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FUNGAL DISEASES OF ASPARAGUS VIGNA IN THE SOUTH OF WESTERN SIBERIA
2019
Yu. V. Fotev | O. A. Kazakova
Increasing popularity of asparagus bean (Vigna unguiculata (L.) Walp.) − a new for Russia vegetable crop requires comprehensive study including resistance to biotic environmental factors. Composition of pathogens of germinating seeds, plants (roots, leaves, stems, fruits) of large collection of asparagus bean from the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) and the Bioresource Scientific Collection of the CSBG SB RAS UNU No. USU 440534 have been investigated. Plants of 87 samples of asparagus bean were grown in the unheated plastic film greenhouse on the south of Western Siberia (54 ° N lat. 83 ° E) and examined for infestations of diseases in the Central Siberian Botanical Garden and Novosibirsk State Agrarian University (Novosibirsk). The infection of seeds with pathogenic micromycetes was determined according to GOST 12044-93 (similar to common beans) using the methods of mycological analysis and the Petri-dish moist chambers, also on Chapek media and KDA media. The main pathogenic micromycetes are: on germinating seeds − species of the genera Fusarium, Aspergillus, Penicillium, Alternaria, Mucor, Botrytis cinerea, Sclerotinia sclerotiorum; on seedlings − Fusarium solani; during fruiting on fruits and stems − B. cinerea and S. sclerotiorum; on the root collar − S. sclerotiorum. The dominant forms of white mold were stem, root collar, fruits. Five samples that showed a high level of resistance to B.cinerea and S.sclerotiorum have been selected. System for asparagus bean disease surveillance which should be built in the calendar-phenological sequence, has been recommended.
Show more [+] Less [-]The results of the comparative evaluation of samples of oat (<i>A. sativa</i> L., <i>A. byzantine</i> C. Koch) for resistance to lodging, yield and adaptability in central Non-chernozem zone of the Russian Federation
2019
Julia I. Vargach
Relevance. Each sample is characterized by a number of biological features, due to the recombination of genetic material in the process of its selection. These features are manifested in the reaction of plants to soil-climatic and technological conditions, expressed in the change of the elements of the yield structure and indicators of the level of adaptability of plants to growing conditions, as well as resistance to lodging. New samples have little-known properties that need to be studied and compared in specific soil and climatic conditions, to assess them in order to decide on the possibility of their zoning or culling. The main indicators characterizing the level of agricultural production are productivity, resistance to lodging, since the shortage of grain as a result of lodging of cereals can reach 15-40%. The introduction of new high-yielding varieties on the basis of their testing and the use of the most effective technological methods of cultivation on crops will allow to receive a significant gross harvest.Materials and methods. The study was carried out in 2016-2018 to assess models of cultural hexaploid species of oats hulled and naked forms of resistance to lodging, yield and adaptability in the temperate continental climate of Central non-Chernozem zone. Resistance to lodging, yield variability, indices of environmental conditions and adaptability were determined. The main method of evaluation of the field resistance to lodging – visual (in points) that allows you to rank the varieties based on this characteristic. We were calculated potential productivity and adaptability of varieties by method L. A. Zhivotkov et al. [1], an indicator of the stability level of the sample yield (Puss) by the method of E. D. Nettevich et al. [2].Results. Many varieties showed good plasticity and high productivity potential. The best in resistance to lodging, yield and adaptability among the hulled forms were varieties Sig, Mirt, Poseidon, Belinda, Rajtar, and the naked forms – Becas, Vyatsky, Korolek.
Show more [+] Less [-]Antioxidant activity of potatoes (Solanum tuberosum L.) and anthocyanin content, its biosynthesis and physiological role
2019
O. B. Polivanova | E. M. Gins
Relevance. Potatoes with red and purple flesh are highly valued dietary products and sources of polyphenolic compounds with antioxidant activity, especially anthocyanins. It has been found that the anthocyanin content in potatoes correlates with the total soluble phenolic content and antioxidant activity. These measures are significantly higher for coloured potatoes. The total phenolic content in particular the total anthocyanin content increases during the cold storage of coloured potatoes. Pigmented potatoes are also more preferable for food and industrial processing because of retaining of antioxidant properties and improving of final products quality. Сoloured potatoes selection and biotechnological approaches of regulation of anthocyanins accumulation are based on the understanding of the molecular genetic processes of anthocyanins biosynthesis. The anthocyanin biosynthesis is a part of the phenylpropanoid metabolic pathway. It is controlled by the MBW complex. The MBW complex includes the transcription factors MYB, bHLH, and WD40. A number of the MYB complex genes have been identified in potatoes. The key role in potatoes pigmentation variability is currently assigned to the StAN1 gene. According to available data anthocyanins play a protective role in response to various types of stress in potatoes.
