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Evaluation of the collection material of tomato varieties in dagestan in order to identify the most promising forms for tomato breeding
2022
P. M. Akhmedova | N. M. Velizhanov
Relevance. Tomato is one of the main vegetable crops and is grown everywhere in the open and protected ground in the North Caucasus region of Russia. Therefore, increasing the production of tomato fruits in the open ground is an important task for vegetable growers in the south of our country. There is too much dependence on imported seeds. To get rid of this, it is necessary to increase breeding activity, create domestic varieties and hybrids of tomatoes with high potential productivity, resistant to adverse environmental factors and adapted to local growing conditions.Methods. The research was carried out at the experimental site of the Federal Agrarian Scientific Center of the Republic of Dagestan, located in the Primorsky lowland, at an altitude of 17 m above sea level. Agrotechnics is generally accepted in the Republic of Dagestan for tomato culture.Results. The results of the research indicate that most of the studied varieties and hybrids belong to plants of the determinant type of early maturation with average stability, with large and medium fruits of flat-rounded and rounded shape, with medium and high yields and relative resistance to fungal diseases. According to the largest number of fruits on the first brush, four varieties differ: Topaz, Yana, Chelnok, Talisman (6.5-5.7). On the basis of the "number of fruits" on the second brush, almost all varieties are marked, which is of interest to breeding. On the basis of the "distance between the brushes" varieties Yana, Marti, Grant, Perst, Topaz – are characterized by a generative (shortened internodes) type of plant (12-16 cm). The source material also differs in the shape of the fruit – 8 samples have a rounded, 3 samples have an elongated oval, 5 samples have a flatrounded and 2 samples have a cuboid shape of the fruit. The color of the fruits in the technical phase of ripeness in the studied varieties and lines was with (8 samples) or without (12 samples) an intense green spot. In biological ripeness 12 varieties and lines have a red color of the fruit.
Show more [+] Less [-]Foliar biofortification of chervil with selenium and iodine under silicon containing fertilizer supply
2022
A. I. Moldovan | V. A. Kharchenko | N. A. Golubkina | E. D. Kekina | G. Caruso
Selenium (Se) and iodine (I) are essential elements for humans, and their deficiency is widespread throughout the world. In order to obtain a functional nutritional product with an increased content of these trace elements in the vegetative experiment, foliar biofortification of two chervil varieties with selenium (sodium selenate 10 mg/l) and iodine (potassium iodide 100 mg/l) was carried out without and against the background of the use of silicon-containing fertilizers Siliplant (3 ml/l). The combined and separate application of selenate, iodide and Siliplant increased plants’ biomass. Siliplant utilization increased the accumulation of iodine by 1.7-1.9 times, and selenium supply – by 2.2-3.1 times. A significant increase in ascorbic acid content was provided by the combined supplementation of iodine and selenium (1.25-1.27 times), iodine and silicon (1.46-1.87 times) and joint application of selenium, iodine, and silicon (1.31-1.73 times), while an increase in total antioxidant activity (1.3-1.4 times) was observed for (Se+I) and (Se+I+Si) treatments. High varietal differences in the responsiveness of plants to the selected treatments were manifested, particularly an increase of polyphenols accumulation under separate and joint treatments of chervil with iodine and selenium by 1.26 times in the cultivar 21-20, and the absence of a significant effect in the cultivar 24-20. Taking into account the adequate consumption levels (ACL) of iodine and selenium, 50 g of the resulting functional product can provide up to 79% of iodine ACL and up to 40% in selenium ACL.
Show more [+] Less [-]The effect of mulching materials on yield, biochemical composition and preservation of fruits of bell pepper
2022
E. V. Yanchenko | D. I. Engalychev | N. A. Engalycheva | K. L. Alekseeva
Introduction. The use of new covering materials is an important modern agrotechnique for cultivating of bell pepper in the open ground, which allows to minimize chemical treatments, increasing the yield, quality and preservation quality of vegetable products.The purpose of this work is to study the effect of various mulching materials (black polyethylene film and spandbond «Agrotex 60 perforated mulch») on the yield of bell pepper and also on biochemical composition of fruits and on their preservation quality in relation to the ripeness phase.Methods. The objects of the study were the fruits of two bell pepper hybrids of domestic and foreign breeding (respectively Nathalie F1 and Anetta F1), grown in the open ground using mulching materials. The experiments were laid according to standard methods adopted in vegetable growing. Storage was carried out in accordance with the methodological guidelines for conducting research on the storage of vegetables.Results. The use of mulching materials contributed to an increase in total yield and the yield of standard products in the crop structure, and also positively affected the accumulation of solids, vitamin C, monosaccharides, disaccharides and as a whole – the sum of sugars. The domestic hybrid Natalie F1 (74.9%), grown using black film as a mulching material, as compared to foreign hybrid Anetta F1 (71.3%), was characterized by better preservation quality. Significant differences in the chemical composition of fruits depending on the ripeness phase were revealed. Fruits laid for storage in full biological ripeness (red) were characterized by an increased amount of weight loss on all storage options. A positive effect on the storage persistence of bell pepper fruits with use of mulching materials on all variants of the experiment was also established.
