Tumor Necrosis Factor-α (TNFα) is a cytokine signaling protein which has an important role in the immune system regulation and affect female reproductive performance. The objective of this study was investigation the TNFα gene polymorphism and its potential effects in female buffaloes fertility. The DNA was extracted from the blood of 81 buffalo females and a 592 bp fragment contains the full coding region of TNFα-exon 4 was amplified by PCR which subsequently treated with RsaI restriction enzyme. The PCR-RFLP pattern showed that all the animals had fixed CC genotype and T allele was not detected. Sequencing of amplified fragment (GenBank accession No. KY885010) flowed by sequence alignment with GenBank database revealed that the river buffalo target sequence was homologues to cattle than goat, sheep, human and mouse on DNA and amino acids levels. Comparison of TNFα amplicon with homologues Bubalus bubalis records in the GenBank detected 3 SNPs in exon 4. Two of these SNPs were synonymous while the third located in the 3` UTR. Different effects of some of discovered SNPs on RNA cis-regulatory elements and hn mRNA and mature mRNA secondary structures were predicted.

Salinity is a stress factor affecting the production of crop in many regions.  Calcium can reduce Na+ transport to shoots in rice. Two greenhouse experiments were conducted in Faculty of Agriculture, Cairo University, Egypt, during 2015 growing season of rice to evaluate the effect of different calcium concentrations on survival percent along with uptake of Na+ and Cl- ions by two varieties of rice (Oryza sativa L.) differing in salt-tolerance. The first experiment was undertaken to study the effect of different calcium concentrations on survival percent of IR28 (salt-sensitive) and Nona Bokra (Salt-tolerant) seedlings which were transferred to salinized nutrient solution containing 0.5% NaCl and a variable calcium concentrations at 4, 40, 100 and 200 ppm; plants were grown up to 40 days. The second experiment investigated the effect of different calcium concentrations on growth, uptake and transport of Na+ and Cl- ions in the two rice varieties differing in salt-tolerance. The seedlings were transferred to salinized nutrient solution containing 0.5% NaCl and calcium ion concentrations at two levels, 4 and 40 ppm. Plants were harvested at 0, 1, 3, 5 and 7 days from salinization. The results indicated that the salt-tolerant variety (Nona Bokra) survived for more than 40 days under exposure to 0.5% NaCl when calcium concentration of the culture solution ranged from 40 to 200 ppm Ca++. The low calcium ion concentration (4 ppm) depressed the growth of plants at 5 and 7 days after salinization.  In Nona Bokra, the shoot had less sodium and Cl than the root. This implies that the salt tolerance of Nona Bokra may be attributed to the restricted translocation of Na+ and Cl- from the root to the shoot. Sodium as well as cloride content in the shoot of IR28 was more than twice that of Nona Bokra.  An adequate amount of Ca+2 tended to lower the salt injury caused by high levels of salinity in rice plants. The effect of calcium ion on salt tolerance varied greatly between Nona Bokra and IR28 varieties.

The effect of breederʼs strain (Hy-line Brown and Hy-line White W36) flock aged (28, 36, 46, 55, 66 weeks of age) and breeding season on some physiological measurements in egg, demand organs, dead supply organs and lymphoid organs were examined. At each age, 10 unhatched eggs from each genotype were examined on embryo weight percentage, Chorioallotoic  weight %, albumen pH %, yolk pH %, residual yolk %, pectoral  muscles weight %, hatch muscles weight %, heart, liver, lunges, spleen, burse weight percentage. There was a significant difference between layer breeder genotypes for lunges weight percentage the brown was higher (0.84) than the white (0.62). The breeding season had a significant effect on heart, liver, lunges weight percentages in winter higher than summer but yolk pH and residual yolk the summer higher than the winter. Breeder's age significant effect on embryo weight percentage, Chorioallotoic weight %, albumen pH %, yolk pH%, residual yolk %, hatch muscles weight % heart, liver, lunges and burse weight percentage.  It could be observed that the genetic differences among layer breeder strain and age of breeders can affect the hatchery performance. Additionally, handling of hatching eggs and incubation management can play a major role to overcome numerous hatching problems. In turn, increasing the production of table eggs and achieves the balance between supply and demand of that strategic commodity in Egyptian market.

The study aimed to identify the causes of early marriage for the children, his methods, and identify his effects. to determine the relationship between the age of the respondents at marriage,  the Attitude of respondents toward early marriage for the daughter / son and the demographic, economic and social variables. To Determine the relationship between the application of the respondents to family Planning methods and the demographic, economic and social variables. to determine the respondents Application gap regarding the application of family Planning methods, according to different regions of the study. to identify the respondents application gap of family Planning methods and the degree of preference for these methods. The study was conducted depending on the social survey method in the villages of Kafr Abu Najm and Awlad Eiladawy in Sharkia governorate on a random sample of 223 units represens 5% of the total number of women in each village, and col-lected data using a questionnaire by personal interview, starting from April until late of June 2016, data were analyzed using frequencees and percentages, alpha Cronbach's coefficient, weighted mean, Pearson correlation coefficient, contingency coefficient, the expense of applied gap. The study found a range of resalts including: that early marriage percentage (less than 18 years) in the field study amounted to 36.3%, showing that about 43.5%, and 49.8% of the respondents have negative attitud and neutral about early marriage for girls, while more than half respondents have neutral attitud about early marriage for the son by 60.5%. and prove the existence of a significant relationship between the age of the respondents at marriage and variables: the number of years of education for the respondents, the number of years of education for the husband, the age at first birth, the suitable age of marriage for girls, the suitable age for boys, The attitud toward the planning of the process of reproduction. The results indicate existence of negative relationship between the age at marriage and variables: the number of children, number of pregnancies, satisfaction with the difference between couple for the girl, satisfaction with the difference between couple for their sons. The study also found that 94.2 % of respondents have neutral and positive attitude toward regulating the reproductive process, while shows that the highest percentage of respondents neutral and positive attitude toward reproductive process. The results indicate that 49.8 % of the respondents have negative attitude for preference and 49.3 % for the application and there are gap between the application of the respondents to the methods for family planning and the degree of preference for these methods.

