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Taxonomic evaluation using pollen grain sculpture and seed coat characters of 11 taxa of genus hibiscus, malvaceae, in Egypt
2011
El-Kholy, M.A. | Kasem, W.T. | Mabrouk, A.S.
Pollen grain morphology and seed coat characters of 11 cultivars belonging to two species of genus Hibiscus (Family Malvaceae) namely H. esculentus (H. Abelmoschus) and H. sabdariffa were investigated. This study was carried out using light microscope (LM) and Scanning Electron Microscopy (SEM). Pollen morphology of this genus is fairly uniform. Generally radially symmetrical apolar, mostly spheroidal, pantoporate. Seed exomorphic characters revealed four types of ornamentations; reticulate, ocealate, foveolate and ruminate. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) was employed to characterize those taxa. Thirty one bands of seed protein profiles have been constructed from the gel. The produced dendrograms were analyzed by STATISCA program using UPGMA clustering method showed a close affinity among the seven H. esculentus cultivars and the four H. sabdariffa cultivars.
Show more [+] Less [-]Anti-tumor activities and oil constituents of zingiber officinale, Zingiberaceae
2011
El-Swaify, Z.A. | Abd El-Kawy, A.M.
The phytochemical studies on Zingiber officinale rhizomes revealed that it contains traces of flavonoid, carbohydrates, tannins, steroles or terpenoids and it is free from alkaloids .Assay of essential oil of Zingiber officinale rhizome was also carried out. The alcoholic extract of the plant rhizome, was examined against five tumor cell lines, BHK-2, HEPG-2, MCF-7, HCT-2 and HEP-2, using SRB. assay. All cell lines were growth inhibited in a dose dependent manner after exposure to the plant extract. The antitumor activity of the plant extract using E.A.C. method, showed a high activity against mice tumor.
Show more [+] Less [-]Cellulase production by local bacteria isolated from taif in Saudi Arabia
2011
Nasr, S.A. | Abozaid, A.A. | Hussein, Y.A. | Al-Salemi, F.A
Among 20 bacterial isolates isolated from the soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia,two isolates had high efficacy in producing cellulase enzyme. They belonged to genus Bacillus (Bacillus 8 and 17). Some factors such as carbon source and wheat bran as a raw material, nitrogen source, pH and. incubation temperature were investigated. Results indicated that CMC and cellulose were the most effective as they enhanced cellulases production .Sodium nitrate and ammonium chloride were the best nitrogen sources for cellulases production. Initial pH 7.0 was found to be optimal for growth and cellulase production. Incubation temperatures at 25 - 40°C achieved high cellulases production by the two isolates.
Show more [+] Less [-]Cellulase productrion two fungal strains isolated from Taif in Saudi Arabia
2011
Nasr, S.A. | Hussein, N.A. | Abuo zaid, A.A. | Al-Salemi, F.A
Among 17 fungal isolates isolated from soil of El-hawia, El-hada, El-kaym and Karwa in Taif governorate in Saudi Arabia, two isolates showed high efficacy in producing cellulases enzymes. They were identified to be Altemaria altemata and Aspergillus wentii. Some factors such as carbon and wheat bran as a raw material, nitrogen, pH and incubation temperature were investigated. Results indicated that glucose and cellulose were the most effective as a carbon source while, urea was the best nitrogen source for cellulases production. Initial pH 5.0 and incubation temperatures at 25 or 35°C achieved high cellulases production.
Show more [+] Less [-]Physiological response of strawberry grown in root-knot nematode infested soil under different safety control applications
2011
Mahdy, M.E. | Midan, S.A.
The evaluate some safety treatments including: mulching, tunnels, natural yeast extract, antioxidant and two rhizobacteria Serratia marcescens and Pseudomonas fluorescens towards root-knot nematode Meloidogyne spp. on strawberry plant grown in naturally infested soil and consequently their probable effects on growth parameters, chemical components and yield of strawberry compared to untreated control. Results of this study confirmed that all used treatments, significantly reduced all related nematode parameters i.e. number of galls/root system; number of egg masses/root system as well as number of juveniles/250 g soil (either after 2 or 4 months) when compared to plants grown in the naturally infested soil only. The highest percentage of reduction in all related nematode parameters were noticed after 4 months. Mulching, S. marcescens, P. fluorescens and tunnels, played an important role in reducing the percentage of nematode parameters. Mulching and S. marcescens reduced the percentage of gall numbers/root system by 77%, followed by tunnels by 75% and P. fluorescens by 70%. S. marcescens reduced the percentage of egg masses/root system by 80% followed by P. fluorescens by 78% and mulching by 76%. Results indicated that the soil treated with mulching, also significantly reduced the percent age of juvenile numbers/250 g soil by 79% followed by S. marcescens by 73%. . Results revealed that all previous treatments significantly increased the early and total yield. Our results confirmed that, the rhizobacterium S. marcescens came in the first rank as a promoter of all related plant growth characters, followed by tunnels and mulching treatments. Results confirmed that also the antioxidants, are considered the effective one in enhancing the activities of peroxidase and phenoloxidase as well as T:S.S% and V.C followed by the natural yeast extract.