Show more [+] Less [-]INFLUENCE OF GENUS BACTERIA <i>BACILLUS</i> ON QUALITY OF <i>Lactuca sativa L.</i>
2019
O. V. Doroshchuk | J. N. Kalatskaja | N. A. Laman | V. V. Minkova | M. N. Mandrik-Litvinkovich
Primal problem of vegetable growing is constant supply of the population with all types of vegetables, including green cultures. Green cultures are vegetables that have high nutritional value and precocity. However they often are infected by phytopathogenic microorganisms already at initial stages of ontogenesis at cultivation in closed soil conditions. It leads to emergence of disjointed shoots, deterioration of growth and development of plants and loss of quality. Now in the Republic of Belarus a number of biological substances on the basis of bacteria Bacillus was developed. They are used against diseases of plants of mushroom and bacterial etiology. However there is not information about influence of bacteria on quality of products of green cultures. The aim of the work was studying of influence of two strains of bacteria Bacillus that were introduced in peat substrate on efficiency and quality of lettuce. Two strains of bacteria Bacillus were used in the work. They were selected from the soil. The strains are Bacillus subtilis M9/6 and Bacillus amyloliquefaciens 23TM that have high antagonistic activity to a wide range of phytopathogens. Cultivation of plants carried out in containers of 250 ml under light installations with illuminating intensity 13-15 thousand luxury and lasting irradiating of 14 hours before technical ripeness of lettuce. It was established that the application of strain Bacillus subtilis M9/6 (in concentration 106 cells/ml, 10 ml/l of substrate) and the strain Bacillus amyloliquefaciens 23TM (5 ml/l of substrate) in substrate before sowing increased nutrition value of lettuce. Dry matter content, water-soluble carbohydrates (mono - and disaccharides) content and vitamin C content increased. The bacterial strain B. amyloliquefaciens 23TM also promoted accumulation of vitamin B2. The content of nitrates in lettuce leaves decreased on 50,3% and 39,1%, respectively. It was shown that the application of bacteria in substrate before sowing of crop has a greater influence on quality of lettuce, than watering of shoots.
Show more [+] Less [-]SELECTION OF PARENTAL COMPONENTS OF SWEET PEPPER HYBRIDS FOR STEPPE AND DRY STEPPE
2019
E. A. Kozlovskaya | O. N. Pyshnaya | M. I. Mamedov | E. A. Dzhos | A. A. Matyukina
The main direction in the selection of sweet peppers is the creation of hybrids with a high heterosis effect. The question of components choice for the greatest effect in crossings is studied by breeders and geneticists of many countries for a long time. However, until now it has not been possible to create a theory that allows breeders conducting practical work to consciously and reliably choose pairs that would provide maxi-mum and stable yield and product quality, depending on the purpose of the variety. In the work various principles of the selection of pairs were used: ecological-geographical, according to the components of attributes and selection of the maternal form. Using these methods, more than 300 combinations of crosses were obtained. It is shown that the combination of two ecologically different parental components of different origin - the local sample and the introduced one - leads to an increase in the heterosis effect. The best hybrid combinations with a complex of economically valuable traits are obtained by crossing local varieties with selected breeding lines. When selecting pairs according to the components of the signs, where local or introduced samples with a different set of features were taken as parental forms, productivity was at the level of the standard. In most cases, when used as the maternal form of the local variety, the productivity of the hybrids was significantly higher compared to the reverse crossing. As a result of our studies, it is once again confirmed and proved that the more differences in the places of cultivation and reproduction of parental components, the higher the heterosis effect and the effect of the maternal component is much higher.
