ABSTRACT   Iron deficiency anemia is considered one of prevalent patients in developing countries, whereas it is well known that wheat flour is deficient in iron, hence, in this study wheat flour (72%) extraction fortified with celery seeds and cinnamon bark meal individually as a natural sources of iron at levels 5, 7.5 and 10 g/100 g wheat flour and preparation of biscuit samples. Iron content in wheat flour, celery seeds and cinnamon were determined. Biscuit samples were sensory evaluated and baking quality tested. Total iron and available iron were determined in biscuit samples. Biological evaluation for experimental rats designed and histopathological examination was tested for heart organ of rats. The results showed that wheat flour, celery seeds and cinnamon contained from iron 1.98, 57 and 50 mg/100 g respectively. Total iron and available iron increased in biscuits samples by increasing celery seeds and cinnamon additives compared with unfortified biscuits (control). Sensory evaluation of biscuit samples showed slight decrease in color, crunchiness and appearance while odor and taste significantly improved by increasing celery seeds and cinnamon additives compared with control. Baking quality of biscuit samples indicated increasing in weight, while volume, diameter and thickness slightly decreased by increasing celery seeds and cinnamon additives compared with control. Biological evaluation revealed that mean values of hemoglobin, hematocrit, red cell count, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, serum iron and serum ferritin significantly improved after 8 weeks in groups rats fed on biscuits fortified with celery seeds and cinnamon compared with control. Histopathological overhaul declared amelioration in organ heart for groups rats fed on biscuits fortified with celery seeds and cinnamon compared with anemic control.

The interactions between dietary zinc, copper and iron and their effects on antioxidant enzymes activity [superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST)] were examined. Fifty-four rats weighting approximately between 80-100 grams were divided into nine groups (E1- E9). Each group containing six rats was fed on basal diet with varying amount of Zn (2.5, 35 or 175 mg Zn/kg diet), Cu (1.2, 6.3, or 25 mg Cu/kg diet) and Fe (10, 50 or 230 mg Fe/kg diet). The second group (E2) was fed a diet defi-cient in all studied elements (2.5mg Zn, 1.2mg Cu, and 10mg Fe per 1 kg diet). The results of E2 showed a decrease in SOD and CAT activity and increase GST in plasma erythrocytes and tissues. Moreover, plasma thiobarbituric acid reactive sub-stances (TBARs) and hydrogen peroxide produc-tion in E2 were found to be higher than those in other groups. The high activity of lactic acid de-hydrogenase (LDH) in the plasma indicated that cell membrane damage was occurred. This dam-age was accompanied by elevation of lipid peroxi-dation and in the same time by reduction of SOD and CAT activity. Supplementation of basal diet with the different concentrations of studied miner-als led to improve the activity of antioxidants en-zymes (SOD and CAT) and decrease in TBARs and LDH activity.