Melon (Cucumis melo L.) is the most spread melon crop in Uzbekistan. More than 130 local varieties of melon are cultivated in various regions of the republic. Every year melons and gourds are cultivated on the area of 150 thousand hectares. The most harmful diseases of the melon are powdery mildew and Fusarium wilt. All local varieties of melon are susceptible to these diseases. Annually from these diseases, the yield of melon is reduced by 20-25%. The most effective way to combat these diseases is to develop resistant varieties. The Research Institute of Vegetable, Melon Crops and Potato has carried out long-term selection work to create melon varieties resistant to powdery mildew and Fusarium wilt. Breeding local varieties of melon with economically valuable traits and high test quality with genes for resistance to these diseases have been created and included in the State Register of Uzbekistan.

Introduction: Anthocyanins, the polyphenolic plant pigments, have high antioxidant activity (AOA), reduce the risks of many pathological conditions in the human body. However, the wide medical and preventive use of anthocyanins is limited by their degradation during processing of plant raw materials. The objective of the work was to study the anthocyanins’ stability by spectrophotometric method during heat treatment and storage of vegetable and berry plants.Study objects and methods: Purple potatoes, eggplants, red cabbage, purple carrots, blue onions, red raspberries and blue honeysuckle were used in the study. Anthocyanins’ stability was determined by numerical values of extracts’ absorption spectra from the studied plants.Results and discussion: High stability indices of 0.623–0.986 were obtained for the anthocyanins of purple carrots, blue onion bulbs or red cabbage leaves whose main component is the antioxidant cyanidin with a relative AOA equal to 3.49. Low stability indices of 0.229-0.23 were obtained for anthocyanins of red raspberry berries and purple potato tubers containing pelargonidin or malvidin with 2.49–3.36 times lower relative AOA than for cyanidin. A regular correlation between stability and AOA of anthocyanins with a rank correlation coefficient of 0.91 (p<0.05) was established.It was established that during three months of storage in a domestic refrigerator, the content of anthocyanins in purple carrots and purple potatoes decreased by 10–15% of the initial one, and this decrease was 2-3 times greater when other studied plants were stored for 1.5–2 months.Conclusion: It is advisable to use the developed spectrophotometric methods in the express-analysis at selection of perspective plants for industrial cultivation as raw material for anthocyanin-containing herbal formulation.

Timeliness. Currently, the Russian Federation has a high demand in healthy planting material of garden strawberries. The benefits of using fertilizers when growing strawberry seedlings have been proven by numerous studies. However, a comparative study of the effect of new fertilizers and methods of their application is very limited. In this regard, studies aimed at identifying the effectiveness of the use of prolonged-acting fertilizers in the production of garden strawberry planting material in protected soil conditions are relevant.Methods. Adapted to non-sterile conditions, ex vitro healthy garden strawberry plants were planted in a peat substrate of Veltorf LLC (recipe No. 5c/2) with the addition of Pg mix starter fertilizer with the structure NPK 14-16-18 + microelements (1.5 g/l of substrate). At the same time, prolonged-acting fertilizers were introduced into the substrate in the recommended concentrations: FertiPro Gran 1M (1.25 g/l of substrate), Ruscote 5-6M (3.0 g/l of substrate), Basacote Plus 6M (4.0 g/l of substrate), control peat substrate without fertilizers, PG mix standard.Results. It has been established that for the Karmen crop with an average runner-forming ability, the effectiveness of applying FertiPro Gran 1M fertilizer to the substrate was revealed, with the use of which, on the 180th day of cultivation, the number of runners and rosettes increases by 3.5–3.8 times compared to the control. At the same time, the yield of marketable seedlings is 95% compared to 70% in the control. For the Jūnija smaids crop with a high vine-forming ability, the effectiveness of all experimental variants with the application of FertiPro Gran 1M, Ruscote and Basacote Plus fertilizers to the substrate was revealed, with the use of which, on the 180th day of cultivation, the number of runners and rosettes increases by 1,9-2,5 times compared to the control. At the same time, the yield of marketable seedlings is 94-96% compared to 65% in the control.Conclusion. The introduction of long–acting fertilizers FertiPro Gran, Ruscote and Basacote Plus into the peat substrate with the addition of Pg mix starter fertilizer allowed a 2.3 – 3.8-fold increase in the number of runners obtained and 1.9-3.5-fold increase in the number of sockets obtained compared to the control without fertilizers.