Show more [+] Less [-]STRUCTURE OF SOIL COMPLEXES OF MICROSCOPIC FUNGI UNDER DIFFERENT VARIETIES OF BROAD BEANS
2019
Yu. N. Kurkina
Considering of broad beans as an integral component of biological crop rotations, the presence of phytopathogenic, toxigenic, opportunistic, and allergenic microscopic fungi in soils under their different varieties should be examined. At the Botanical garden BelSU (Belgorod) on a natural infectious background were grown and studied of 16 different broad beans varieties. Were taking into account the indicators of frequency of occurrence and abundance of species, they determined the structures of soil mycocomplexes under different varieties. Analysis of the data showed that most micromycetes in the rhizosphere under bean varieties belonged to the Ascomycota division, which is part of it as 4th classes of Sordariomycetes, Dothideomycetes, Eurotiomycetes and Saccharomycetes. The species diversity of the soil mycobiota of steaming soil was characterized by 26 species, and under the studied bean varieties 57 species of fungi were identified. In the mycocomplex under varieties, species of different ranks of frequency of occurrence developed that were not noted in the paring soil: Alternaria tenuissima and Stemphyllium solani were rarely and accidentally found in the control soil, but they were not found in under the beans during budding – flowering. This can be explained by the fact that a different composition of exudates in the root zone of the soil under the plants contributes to the development of different types of microorganisms. In the complex of micromycetes under the beans, the share of toxigenic species increased by 2.5-29%, opportunistic species - by 1.3-31%, allergens - by 2-24% compared with the soaring soil. In the soil, an abundance of opportunistic and allergenic species capable of inducing mycoses and allergies in humans can be reduced by the broad bean varieties Aquadul, White large-fruited, Russian Black, Velena, Summer Resident, Leader, Optics, Pink Flamingo, Yankel Byala. After the cultivation of the beans the phytopathogenic species Ascochyta fabae, Clasdosporium herbarum, Fusarium graminearum, F. oxysporum var. orthoceras, F. oxysporum, F. solani and Ulocladium botrytis, remain in the soil and this fact must be considered when selecting the next crop in crop rotation.
Show more [+] Less [-]LARGE-FRUITED, DELICACY AND HIGHLY PALATABLE TOMATO VARIETIES
2019
I. Yu. Kondratyeva | M. R. Engalychev
The conflict has arisen between the production of “technical”, low quality tomato varieties in the interests of farmers and processers and the need of population accustomed to consumption of salad tomatoes in high quality, useful products. As far as now this conflict has not been settled. Tomatoes are among the most important year-round sources of vitamins С, В, В2, РР, А, Н, В9, pectines, valuable minerals and nutrients and also other compounds, especially the antioxidants lycopene and carotene, which determine healthy diet of humans and protect from many diseases. It is necessary to create the tomato varieties for each soil-climatic zone, which are adapted to specific environmental conditions of cultivation, thus maximizing the genetic and climatic potential of the crop. In non-chernozem zone of Russia, which is characterized by perhumidity but rather short vegetation period and insufficient sum of temperatures for such warm-weather crop as tomato, the plant breeders of Federal State-Financed research Institution, Federal Research Center of Vegetable Production “VNIISSOK” create primarily short-season and mi-season varieties, which are tolerant to the low temperatures, short-growth (tree-type and determinant bushes) with closely placed clusters, even ripening and resistant to shot holes.
Show more [+] Less [-]Methodological aspects related to biological assessment of insectoacaricides on flower cultures of protected ground
2019
T. V. Dolzhenko | V. I. Makarenko | L. A. Burkova
Relevance. Analysis of the present range of pesticides that are used in flower orangeries showed critical shortage of allowed pesticides. Now there are no methods for registration testing of insecticides designed to protect flowers in greenhouses. Thus, the purpose of our research is to develop methodological approaches to assessment of abundance of major pests for flower cultures of protected ground and determination of biological effectiveness of new insectoacaricides.Methods. The following phytophages represent flower pests that are predominant in the protected ground: aphids: green peach – Myzus persicae Sulz., melon and cotton – Aphis gossypii Glov., greenhouse-potato – Aulacorthum solani Kalt., potato – Macrosiphum euphorbiae Thom., green rose – Macrosiphum rosae Linn.; whiteflies: greenhouse whitefly – Trialeurodes vaporariorum Wstw., silverleaf whitefly – Bemisia tabaci Genn.; thrips: tobacco – Thrips tabaci Lind., rose – Th. fuscipennis Hal., western flower – Frankliniella occidentalis Perg., heterophagous – F. intonsa Tryb., greenhouse – Heliothrips haemorrhoidalis Bouche; mites: red spider – Tetranychus urticae Koch.; red greenhouse – T. cinnabarinus BoisdResults. Researches related to assessment of abundance of major pests for flower cultures and choosing methods related to determination of biological effectiveness of insectoacaricides were performed in flower orangeries of Leningrad Region on hybrid tea roses Rosa L., chrysnatemums Chrysanthemum L., gerberas Gerbera G. As a result, the algorithm for performing tests related to study of new insectoacaricides on flower cultures in greenhouses is developed. Methodology instructions that we offer for assessment of abundance of major pests for flower cultures of protected ground and determination of biological effectiveness of new insectoacaricides are intended for ensuring the uniform methodological approach to performance of registration tests of modern means to fight against these pests and opportunity for analysis of tests in various agroclimatic areas and obtaining a reasonable general conclusion and findings related to pesticides taking into account international requirements.
