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REGENERATION OF WINTER GARLIC PLANTS (ALLIUM SATIVUM L.) IN VITRO FROM BULBILS
2018
A. V. Polyakov | M. A. Azopkova | N. N. Lebedeva | I. V. Muraveva
Garlic is exclusively vegetatively propagated plant which damaged by numerous virus, bacterial, fungus and other phytopathogens. Under propagation of damaged plants phytopathogens are transferred to progeny that leads to decrease in productivity, loss of quality, storability and frequently to degeneration of cultivars. In this regard improvement of landing material is obligate stage of the modern technologies of garlic production. Experimentally reasonable technology of the obtaining revitalized landing material of winter garlic based on in vitro cultivation of air bulbils on MS medium containing BA in concentration of 2 mg/l and NAA – 1 mg/l, which followed by formation of sprouts and then plants with bulbs is presented in the article. It is established that application of graduated sterilization of air bulbils isolated from not revealed inflorescences with diameter up to 25 mm for introduction in vitro culture allows to obtain plants free of internal infections. One of important factors influencing effectiveness of induction of morphogenesis is the plant genotype. Cultivar Emperor was characterized by high morphogenetic potential in vitro culture which significantly surpassed cultivar Gladiator at all stages of cultivation. The share of vital explants of Emperor cultivar consisted of 74,2%, and Gladiator cultivar – 56,3%. The morphogenesis of air bulbils in vitro conditions depends on their age. The share of vital micro bulbs with leaves formed under cultivation of air bulbils within 21 days isolated on 7th day after of exit of inflorescences from leaf sockets in depending on cultivar consisted of 65,0% and 76,4%. Cultivation of air bulbils of more old age is followed by their 100% growth with formation of leaves and microbulbs. Adaptation of such plants to ex vitro conditions is followed by obtaining monoclove bulbs cultivation of which in the open ground conditions leaded to formation of polyclove bulbs.Cultivation of plants in vitro on the medium containing sucrose in concentration of 10% gives the chance to keep viability material within 5-6 months.
Show more [+] Less [-]METHOD OF DETERMINING THE DYNAMICS OF INTERACTION LACTIC ACID BACTERIA IN THEIR JOINT CULTIVATION
2018
V. V. Kondratenko | N. E. Posokina | O. Yu. Lyalina | A. Yu. Gracheva | Zh. A. Semenova | V. I. Tereshonok
The processes of fermentation, accompanied by the growth and development of several species of microorganisms, are often accompanied by a change of some species of microorganisms for anothers , such a complex "behavior" of microorganisms during fermentation needs to be be deeply researched. The previously used approaches of evaluation of the type of interaction of particulate monocultures in consortiums make it possible to obtain only qualitative results (synergistic, antagonistic, additive interaction) generally, without taking into the consideration the change of this interaction during the cultivation. The purpose of the research was the development of a new approach of the quantitative determination of the parameter of the interaction of microorganisms of one species with another species expressed as a continuous function determined throughout the all fermentation period. In the process of the work a two-component consortium of lactic acid microorganisms Lactobacillus brevis and Lactobacillus plantarum was selected, cultivated in two different model media as part of a study of directed fermentation of vegetable products. The work the cultivation of monocultures (included in the studied consortium) was also carried out. The performed work the original approach was developed, the approach is based on a comparison of the growth rate of the microorganisms biomass of the consortium with the calculated additive dynamics. This approach is convenient to use to identify a complex regularity of changes of the types of interaction of microorganisms in the consortium in the form of a continuous function determined during the entire cultivation period. From the point of view of the synergies of the species of microorganisms entering the studied consortium in the active phase of fermentation, the "Slava" variety turned to be more profitable than the "Parus".