Coriander is the leading essential oil crop and occupies vast areas, both in our country and abroad, and is be used to produce linalool, with subsequent processing into other aromatic substances. Increasing its yield and quality of raw materials with the help of modern preparations is an urgent problem of essential oil production.Purpose of the work: increasing the productivity of coriander with combined use of foliar treatments with glycine and auxin-containing preparations for directed control production process in coriander seed.Material and methods. As objects for studying the effect of the drug, varieties of coriander sowing Yantar and Avangard were chosen. Plants were sown in the first or third decade of April, depending on the conditions of the year, using a SZT-3.6 seeder with row spacing of 15 cm. The seeding rate was 25 kg/ha, the seeding depth was 2 cm. concentration 10 mg/l. Drug concentrations: IAA-glycol phosphate – 25, 50 and 100 mg/l, DvaU - 2 ml/l. Treatment with a solution of IUKGF and DvaU was carried out in the phase of budding-beginning of flowering. The cutting was carried out during the period of browning of seeds on the central umbrella. The content of essential oil was determined according to SP XIV method 1.Results. As a result of the research, a positive effect of foliar treatments with glycine was revealed, both on the yield and on the content of essential oil in the raw material of coriander seed varieties Yantar and Avangard. Based on the results obtained, the effective concentration of the drug is determined not only by the characteristics of the variety, but also by weather conditions, when, depending on the conditions during the processing period and the previous harvest, different aspects of the drug action appear. The maximum increase in the collection of essential oil of coriander variety Yantar (11.1 kg/ha) was noted during the combined treatment of plants with glycine at a rate of 10 mg/l in the leaf rosette phase and IAA-GF at a rate of 50 mg/l in the budding phase; varieties Avangard (8.6 kg/ha) - with combined treatment of plants with glycine at a rate of 10 mg/l in the leaf rosette phase and DvaU at a rate of 2 ml/l in the budding phase.

Production of functional food with high levels of selenium and other antioxidants is very valuable for human protection against different forms of oxidant stress. Among leafy vegetables parsley demonstrate the highest levels of antioxidants. Biochemical analysis and fluorimetric determination of selenium revealed that foliar biofortification of 4 parsley (Petroselinum crispum) cultivars and Mitsuba (Cryptotaenia japonica) with sodium selenate (25 mg L-1) resulted in the highest biofortification level in curly parsley cultivar Krasotka (102.9) which showed the highest leaf surface area, antioxidant activity (65 mg GAE g-1 d.w.) and flavonoids content (25.9 mg quercetin equivalent g-1 d.w.), and the increase by 1.4 times in carotene content and 1,5 times in total chlorophyll content. ICP-MS method of mineral composition evaluation recorded extremely high levels of B and Si in Mustuba, which increased due to Se supplementation by 1.23 and 1.46 times respectively. In a two-year experiment with control and Se-fortified, leafy parsley, cultivar Moskvichka reached high values of seed yield and viability, and seed Se content (6170 µg kg-1 d.w.). The results of the present investigation demonstrate high prospects of parsley and Mitsuba selenium biofortification for production of functional food with elevated levels of microelement and high antioxidant activity.

Relevance. Mash (Vigna radiate (L.) R.Wilczek) is a very ancient Asian legume plant, which, thanks to a number of advantages, may become an important vegetable crop in Russia. Young fruits and seeds of Vigna radiate are rich in protein, vitamins A, B, C, PP, iron, folic acid, and taste like green peas. A lot of essential amino acids and a low glycemic index make it possible to use even mature mash seeds for the preparation of dietary food. To preserve the harvest, it is important to know the diseases of the crop.Methods. For a number of years, a sample of the radiant vigna VR-2014 (Vietnam) has been studied on a natural infectious background in the soil and climatic conditions of the Belgorod region (Russia). Field tests and mycological studies made it possible to determine the species composition of phytopathogenic microscopic fungi, describe the symptoms and compare the main elements of accounting for mycoses.Results. The length of the growing season of the radiant vigna did not exceed 83 days, the appearance of seedlings was noted on 10-12 days from sowing, flowers – on 68-73 days. The height of the plants did not exceed 40 cm. Ascochytosis (the causative agent of Ascochyta boltschauseri) led to losses of 82% of seed productivity, with an average prevalence of 40% and the development of the disease of 2.5 points. Ascochitosis reduced the values of all elements of seed productivity: the length and number of fruits, the mass of 1000 seeds, but most of all (by 5 times) the weight of fruits and seeds from the plant decreased. Peronosporosis (the causative agent of Peronospora fabae) led to losses of seed productivity by 16%, root rot (the causative agent of Bipolaris australensis) and fomosis (the causative agent of Phoma diversispora) – by 17% and 11%, respectively. Spotting was the leading symptom of mycoses of Vigna radiate, and the most harmful phytopathogenic fungi were representatives of the ground-air group of pathogens.

In agriculture, the use of microbiological fertilizers is becoming more and more widespread. The presence of microorganisms in them allows improving soil fertility, increasing the productivity of crops, while ensuring the cultivation of environmentally friendly products. There is a wide variety of microbiological fertilizers, so determining the type of preparation for garden strawberries when grown in the conditions of the Udmurt Republic is relevant. The purpose of the research: a comparative assessment of the effect of various types of microbiological fertilizers on the productivity of garden strawberries.Materials and Methods. Studies on the effect of microbiological fertilizers on the yield and its structure of garden strawberries were carried out on the territory of Vostochny LLC, Zavyalovsky District, Udmurt Republic. In 2016, 2018 studies were carried out on the feeding of garden strawberries of the Darenka vari- ety of the first and third years of fruiting with microbiological fertilizers on soddy-medium-podzolic medium-loamy soil. The scheme of the experiment included the following options: Water (control), Baikal EM 1, Emix, Humat EM. The total area of the plot is 2,9 m2. The accounting area of the plot is 1,8 m2. Placement of variants by a systematic method, in six-fold repetition.Results. Harvesting was carried out in five terms. Studies have proven the positive effect of the studied microbiological fertilizers on plant productivity. The highest yield of berries was 833,6 g/m2, which was obtained using Baikal EM 1. The largest berry was obtained in 2016, also when treated with Baikal EM 1, and amounted to 145 g. lo to increase the number of berries from the bush. So, in 2016 their number was 45,2; in 2018 – 42,0 pieces.

