A field strain of pink bollworm Pectinophora gossypiella (Saund.) collected from Sharquia Governorate, Egypt was exposed to the selection pressure of the for-mulation of Bacillus thuringiensis subsp. Kurstaki, Dipel 2x under laboratory condi-tions. Resistance ratio attained 16-fold based on the susceptible strain after 14 gen-erations of selection. Study the response of Dipel 2x resistant strain to some insecti-cides indicated that there is no cross resistance to the conventional insecticides, es-fenvalerate, chlorpyriphos and thiodicarb or the bioinsecticides Ecotech and Agerin. These data may be emphasizing the possibility of rotation the Dipel 2x with these insecticides in pest control program of pink bollworm to manage resistance to Bt products.

Preparationof either Bacillus thuringiensis or Trichoderma harzianum  were used as seed coating or soil application for management of root-knot and root rot disease complex caused by the root-knot nematode, Meloidogyne javanica and the fungus Rhizoctonia solani on soybean plants. Number of galls, root galling, egg masses and disease severity were reduced sharply on plants treated with both biocontrol agents, either as seed or soil application compared with non-treated plants. Soil application with both biocontrol agents was the best method in reducing nematode and fungus disease severity. Plant growth parameters i.e. fresh shoot and root weight, dry weight and number of pods and bacterial nodules were markedly increased with all treatments of biocontrol agent either with seed or soil treatment compared to plants grown in infested soil with either pathogens alone or combined. Results show that plant survival was more affected when R. solani was alone or combined with M. javanica. Seed coating was considered the effective method in increasing survival plants. The biochemical analysis of treated plants with both biocontrol agents were affected compared to non-treated plants grown in pathogen infested soil. The biochemical parameters i.e. chlorophyll A and B, carotein, phenols and amino acids were enhanced in bioagent treated plants compared to non-treated plants