "Institute of vegetable growing of the National Academy of Sciences of Belarus". To obtain a mutagenic effect, induced mutagenesis was used in the form of the action of a radioactive isotope 60Со and a neodymium laser. Hybrid combinations were obtained by intraspecific hybridization of mutant samples. For molecular genome testing, DNA was isolated from plant leaves before flowering, and its purification was performed by Fermentas using a Genomic DNA Purification Kit. The concentration of the resulting DNA solution was determined by a spectrophotometric method on a CARY 50 SCAN. A Thermo Hybaid RX2 amplifier was used for PCR. The annealing temperature of the primer was determined using the program "oligocalculator". The CV 2014 primer was used as a marker. resistant genotypes to Colletotrichum lindemuthianum Br. et Cav were at the band level of about 450 BP. Phytopathological assessment of plant infectivity was carried out on the basis of visual and instrumental methods, taking into account the reaction of plants and the intensity of the lesion, in the conditions of a natural infectious background in the phase of technical ripeness.Results. Seven samples of beans were identified that have genetic resistance to Anthracnose and show it in the conditions of a natural infectious background.
Show more [+] Less [-]Evaluation and characteristic of economically valuable signs of muscular and large-freeded portion size lines
2020
O. V. Yakimova | V. E. Lazko
Relevance. In the consumer market, there is a popular pumpkin in portion sizes weighing 1.0 to 2.5 kg. To meet the needs of the population and producers in pumpkin fruits, F1 varieties and hybrids with high economically valuable traits, portion size and manufacturability in production and processing are needed.Material and methods. The purpose of the research is to analyze, isolate and select the best breeding samples for creating parental lines of butternut pumpkin and large-fruited portion size.The experiment was carried out at the selection plot of the FSBSI "Federal Scientific Rice Centre" from 2015 to 2019. The objects of research were cultivars, hybrid populations, cultivars and samples obtained from different sources.Results. Lines with a number of economically valuable traits are highlighted, including portion size, productivity, biochemical parameters and commercial attractiveness of fruits. According to the length of the growing season, all inbred lines were divided into 3 groups: early maturing, mid-maturing, late maturing. In terms of productivity, the following lines stand out: KrChkh, BatS, La. The following lines were distinguished by the content of dry soluble substances (SRV, mg%): KrChkh, LaChkh, BatS, LaN. In muscat lines, the number of marketable fruits per plant varied from 3.3-5.7 pcs / plant. According to keeping quality, the test samples were divided into several groups: the least keeping quality among the studied samples was the line 15-5 (105 days); in fruits of lines 24-3, 16-5, 15-1, the safety of fruits increased to 116-126 days. The 28-2 line (162 days) was the highest keeping quality. The rest of the lines were attributed to medium bikes (133-148 days). All selected lines were characterized by a female type of flowering, multi-fruiting, short-leaved and good keeping quality of fruits. The KrChx line has a pronounced leaf aerenchyme. The organoleptic analysis showed that all the lines identified have a high score of 4.5-5. All commercially valuable traits in the lines are fixed with an inward.
Show more [+] Less [-]Biological features of the growth and development of varieties of a peach in the conditions of the seaside lowland of a flat zone of Dagestan
2020
A. V. Ryndin | N. G. Zagirov | N. A. Ibragimov
Relevance. The creation of the peach gene pool should be directed taking into account the specifics of the winter conditions of the subtropics of Russia.Methods. The aim of the research is to study the biological characteristics, phenological phases of peach varieties, their growth and development to optimize the distribution of plantings in industrial peach plantations in the conditions of South Dagestan. Long-term studies were carried out in 2015-2018 using generally accepted programs and classical methods of variety research and selection research.Results. The dates and durations of the passage of the main phenological phases of the development of introduced peach varieties are determined. Flower buds swell in 21 days, flower buds bloom in 7 days, flowering in 8 days, fruit ripen in 10 days. The expediency of peach cultivation in the southern lowland zone of the Republic of Dagestan is established. Resistance to various abiotic stress factors of the environment in modern conditions of climate change is determined. The relationship between the stages of the phenological phases and the calendar variability of these stages depending on the accumulation of the sum of effective temperatures are revealed; set biometric features of tree growth. This makes it possible to isolate varieties with moderate growth for compacted peach plantings in the conditions of Southern Dagestan.
