A considerable rhizopheric bacteria and mycorrhizae collectively known as plant growth promoting microorganisms (PGPM) have ability to induce acquire resistance in plant against pathogens and to provide benefits to their hosts. Grapevine viruses cause reducing yield and shortening the life span of infected plants in the vineyard. The current study aims to improve quality of Grape fan leaf virus (GFLV) infected grape plants via the soil inoculation with PGPM. Pot experiments were conducted under greenhouse during two seasons 2014/15 and 2015/16 in a Virology Greenhouse, Microbial. Dep. Fac. Agric., Ain Shams Univ. Cairo, Egypt. Grape cv. Flam grafted with GFLV infected stick and cultivated in inoculated soil with rhizopheric PGPB and mycorrhiza (VAM). GFLV was detected in infected leaves by DAS-ELISA. Plant growth parameters and chemical immune acquired resistance were assessment in GFLV infected grape cv. flam. The results were clearly indicated that PGPM inoculation in soil improved of plant growth in the second season (2015/16) compared with first season (2014/2015(. PGPM (Bacillus sp., Pseudomonas sp. and Serratia sp. isolates and VAM) improved quality of GFLV infected grape plants via increased plant growth parameters (leave number, stem diameter, plant length, and phosphorus and potassium components in leaves). PGPM induced acquire resistance in plant against GFLV; it was found that, significant increase of proline and SA contents in GFLV infected grape  leaves compared as healthy ones. The results revealed that chlorophyll a; b and carotenoids were significant decreased while inoculated PGPM in soil showed significant increase compared with healthy control ones. Expressed proteins and resistance enzymes (POD and PPO) of antiviral proteins were significant increase in PGPM application of GFLV infected grape growth related no inoculated PGPM ones. So that the current study recommended that the combination among VAM and PGPB soil inoculation improved quality of GFLV infected grape plant under greenhouse conditions.

The experiments were conducted to evaluate the effect of mycorrhizae (Glomus sp. and Gigspora sp.) application on the vegetative growth and yield of tomato plants (Alisa cultivar), and its relevance to the mineral fertilizers requirements as NPK during the seasons of 2015/2016 and 2016/2017. The experiments were conducted at Baloza Research Station, Desert Research Center, North Sinai Governorate, Egypt. The experimental design was a randomized complete block design with three replicates, every replicate included 4 treatments (75 and100% of the recommended mineral fertilizer only or combined with the mycorrhizae treatment). The results showed that mycorrhizae application significantly increased the vegetative growth characters of tomato plant such as plant height, number of shoots, number of leaves, stem diameter, fresh and dry weights and yield. There were no significant differences in the vegetative growth characteristics of tomato plants receiving 75% of mineral fertilizers in addition to mycorrhizae application as compared with plants receiving 100% of mineral fertilizers only, except for the fresh weight of plants. The plants were received 100% of the recommended mineral fertilizers combined with mycorrhizae treatment gave the highest values of vegetative growth characters and yield. Therefore, we recommended that, using of mycorrhizae could be affective to maximize the utilization of mineral fertilizers, in addition, the mycorrhizea is ecofriendly, economically and consid ered as one of the most important bio-fertilizer resources.