Show more [+] Less [-]Zinc and manganese sorption behavior by natural zeolite and bentonite
2011
Iskander, A.L. | Khald, E.M. | Sheta, A.S.
Understanding the sorption process in natural zeolite and bentonite is necessary for effective utilization of these minerals as nutrient adsorbents and on the other side for controlling release of plant nutrients. This research was undertaken to characterize the ability of natural zeolite and bentonite minerals to adsorb and release zinc and manganese. The potentials for sorption of these ions were evaluated by applying the Langmuir and Freundlich equations. The results showed that Langmuir constant (maximum adsorption (b mmol kg-1)) of both Zn and Mn were for bentonite zeolite While the [binding strength values (k in L.mmol1 for zeolite bentonite Zn in case of zeolite showed the lowest desorbed percentage particularly at high levels of sorbed Zn. Only, 74.7 % is readily extractable by DTPA after three successive extractions leaving, 25.3 % Zn retained by the mineral. In case of bentonite, 82.26 % of sorbed Zn is readily extractable by DTPA after three successive extractions leaving, 17.74 % Zn retained by the mineral. In case of Mn, 84.63% of Mn sorbed by zeolite is readily extractable by DTPA after three successive extractions leaving, 15.37% Mn retained by the mineral. In case of bentonite, 89.79 % of adsorbed Mn is readily extractable by DTPA after three successive extractions leaving, 10.21 % Mn retained by the mineral. This finding may reflect clearly the possibility of using natural zeolite and bentonite as a slow release fertilizer for Zn and Mn and on the other hand preventing soil pollution with heavy metals.
Show more [+] Less [-]Micropropagation of damask rose, Rosa damascena
2011
Alsemaan, T. | Albatal, N. | Almaarri, Kh.
This study aimed to establishing a protocol for producing in-vitro plants of Rosa Damascene. The culture was conducted at the Commission of Biotechnology in Damascus. Four types of explants were cultured on MS medium and some factors affecting culture were examined. The results showed that no viruses were observed, the lateral buds were superior over other explants, then the lateral microcuttings, after that, the apical microcuttings, and, finally, the shoot tips. The highest multiplication rate was observed at the hormonal combinations of (benzyl adenine BA 3mg/I with indole-3-acetic acid IAA 0.1 mg/1), and the highest elongation average were observed at ( IAA 0.1 mg/I with BA 2-6mg/I) or ( indole-3-butyric acid (IBA) 0.1 with (BA) 5-6 mg/I). The transferring was positively effective. The highest rooting percentage was observed when naphthalene acetic acid NAA or IBA were used. (Berlite: peatmoss, 1:1) was the best growing medium for hardening.
Show more [+] Less [-]Study of genetic relationship between some olive varieties in gellin gene bank
2011
Moustaf, N. | Hamed, F. | Lawand, S.
Olive tree (Olea europaea L.) is one of the most important fruit trees, there is a lot of varieties with the possibility of mislabeling, homogenouses and synonyms, which makes major troubles when having plant material for propagation and breeding programs. In this search, 12 olive varieties (Maw! Istanbul!, Khilkhali Khishen, Dan, Mniekri, Adkam, Doaibli, Mot, Khilkhali Saghir, Karamani Modabal, Mawi abo Shokeh, Sourani, and Abo Shokeh), cultivated in ACSAD Gene bank in Gellin, were characterized by ISSR molecular markers to determine the relationship between these varieties. Depending on quantitive traits the results indicated that these varieties are so close to each other's, ranged from 60% and 85%. It was noticed that Mneikri was apart from the other varieties in its traits, (polymorphic rate was 92.94%).
Show more [+] Less [-]Taxonomic study of two genus in lice linognathus africanus kellogg, and paine, 1911 bovicola, damalinia,ovis 1781 by scanning electronic microscope, sem
2011
Aljubran, S.A. | Alsaqabi, S.M.
This study presents the Mallophaga and Anoplura lice which affects farm animals (camels sheep and goats), a local area east of the Saudi Arabia of different ages, races, classes. Study the classification of lice was recorded as using the optical microscope and electronic scanning. The study shows scanning electron microscopy micro-structure of the species: Bovicola (Damalinia) ovis, Linognathus africanus and the study showed the installation of a portfolio of head lice, and the distribution of filament head, chest and abdomen, as shown over the centuries, the difference in the form of remote sensing and Swallows in thesexuallice, the first study in this area.
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