Show more [+] Less [-]COMPARATIVE ASSESSMENT OF POTATO SPECIES IN THE PERM REGION CONDITIONS
2018
N. V. Chukhlantsev | D. S. Fomin
Potatoes in all categories of farms in the Permskii Krai occupy 41.3 thousand hectares, in agricultural enterprises – 4.5 thousand hectares, with an average yield for 2014-2016 of 13.9 t / ha. The Permskii Krai there are all the prerequisites for obtaining high yields of potatoes. One of them is potato seed production. Variety, one of the important tasks of seed production, without it, with the ever-increasing need for new universal varieties that combine preservation of the original qualities for as long as possible, i.e. high stable productivity, early yield accumulation, good culinary and technical qualities (starchiness, good keeping quality, etc.) with resistance to the most harmful diseases, pests and adverse environmental conditions cannot be dispensed with. The purpose of research: the identification of promising varieties of potatoes, highly productive, in the Perm region. Investigations were carried out in 2011-2016 at the central experimental field of the Perm Agricultural Research Institute Branch of Perm Federal Research Center of Russian Academy of Science. In the collection nursery, 33 varieties of three groups of precocity were tested. A comparative assessment of potato varieties by yield and economic characteristics is presented. Promising potato varieties for cultivation under the conditions of the Perm Territory were identified in the group of early maturing: Albatross, Nandina, Latona, medium-early varieties: Irbit, 428- 05, Bravo, Miner, Amur. The varieties Crisper, Lukoshko, Bafana were the best in selected indicators in the mid-season group. Comparative assessment of potato varieties according to yield and economic characteristics is presented; studies were carried out at the Perm Agricultural Research Institute Branch of Perm Federal Research Center of Russian Academy of Science in the soil and climatic conditions of 2011-2016. Promising potato varieties for cultivation under the conditions of the Permskii Krai were identified in the group of early maturing: Albatross, Nandina, Latona, medium-early varieties: Irbit, 428-05, Bravo, Miner, Amur. The varieties Crisper, Lukoshko, Bafana were the best in selected indicators in the mid-season group.
Show more [+] Less [-]DISEASES OF BROAD BEANS CAUSED BY MICROSCOPIC FUNGI
2018
Yu. N. Kurkina
The unique biochemical composition of broad bean seeds (Vicia faba L. var. major Harz) makes them a promising vegetable crop. The quantity and quality of the yield of beans depends to a large extent on the mycoses caused by microscopic fungi. The aim of the work was to study the diseases of broad beans caused by microscopic fungi in the soil and climatic conditions of the South Central zone of the region. At the Botanical garden BelSU (Belgorod) on a natural infectious background were grown and studied of different broad beans varieties Belarusian, Velena, Russian black, Aquadulche. In some years, the prevalence of Alternaria leaf spot was 8-57%, but the lack of yield did not exceed 12%. Fusarium wilt was spread to 90%, with a disease development index of up to 82% and a loss of yield of 16-64%. In cases of mixed infection (fusariosis + alternariosis, fusariosis + chocolate spot) on plants in the budding-flowering phase (before and after the phase of fruit formation), the destruction of affected plants was noted. The spread of mixed infection was prevented by dry weather during the development of plant generative organs. The development of bean leaf and pod spot, which reduced the productivity by no more than 15%, contributed to the dry period of bean growth. Chocolate spot (not marked on varieties Russian black and Velena) and blackish spotting were noted not on all grades and not annually, reducing the yield of beans by 2-10%. In general, over the years of research, the lowest losses of yield from mycoses are noted for the Belarusian.
Show more [+] Less [-]THE MAIN ASPECTS OF REGULATING THE IMPORT OF VEGETABLES TO RUSSIA
2018
I. V. Pogodina | R. S. Streltsov
The article describes the main aspects of regulating the import of vegetables to Russia, the statistical data allow to make conclusion about the current state of the import substitution of vegetable products, the examples allow to assess the economic and political situation in Russia and in countries directly related to trade and other relations with Russia. Also partially affected the overall situation of export of vegetable products, perspectives and possible problems in this direction. Presents issues to consider allow closer approach to the solution of the main strategic problems of the country – the full import substitution of Russian product. The problem in this area is impossible to solve. Russian manufacturers that make quality products that do not have sufficient capacity to provide the whole of Russia. In addition, the Russian agricultural sector has a serious dependence on foreign high-tech solutions. Thus, measures of tariff and non-tariff regulation is required to affect the import of vegetables.