Show more [+] Less [-]Protection from diseases of tomato cultivation in the open ground in the Primorsky Territory
2020
I. A. Vanyushkina | N. P. Kushnareva
Relevance. Climatic factors of Primorsky Region make the most favorable conditions for developing of a diseases of vegetable crops. In such conditions leaves surfaces of the plants are defeated with the phytopathogens from Phytophthora, Alternaria, Septoria and Xanthomonas genus.Materials and methods. In the years 2016, 2017 some research on determination of the effectiveness of protective activities for the diseases control of tomatoes in the open field were taken in the Primorskaya Vegetable experimental station. Some activity was carried out with the sort of tomatoes Odissey in the coastal zone of Primorsky Region in the conditions of the monsoon climate with annual rainfall 700-800 mm, greater part of which falls in the summer season. Fungicides Acrobat MT, Orvego, Cignum and Skor were chosen for studying. The first cultivation with preparations took place in the first decade of July in a period of appearing of the first signs of Alternaria on tomato leaves surfaces. Two next cultivations were carried out with the intervals of 14-16 days according to the weather conditions.Results. At protecting from Alternaria (79.4%) the greatest biological effectiveness was shown by the fungicide Cignum. The biggest tomato crop productivity was also reached thank to cultivation with this fungicide: the crop raising of tomato commercial fruit was 15.2 t/h or 70.4% by comparison with the control version.
Show more [+] Less [-]Nutritional value of salad Crimean onion
2020
N. A. Golubkina | V. I. Nemtinov | Yu. N. Kostanchuk | G. Caruso | A. F. Agafonov | I. S. Mastyaev | S. M. Nadezhkin
Relevance. Allium cepa L. is considered to be one of the most important agricultural crops in most countries of the world. High nutritional value is especially pronounced in salad onion which vegetation needs high temperature and significant insulation.Material and methods. The first biochemical characteristics are given for 9 Crimean salad onion genotypes and varieties (red onions Festivalny, Yaltinsky plus, Yaltinsky foros, Yaltinsky rybin, Yaltinsky 6А, Yaltinsky 7А, Yaltinsky 8А and yellow cultivars Kendy and Bean promine) with the comparison with the appropriate data for popular Italian sweet onion cultivar Tropea and semi-pungent varieties grown in Moscow region, Italy and Stavropol region (Zolotnichok, Cherny prince).Results. Warm climate is shown to increase the amount of antioxidants, monosaccharides and organic acids in onion bulbs. Compared to the Italian Tropea variety Crimean genotypes were characterized by higher levels of ascorbic acid (12.8±1.4 mg/100 g fresh weight) and organic acids (1.58±0.03 mg malic acid per g of dry weight), relatively similar levels of polyphenols (21.0±2.3 mg-eq Gallic acid per g of dry weight), and quercetine. (3.09±0.42 mg-eq Gallic acid per g of dry weight) and antocyanines (2.78±1.03 mg/g fresh weight). New genotypes Yaltinsky 6A, Yaltinsky 7A, Yaltinsky 8A were shown to accumulate higher levels of antioxidants. A direct correlation between the peel/inner layers ratio of antioxidant activities with the appropriate ratio for polyphenols was demonstrated (r=+0.93). A direct correlation between flavonoids content in peel and inner layers was also revealed (r=+0.83). Polyphenols and flavonoids ratios between peel and inner layers were shown to be higher for the Crimean genotypes compared to semi-pungent Moscow region varieties which indicates the prospects of utilization of the Crimean onion peel in production of functional food products with elevated levels of antioxidants.
Show more [+] Less [-]Antioxidant status of celery (Apium graveolens L.)
2020
V. A. Kharchenko | A. I. Moldovan | N. A. Golubkina | A. A. Koshevarov | G. Caruso
Relevance. Medicinal properties of celery (Apium graveolens L.) are connected to the most extent to high antioxidant status of plants.Material, methods and results. Comparative evaluation of polyphenols, ascorbic acid, flavonoids and photosynthetic pygments by leafy, stalk and root celery types, grown in similar conditions on experimental fields of Federal Scientific Center of Vegetable Production revealed several peculiarities, typical to each form. Antioxidant activity (AOA) of seeds, leaves and petioles composed a ratio: 2.7:1.74:1 for leafy form, 2.88:1.99:1 for stalk form, while root celery AOA of seeds, leaves, roots and petioles was characterized by a ratio 3.66:2.78:1.42:1. With practically no differences in leaves ascorbic acid content between different celery forms leaves:petioles ratio for ascorbic acid reached (5.31-5.57):1 for leafy and stalk forms whereas vitamin C distribution between leaves, roots and petioles of root celery was characterized by a ratio 8.1:1.1:1. Phenolics seeds:leaves:roots:petioles accumulation ratio for root celery was equal to 1.77: 1.77: 1.33:1, whereas leaves/petioles distribution of phenolicss in stalk andleafy forms reached 1.86:1 and 1.64:1 accordingly. Polyphenols content in seeds of leafy celery was 1.4-1.5 times lower than in leaves, contrary to stalk and root celery with equal concentrations of phenolics in seeds and leaves. Leaves/petioles ratio of flavonoids was the highest for Elixir (2.87) and Atlant culivars (2.41). Root and leafy celery Samuray cv demonstrated 1.54-1.71 appropriate values. The highest chlorophyll a and b content was typical for root celery. Celery antioxidant system was characterized by positive correlations between ascorbic acid, flavonoids, phenolics, zinc and total AOA and also between the AOA ratio seeds/petioles and leaves/petioles (r=+0.99; P<0.001) and a negative correlation between chlorophyll and phenolics and flavonoids accumulation levels. High nutritional significance of root celery leaves was estimated.