Scientific relevance. Plant viruses cause a significant economic loss to potato production, especially if plants are infected at early growth stages and infections are mixed. Viral diseases reduce both yield and quality of harvested crops. Detection and identification of plant viruses are key important to prevent their spreading and to achieve potential yield predetermined by characteristics of varieties.Research methods. Seven potato varieties, bred in Russia and overseas, were used in the field experiment: Smak, Avgustin, Yantar, Laperla, Labella, Red Lady, Sante, Belmonda. Viral infection rate was measured by the percent of plants with symptoms to the total number of plants. In addition to infection frequency, a disease rate was described after visual estimation. Total RNA was isolated from the collected leaves according to Bekesiova I. et al. 1999 [13]. Qualitative and quantitative estimation of plant viruses in the samples were conducted by single-step real-time RT-PCR with fluorescent detection with the Applied Biosystems QuantStudio 5 and commercial kits “Potato Virus X, Y, M, L, S, A, PSTVd-RT” (Syntol Company) according to the official protocol of the kits.Results. As a result of our research, symptoms of mixed viral infection were described for potato varieties depending on concentrations and proportions of these viruses in a plant. Mixed viral infection in the potato field in Primorsky Krai comprised PVY, PVX, PVA, PVS, PVM, also PLRV and PSTVd.

The aim of the study was to determine the growth, development and yield of sweet corn varieties and hybrids as vegetables during the soil-climatic conditions of Karakalpakstan.Methods. The 8 cultivars and 8 hybrids studied were evaluated for growth, development and yield in Karakalpakstan in 2017-2019. Selection work was carried out on varieties and hybrids. Varieties and hybrids with a positive result based on the selection results were recommended for use in subsequent experiments.Results. After sowing the seeds of the studied 8 varieties and 8 hybrids of vegetable (sweet) corn, the duration of the germination period was 7-9 days. Relatively early germination between cultivars and hybrids Zamin, Can. Pedro 2 Inta, Berys varieties, relatively late germination was recorded in 9 days in SPV 1022, Leonard's Early, Honey Bontam F1, Spirit F1, Megaton F1, Baron F1, Soyan F1, Hybrid F1. It has been established that the period from germination to the stage of leaf formation (7 leaves) is 13-16 days, the period from the stage of leaf formation (7 leaves)to the panicle formation is 23-33 days, the period from the panicle formation to flowering is 4-6 days, the period from flowering to the cob formation is 9-15 days. In the studied varieties and hybrids, the period from the cob formation to milky ripeness was 13-18 days, from milky ripeness to waxy ripeness lasted up to 4-8 days.The study of the duration of the development phases of varieties and hybrids showed that the growth period from germination to milky-wax ripeness is 75-85 days. Early yield was observed in hybrids Baron F1, Sweet star F1, Spirit F1, Soyan F1, vegetation period 73-77 days, relatively early yield was observed in varieties and hybrids Zamin, Sentinel F1, SPV 1022, Osnova 209, Hybrid F1, Can Pedro 2 Inta, Clx3349ys clause, growing season 79-81 days.The selection of varieties and hybrids most appropriate to the soil and climatic conditions of Karakalpakstan was carried out and recommended for cultivation by farmers and dekhkan farms.

Relevance. As a result of long-term research, the staff of the Department of Agronomy, Plant Breeding and Seed Production of Omsk State Agrarian University created a new variety of green beans called Marusya, which was included in the State Register of Breeding Achievements in 2015. The competitiveness of the variety is ensured by stable yields, high palatability, and the ability of green beans to maintain their economic viability over the long period. The variety is resistant to drought and low temperatures.Material and methods. The research on the variety of the green beans was conducted following the methods of competitive variety trial from 2016 to 2020. The trials were conducted on the breeding crop rotation fields of the Educational and Experimental Farm of Omsk State Agrarian University, which is located in the southern forest-steppe of the Omsk Region (city of Omsk). The following zoned varieties were used as control samples: Zolushka (Federal Scientific Center of Vegetable Farming, Moscow) and the imported Polka variety (Poland).Results. The developed agroecological passport the Marusya variety, the use of which will allow to take into account the positive effects of the genotype-environment-variety interaction to the maximum extent. When selecting bean varieties suitable for cultivation for vegetable purposes in the conditions of the southern forest-steppe of Western Siberia in industrial production and the private sector, the Marusya variety makes it possible to obtain high-quality products and expand the range of legumes in the region.