Show more [+] Less [-]NASLEDNIK – A NEW VARIETY OF CUCUMBER OF THE FAR EASTERN BREEDING
2018
N. V. Kulyakina | T. K. Yurechko | G. A. Kuzmitskaya
Breeding work on cucumber culture is conducted in many scientific research institutions and selection and seed-growing firms. The priority direction in the breeding of cucumber in the Far Eastern Research Institute of Agriculture is the creation of bee-dusted varieties for open ground, adapted to local conditions with a sharply variable hydrothermal regime and a high natural infectious background, where the most dangerous of cucumber diseases are downy mildew (peronosporosis) and angular spotting of the leafs (bacteriosis). The breeding and introduction of disease-resistant varieties and hybrids in to practice is the most effective and cheapest method of combating plant diseases, as it is the only way you can get guaranteed yields. A new variety of cucumber Naslednik was obtained as a result of testing promising cucumber varieties at the Far Eastern Research Institute of Agriculture and was handed over in 2016 in the State Variety Test. The variety is medium-ripening, bee-dusted. The fruiting occurs 44-47 days after the emergence of mass shoots. The green fruits are elongate-ovoid form 10-12 cm long, 3.5-4.0 cm in diameter and with a fruit weight of 100-120 g. The average total yield of the fruit is 38.9 t/ha, the marketable – to 31.6 t/ha, respectively to the standard (variety Mig) obtained 35.1 t / ha and 27.4 t/ha. The most valuable qualities of the new variety are resistance to peronosporosis and bacteriosis, a high yield of marketable fruits (80-82%), slowly turning brow fruits.
Show more [+] Less [-]PROMISING WATERMELON VARIETY ‘METEOR’
2018
S. V. Malueva | E. A. Varivoda | I. N. Bocherova
Watermelon is one of the most common crops of medium melons. Biological peculiarities of cultivation of watermelon allow using global warming as a factor expanding the area for cultivation. Breeding work is carried out to develop new varieties and hybrids of watermelon suitable for cultivation in more Northern areas of the Russian Federation with a shorter vegetation period In Bykovskaya Melons and Gourds Experimental Breeding Station. To accomplish this goal the creation of new varieties of watermelon, early maturity with excellent taste, friendly fruit ripening, drought-resistant, suitable for intensive cultivation technology is carried out. Currently, the new early maturing variety of watermelon ‘Meteor’ with a growing period for 65-70 days and dry matter content to 12.0% has been transferred to the State Variety Trial. According to research results, comparative characteristics between new promising variety ‘Meteor’ and control variety ‘Zenith’ were given. The ‘Meteor exceeded the standard variety in yield ability by 19.0 сwt/ha. According to pathogen tests for complex resistance the ‘Meteor’ exceeded the control by 6.4 % for Fusarium, and scored at 0.8 against Anthracnose attack.
Show more [+] Less [-]EFFICIENCY OF NATURAL GROWTH REGULATORS IN CARROT PRODUCTION
2018
N. E. Maschenko | A. D. Borovskaya | A. V. Gumaniuk | I. T. Balashova | E. G. Cozar
Utilization of natural biostimulators- secondary metabolits of plants in carrot production was studied. Purified fractions with glycoside content are characterized with extraction and adsorption chromatography from the next plants: linarosides – from Linaria vulgaris Mill. L., melampyrosides - from Melampyrum nemorosum L., genistifoliosides - from Linaria genistifolia L., verbascosides - from Verbascum phlomoides L., melongosides - from Solanum melongena L. and tomatoside – from Solanum lycopersicum L. The former substance was registered under market mark “ecostim”. Prospective preparations for the improvement plant resistance to abiotic and biotic stresses have been identified in laboratory tests. Treatment of carrot seeds with water solutions of such substances accelerates plant growth, increases yield and improves quality of storage roots. We determined regimes of seed treatment (substance concentration, time of exposition) and gave recommends by using of natural regulators in the technologic scheme of carrot cultivation. Using secondary metabolites of higher plants stimulates the energy of germination (on 1,5-6,7%) and increases the field germination of carrot seeds (on 23-40%). It accelerates leaves growth and increases the mass of storage roots, improves biochemical characteristics of storage roots (accumulation the β- carotene and decreasing of nitrates content). Yield of storage roots increases on 4,2-8,5 t/ha, and yield of market quality roots increases on 4-6 t/ha.