Show more [+] Less [-]Features of cultivation of new variety of potato Smolyanochka
2019
Larisa K. Сhehalkova | Aminat M. Konova | Anna Yu. Gavrilova
Relevance For the successful introduction of a new variety of potatoes into production, it is important to know the technological parameters of its cultivation, both for seed and food purposes. Methods Studies on the timing, planting schemes and doses of mineral fertilizers, providing maximum yield of seed and food tubers of a new potato variety Smolyanochka breeding Smolensk GOSHOS, were conducted in 2017-2018. Experience, observations and accounting were carried out according to generally accepted methods. The studies were conducted in the field short-term experience of the Smolensk Institute of agriculture (ex. Smolensk GOSHOS) on sod-podzolic medium-loamy soil with low humus content, weak acid reaction of the soil environment, high content of mobile phosphorus and high potassium content. Results Observations of the growth and development of potato plants of the Smolyanochka variety showed that the length of the growing season decreased from early planting to later. Planting schemes and fertilizer doses did not have a significant impact on the duration of development phases in all variants of the experiment. The density of the stem per unit area decreased by 5-18% from earlier planting dates to later and increased with an increase in the dose of mineral fertilizers to N60P72K90 by 12-28%, compared with the background of N32P32K32. The highest percentage of plant viral diseases during the growing season was observed with a more thickened planting scheme (70x25 cm) and increased with later planting dates. The maximum yield of potato tubers when grown for both seed and food purposes was noted against the background of mineral fertilizers at a dose of N60P72K90 when planted earlier. Post-harvest tuberous analysis of potatoes showed that all the studied variants of the experiment did not have a significant impact on the affection of potato tubers with fungal diseases.
Show more [+] Less [-]Factors of different quality seeds of green cultures of the <i>Brassicaceae</i> family
2019
Maria I. Ivanova | Anna I. Kashleva | Dmitriy N. Baleev | Alexander F. Bukharov
Relevance The phenomenon of the different quality of seeds of the sowing seed Eruca sativa, Diplotaxis tenuifolia, Brassica juncea by coloring was studied. Color heterogeneity is usually combined with the size and weight of seeds. Methods Research tasks: determine the number of seeds available for coloring in depending on different years of reproduction; identify the best fractions of morphological, physiological and productive qualities; to study the peculiarities of influence on the manifestation of ecological, agrotechnical and hereditary factors heterospermia. Results Seeds with a higher mass of 1000 were predominantly dark in color. The content of total nitrogen in light seeds was 0.2-0.3%, and crude protein was 1.3-1.9% more than dark-colored ones. The distribution of seeds of different colors in batches of different years of reproduction is shown. The best fractions of seeds by morphological, physiological and productive qualities are revealed. It has been shown that productivity and its components are higher in plants grown from seeds of dark color. The correlation of the parameters of seeds of different quality with the weather conditions of the vegetation period is noted. For all three crops, there was a steady increase in the proportion of light seeds with an increase in average daily air temperature (r = 0.59-0.94). The features of the influence of environmental, agrotechnical and hereditary factors on the manifestations of heterospermia are studied. However, the degree of influence of environmental, including agrotechnical factors in some cases turns out to be much stronger. The maximum proportion of dark (most productive) seeds is provided by a combination of optimal weather conditions, agrotechnical methods (early sowing, ripening), seed collection from shoots 1-3 orders of branching and use for sowing the dark fraction of seeds.
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