Show more [+] Less [-]Creation of parthenocarpic lines of smooth and short type of cucumber
2022
N. N. Khomchenko | V. N. Shevkunov | V. N. Muliar | I. S. Pluzhnik | A. V. Kurepin
Relevance. Obtaining modern cucumber hybrids is impossible without the constant involvement of genetically new sources in the breeding process. One of the most important tasks in the issue of creating new heterotic cucumber hybrids is to obtain, and in the future, selection for hybrid crossings, parental lines with the most important economically valuable traits. Research is aimed at obtaining new short-fruited cucumber lines with a smooth type of fruit in order to obtain hybrids with their help that combine resistance to powdery mildew for the “pm” and “pmh” genes, tolerance to the zucchini yellow mosaic virus for the “zymv” genes in combined with high yields, the possibility of growing them not only in unheated film greenhouses, but also in glazed greenhouses.Methods. This article presents two schemes for obtaining parental forms using the example of creating four cucumber lines with a short, smooth fruit. Their comparative characteristics and description of new hybrids created with the participation of the studied linear material are carried out. Materials and methods. The main studies were carried out in 2013-2020 on the basis of the Pumpkin Cultures Laboratory of the Krymsk Breeding Center "Gavrish" of the Research Institute of Vegetable Breeding (Krymsk, Krasnodar Territory). The research material is F1 hybrids and selection samples of parthenocarpic cucumber with a smooth type of fruit of various origins (34 samples in total) from the NIISOK collection. The work used traditional methods for assessing selection-significant traits and modern methods of molecular labeling. The purpose of the work is to compare two schemes for obtaining short-fruited cucumber forms, to evaluate the created lines and hybrid combinations with their participation in terms of a complex of economically valuable traits.Results. The result of the work was the creation of new hybrid combinations based on the obtained lines, two of which are included in the State Register of Breeding Achievements. These are F1 Ministar (F1 1600/16) and F1 Promini (F1 1995/16).
Show more [+] Less [-]Evaluation of heterosis through reciprocal crosses of inbred cucumber lines (Cucumis sativus L.)
2022
A. A. Ushanov | R. A. Ulyanov | A. A. Mironov
The purpose of the study. The aim of the study was to determine the effect of heterosis in short-fruited parthenocarpic F1 cucumber hybrids by the main economically valuable characteristics, when grown using low-volume technology in industrial greenhouses of the Moscow region.Materials and methods. The object of the study is short-fruited parthenocarpic reciprocal hybrids of cucumber Druzhny (F1 I73514 x I74011), F1 I74011 x I73514 and their parent inbred lines I73514 and I74011 of selection by N.N. Timofeev Breeding Station LLC. F1 SV4097CV of foreign selection of Semenis company (Netherlands) was used as a control. The study was conducted in 2020-2021 in industrial greenhouses of the "V. I. Edelstein Vegetable Experimental Station" of the K.A. Timiryazev Russian State Agrarian University-Moscow Agricultural Academy in Moscow on low-volume hydroponics in peat bags. Parental lines and hybrid combinations were evaluated by earliness, early yield, yield, number of fruits per plant, average fruit weight and marketability.Results. According to the results of the study, F1 Druzhny (F1 I73514 x I74011) and the reverse hybrid I73514 x I74011 demonstrated high positive effects of heterosis in yield (MPH=19.4...22%; HPH=13.4...15.9%; CH=9.6...12%) and the number of fruits per plant (MPH=22.5...26.4%; HPH=12.5...15.8%; CH=11.7...15%). Negative indicators of the heterosis effect were noted for earliness from germination to flowering (MPH=-3.4...-1.1%; HPH=-6.5...-4.4%; CH=-4.4...-2.2%) and average fruit weight (MPH=-2.2...-1.3%; HPH=-5.3...-4.3%; CH=-3.4...-2.4%). The value of the degree of dominance indicates the presence of positive overdominance in the inheritance of such traits as yield (hp=2.7...3.2), the number of fruits from the plant (hp=1.5...1.9) and marketability (hp=1.6...2.3). Negative overdomination was observed in earliness from germination to flowering (hp= -2...-1.3) and average fruit weight (hp=-1.7...-1.2). According to the signs of earliness from germination to fruiting (hp =0) and early yield (hp=-0.1...0.5), intermediate inheritance was revealed. The Druzhny hybrid significantly exceeded the control hybrid SV4097CV in early yield, yield and number of fruits from the plant, and the reverse hybrid F1 I74011 x I73514 was at the control level, which indicates the prospects of growing these hybrids in the conditions of spring-summer turnover on low-volume hydroponics.