Show more [+] Less [-]Phytoncide action of essential oils of medicinal plants on the causative agents of broad beans alternariosis and cladosporiosis
2020
Yu. N. Kurkina
Relevance. The use of chemical fungicides in protecting plants from pathogens is the most common way, despite the proven danger of pesticides. The use of biofungicides based on essential oils of medicinal plants, which also have phytoncidal properties, can be a suitable alternative to chemical preparations. In the years favorable for the development of alternariosis or cladosporiosis, spots appear on the plants, leading to necrosis and significant loss of seed productivity. Therefore, the search for new effective and safe means of combating fungal phytopathogens becomes relevant.Methods. Alternaria alternata (2018-1.1) and Cladosporium cladosporioides (2019-11.4) strains were isolated from leaves with signs of alternariosis and cladosporiosis, on which the essential oils Abies alba, Artemisia vulgaris, Caryophyllus aromaticus, Coriandrum sativum, Lavandula officinalis, Ocimum basilicum, Pimpinella anisum, Rosmarinus officinalis, Salvia sclarea, Thymus vulgaris. The antifungal activity of essential oils was evaluated by comparing the diameter of the experimental (with the addition of essential oil to the Petri dish) and control colonies using the author’s scale.Results. All studied essential oils had antifungal properties, but species-specificity was observed. Essential oils of basil, coriander and thyme showed a fungicidal effect on C. cladosporioides and A. alternata. Lavender oil proved to be a fungicide for A. alternata and a strong fungistatic for C. cladosporioides. To create biological products for plant protection against alternariosis, sage essential oil (moderate fungistatic effect) may also be of interest, and cladosporiosis - anise and rosemary oils.
Show more [+] Less [-]Evaluation of the adaptive ability and stability of the Siberian bean vegetable gene pool
2020
O. E. Yakubenko | O. V. Parkina | K. I. Popova | D. A. Kolupaev
Relevance. The evaluation of adaptive ability of varieties is an actual direction in selection. Phenotypic plasticity of legume crops, in particular green bean, in Siberian region has not been studied enough. There is a need to evaluate grade samples for adaptability and stability, as highly and medium adaptive varieties can realize their genetic potential of productivity in extreme continental climate of Western Siberia.Materials and methods. On the basis Department of Selection, Genetics and Forestry, Novosibirsk State Agrarian University (2015-2019), long-term evaluation of grade samples of green bean of different ecological and geographical origin with a bush type of growth was carried out. Of particular interest are signs that have a significant impact on crop productivity. The signs were studied: number of beans on plant, weight of beans from plant, weight of one bean, yield capacity. The objective of the study is to evaluate the adaptive ability and stability of common bean varieties in the conditions of Siberian region.Results. The conditions of Western Siberia are suitable for cultivating vegetable beans on a green spatula and seeds. It has been established that, based on the weight of one bean, the coefficient of variation has a negative value. This indicates a low variability of the trait depending on the natural and climatic conditions, which allows selection of breeding valuable varieties for this parameter. As a result of the study, 6 genotypes of the crop, combining high productivity and environmental resistance, were selected. It was established that varieties Veronika and Darina can be referred to non-plastic samples. Samples could diversify breeding material of the crop and show the value according to specific features in edaphic-climatic conditions of Western Siberia.
Show more [+] Less [-]Assessment of significant for breeding morphological traits in the double haploid population of Brassica rapa L. in controlled conditions of a regulated agroecosystem
2020
K. V. Egorova | N. G. Sinyavina | A. A. Kochetov | Yu. V. Chesnokov
Relevance. The genus Brassica includes the most important vegetable, oilseed, spice and forage crops. Many representatives of cabbage are widely used as model objects for studying economically valuable traits. Limited publications devoted to the study of genetic aspects of the implementation of morphological quantitative features of B. rapaand the lack of data on the influence of environmental parameters on their implementation make it a very urgent task to study these features in the RAES. Material and methods.The purpose of this work is to study the influence of environmental light factors on the manifestation of economically valuable features in the mapping populations of B. rapain the RAES. The peculiarity of our research is the study of lines of mapping populations of B. rapa in strictly controlled conditions of the RAES. The studied populations were grown under optimal conditions with a 12-hour photoperiod. 21 DH 30 lines and 31 DH 38 lines were used. Several types of economically valuable traits were studied: the timing of transition to flowering, the growth rate of the plant, the habit and morphological characteristics of the leaf and leaf rosette. Results. A high degree of variation in the expression of the studied traits between lines within the population was revealed, which is consistent with previously published data [1, 2]. Highly productive genotypes, which can be used in further genetic selection work, including for obtaining genotypes, lines, and varieties adapted for growing in light culture, have been selected. The obtained data can also be used for further study of the influence of physical environmental factors on the manifestation of selectively significant quantitative traits in cabbage plants.
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