Show more [+] Less [-]PROMISING ACCESSIONS OF CHAENOMELES AND THEIR USE IN THE FUNCTIONAL FOOD
2018
V. N. Sorokopudov | O. A. Sorokopudova | A. G. Kuklina | A. В. Artjukhova | N. I. Myachikova
A complex analysis is crucial for obtaining new resistant varieties and developing recommendations for the use of the fruit of Chaenomeles. The task of this study was to assess the productivity and quality of the fruit of the selected forms of Chaenomeles in Central Russia, with the determination of the possibility of a no-waste technology for fruit processing, and the appropriateness of using functional food in the composition of products. The studies were conducted in 2012-2016. In the Botanical Gardens of the National Research University "BelGU" (Belgorod), in the FGBNU VSTISP and GBS N.V. Tsitsina. As materials for the study, 6 selective forms of Chaenomeles, obtained from free pollination of the ‘Calif’ variety, used as a control, were used. The study was carried out according to the generally accepted methodology of varietal studies, along with using the authors' methodical development. A sufficiently high nutritional and biological value of the chelating of the Chaenomeles fruit has been observed. At the same time, the minerals, carbohydrates and vitamins from entire fruits exceed the content of ones squeezed from the pulp. The obtained results of the studies allow us to conclude that it is advisable to organize a non-waste technology for the processing of fruit of Chaenomeles, which can serve as one of the components for the enrichment of food products. Thus, a comprehensive assessment of the biological properties and productivity of breeding forms of Chaenomeles has been made showing that they exceeded the parent variety in stability are regarded as promising intense-vitamin fruit culture, that can be used for various processing methods, as part of functional and therapeutic product-prophylactic nutrition, especially in obtaining natural low-calorie foods.
Show more [+] Less [-]ANTHECOLOGY OF SOME SPECIES OF THE GENUS ALLIUM L. UNDER MOSCOW PROVINCE CONDITIONS
2018
F. V. Golubev
Different perennial species of Allium L. have been studied under Moscow Province conditions as new vegetables and melliferous plants. The results of long-term studies of anthecology of seven species of Allium L.: A. nutans L., A. flavescens Bess., A. odorum L., A. montanum F.W. Schmidt, A. fistulosum L., A. angulosum L., A. schoenoprasum L. are presented in the paper. It is shown that the objects of study differ in terms of the beginning of flowering and length of the growing season. The early-flowering (A. fistulosum, A. montanum, A. schoenoprasum), and late-flowering species (A. angulosum, A. nutans, A. odorum, A. flavescens) were identified. The species features in the daily dynamics of flowering, the timing of development of pistils and the duration of susceptibility of their stigmas are revealed. The significant influence of weather conditions on these processes is established. By nature of blooming flowers within a single inflorescence type are revealed apical (A. schoenoprasum, A. fistulosum) and basal (A. odorum, A. nutans, A. montanum, A. flavescens) types of inflorescence. Besides, groups of species differing with the dynamics of disclosure of flowers in inflorescences are noted. It was found that the daily course of opening flowers within the species, as a whole, is constant, but depends on weather conditions. It is shown that the maximum number of flowers opening per day in all species falls on the middle of the flowering period. The species composition of pollinators and entomophages for the studied species of Allium L. was determined. The taxonomic diversity of the composition of insects visiting the inflorescences of onions is shown. The role of separate insects in the pollination process is established. The main pollinators of early-flowering and late-flowering species of onions are determined. It is stated that the most important as pollinators are species related to the orders of Hymenoptera (bumblebees, bees) and Diptera (flower flies, tachina flies), the activity of which largely depends on weather conditions and time of day.
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