Show more [+] Less [-]Assessment of samples of common dill (Anethum graveolens L.) by a complex of valuable characteristics
2022
A. V. Piatrenka
Relevance. Among green and spice crops, dill occupies a special place in the human diet. The crop is widespread and is represented by varieties for various purposes. At present, dill varieties are subject to requirements for morphological (upright form of a bush, large leaf, etc.), phenological characteristics, high productivity of green mass and seeds, and quality. The biochemical composition is one of the main indicators of the quality of dill. Dill contains up to 90% moisture, however, even in that small amount of dry matter found in dill, there are many biologically impor- tant compounds that are necessary for the normal functioning of the human body. Therefore, when creating varieties, these indicators should be taken into account. For this, it is necessary to study and evaluate the parent material and identify promising forms for further breeding work in order to create varieties that have a complex of valuable characteristics. The purpose of the research is to evaluate the collection material of common dill according to a complex of breeding and valuable traits.Materials and Methods. The studies were carried out in the experimental field of the Horticulture Department of the Belarusian State Agricultural Academy in 2019–2020 on soddy-podzolic medium loamy soil. The experiments were performed in compliance with the agrotechnical require- ments for plant care throughout the entire observation period. The objects of research were breeding samples (26) of dill, selected at the Horticulture Department as a result of individual selection from the collection of varieties: Dukat, Sevastopol, Grenadier, Local Bulgarian, Mamut, Uzory, Salut, Almaz, Local Chinese, Alligator, Reshelie, Max, Dalniy, Rzheutsky, a local Krasnodar variety, Hoarfrost.Results. The article presents the results of a study of 26 breeding samples of common dill in 2019–2020. In the course of evaluating the collection material of common dill, highly productive samples were identified at different ripening periods. Differences in the content of dry matter, vitamin C, carotene, low accumulation of nitrates were revealed in the biochemical composition of common dill samples. According to the complex of traits valuable for breeding, samples were selected that can be used in further breeding work to create new varieties characterized by early maturity, high yield and product quality, as well as those having a longer term for the onset of the phase of industrial ripeness and a long period of shelf life.
Show more [+] Less [-]New tomato variety Agata Plus for open ground in the south of Russia
2022
B. N. Novikov | L. N. Novikova
Relevance. To increase the consumption of tomato and its diversity on the market, it is important to create new varieties that have economically valuable characteristics and are in demand among consumers. The aim of the research was to create a new determinant early-ripening variety of lettuce tomato, plastic, consistently productive, large-fruited, with dense pulp and high taste, tolerant to diseases.Materials and methods. The studies were carried out at the collection site of the Crimean OSS of the VIR branch (Krasnodar Region, Krymsk). The Agata Plus cultivar was obtained by crossing parental forms with subsequent multiple selections against a natural infectious background. As a mother, we used a salad variety of the Russian selection Agata – early maturing, undersized, with an average yield and fruits with good taste and average weight. The paternal form was a hybrid of the Dutch selection F1 Eploz, characterized by early maturity, medium height, medium leafiness, largefruited, dense pulp, consistently high yield and excellent taste of the fruit. The Agata tomato variety included in the State Register of the Russian Federation was taken as a standard.Results. Evaluation of breeding lines for a complex of biological, morphometric, economically valuable traits using individual and mass selections made it possible to obtain the best early ripening form of salad designation, marked by large fruit size, consistently high yield and good taste. In 2017, this form, called Agata Plus, was submitted to the State Variety Test, and since 2020 this variety has been approved for use.
Show more [+] Less [-]Influence of innovative plant growth regulators on physiological and biochemical parameters and yield of Solanum tuberosum
2022
A. A. Verizhnikova | E. G. Prudnikova
Relevance. Solanum tuberosum L. – it is one of the main food crops, therefore, the study of the influence of innovative growth regulators on physiological and biochemical parameters and plant yield is very important for the agro-industrial complex.Material and methods. The research was carried out on the gray forest soil of the Orel region in the conditions of a vegetation house on the basis of the farm Stebakov V.I. in the period 2020-2021. Potato of the Nevsky variety was used as the object of the study. Treatment with growth regulators was carried out by soaking Solanum tuberosum tubers in aqueous solutions of melafene (10-6%), Energy-M (10-3%) for 8 hours. When laying the experiments, the optimal amount of nitrogen (2.3 g), phosphorus (0.7 g) and potassium (3.1 g) was introduced into the soil for potato. Was determined catalase, peroxidase activity. The amount of hydroperoxides, an intermediate product of POL, was estimated by reaction with ammonium rhodanide, the content of malonic dialdehyde by reaction with thiobarbituric acid. The experiment was carried out three times. Statistical processing of the results was carried out by the method of one-factor analysis of variance using the computer program "MS Exel".Results. Studies have shown that there is an increase in the activity of catalase and peroxidase in all variants of the experiment compared with the control in leaves, in tubers – the activity of enzymes decreases. There was a decrease in the content of malondialdehyde in all variants of the experiment compared with the control. The analysis of the final productivity revealed that the total number of tubers on one bush of the potato in the variants with growth regulators changes slightly, while the mass of tubers increases, so the variant with the growth regulator melafen increased this indicator by 37% compared to the control.
Show more [+] Less [-]The influence of growth regulators on the ripening period, yield and quality of melon of the medium-ripened variety Osen
2022
N. B. Ryabchikova | M. S. Kornilova
This article shows the influence of growth regulators and methods of their application on the passage of the main interphase periods and growth processes during melon cultivation. The use of growth regulators is one of the promising directions in the cultivation of melons. In the acutely arid areas of the Volgograd Volga region, the use of mineral fertilizers for melon crops, in bagar conditions, was not effective, since there was an acute shortage of moisture in the soil. Therefore, the most effective and promising for creating favorable conditions for the growth and development of melon plants, with the possibility of leveling negative environmental factors, is the use of foliar treatments in the cultivation technology with growth regulators. The studied drugs of the new generation have a wide range of physiological activity. They are designed to influence the parameters of growth and development of plants as a whole in various phases, as well as their organs, as well as the parameters of plant response to environmental factors.Results. Studies have revealed the dependence of the growth processes of melon plants on the methods of using growth regulators, the length of the lashes in the variants of using growth regulators for processing plants during vegetation is longer compared to soaking seeds, with their maximum length in the Phytozont variant (plant treatment) is 12.7-51.3% longer compared to other studied preparations.
Show more [+] Less [-]Biotechnology methods in virus-free potato seed production
2022
I. V. Kim | E. V. Shishchenko | P. V. Fisenko | A. S. Chibizova | A. G. Klykov
Relevance. Plants of potato varieties are carriers of viral pathogens in a latent form. These viruses can be transmitted to clonal progeny of the carriers. The system of virus-free seed production facilitates the elimination of the viruses in seed potatoes and preserves the high productivity of potato varieties. The research goal was to develop a scheme for virus elimination in potato using biotechnological methods and to introduce this scheme in the production of virus-free tubers under the conditions of Primorsky krai.Material and methods. New promising variety Moryak (breeding number Pri-08-11-1), which was created in FSBSI “FSC of Agricultural Biotechnology of the Far East named after A.K. Chaiki”, was used as the research object. The mean yield of the new genotype is 34.1 t/ha, the potential yield is 40.1 t/ha. The dry matter content is 18.13-23.85%, the starch content is 12.10-17.24%, and the content of vitamin C is 17.46-23.12 mg/100 g. This variety has a high keeping quality of tubers (92.2-94.4%) and resistance to excessive soil moisture. Tissue culture and chemotherapy in combination with ribavirin (a concentration of 0.02-0.03%) and chitosan (0.01-0.1%) were used for virus elimination. Sprouts from the original tubers and plantlets were tested by EIA and qPCR for latent infection (PVX, PVY, PVA, PVS, PVM, PLRV).Results. A sequential increase in the concentration of ribavirin (from 0.02 to 0.03%) and chitosan (from 0.01 to 0.1%) and their alternation in different passages proved to be an effective method for virus elimination in plantlets. As the result of the research, the new scheme for the elimination of the most economically important potato viruses was developed and introduced, and virus-free seed material was obtained.
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