ABSTRACT   The research aimed to determine the level of participation of  Siwa Woman in the development of environmental industries, And determine the relationship between the independent variables and the degree of the level of participation of  Siwa Woman in the development of environmental industries, Determine the percentage contribution of variables moral relationship level of Siwa woman participation in the development of environmental industries, and to identify the motives of Siwa Woman in the development of environmental industries. This research was conducted in Siwa Oasis, It was selected sample of (256) respondents by 33.6% of the overall size, data were collected by using a questionnaire specially to achieve the research objectives, the statistical tools included percentage  Frequencies,  Pearson's correlation coefficient were used to data analysis.   The study concluded the following key results   The results showed that respondents with low participation in handmade carpets industry is 14.7%, While the respondents with medium participation is 22.3%, while respondents with high participation is 63%. The results indicate that the percentage ofrespondents with low participation in pickling olives are 12.3%, While the percentage of respondents medium participation is 15.9%, while the proportion of respondents with a high participation is 71.8 percent. The results showed that respondents with low participation in the manufacture of animal feed ratio is 17.2%, While the percentage of respondents medium participation is 15.2%, while the proportion of respondents with a high participation is 63.6 percent. Data suggests that respondents with low participation in the garment manufacturing Siwa is 7.3%, While the percentage of respondents participating medium is 9.9%, while the proportion of respondents with a high participation in the garment manufacturing Siwa is 82.8%. Results show that in the category of 7.8% total participation level low in the development of environmental industries, and 16.4% of them fall in the proportion of middle-class participation for the development of environmental industries, And 75.8% of the respondents fall in the category of high participation for the development of environmental industries in Siwa Oasis. Results show that the level of Siwa woman participation in the development of environmental industries was a significant relation at a level of 0.01 in all of: Capacity animal tenure, The degree of belonging to the tribal community, and the degree of adherence to tribal customs and traditions, Degree trend towards environmental industries, and the degree of exposure to sources of information on the environmental industries, As the value of simple correlation coefficient of Pearson: 0.225, 0.241, 0.217, 0.219, and 0.231, respectively, While the relationship was significant at the 0.05 level with age, While respondents 0.149 Pearson simple correlation coefficient values, and there was no significant relation to: the degree of education of the surveyed, the area of agricultural holdings. It turns out that the percentage contribution of these variables in explaining the contrast kidney respondents were significant at the 0.01 level And its contribution was all in the predictive ability and the other is 51.5%, of which 35.2% to the degree of exposure to sources of information on environmental industries, 9.5% to the point of belonging to the tribal community, and 5% to the degree of adherence to tribal customs and traditions, to 1.8% and the degree of the trend toward environmental industries. The results showed that the most important motives of Siwa woman in the development of environmental industries are: Increasing the family income to their participation in the environmental industries (89.5%), Help the family in the bride processing (83.9%), Out of the house to receive an education and to work in the environmental industries (76.7%).

This investigation was conducted at Barrage Station (BHRS), Horticultural Research, Kalubia Governorate, Egypt, to get some new and promising pea lines (Pisum sativum L.) using pure line selection method on the basis of genetic variability. Selection was performed between or within four pea populations of Master Bcultivar. The populations were collected from four different regions in Egypt and subjected to selection procedure during 2012/2013, 2013/2014 and 2014/2015 growing seasons. The investigation intended to study different traits of growth and yield and its components viz., No. of days to flowering, No. of first flowering node, plant length, No. of branches per plant, pod length, pod width, pod thickness, pod weight, No. of seeds per pod, weight of seeds per pod, No. of pods per plant, weight of 100 seeds, shelling percentage, pod yield per plant and seed yield per plant. The target of this study was to improve pea yield and its components as well as earliness of yielding. The analysis of variance revealed highly significant differences among genotypes for most of the studied characteristics, indicating ample scope of selection. Most characteristics showed a higher heritability coupled with higher genetic advance through two cycles of selection indicating that these characteristics are stable and can be improved through selection based on phenotypic observations. Twenty lines were obtained from selection program and were compared against the baseline populations in addition to check cultivar (Entsar1).Results showed significant differences among lines for most studied traits. Selection program resulted in genotypes or lines given symbols of H3, G7, N1, N3, G2, G1 and K2 that considered promising lines.  These lines have good pods traits, higher productivity and earliness of flowering. Simple correlation coefficient analysis revealed that greater emphasis should be given to the traits of No. of branches and No. of pods per plant along with pod length, pod width, No. of seeds and weight of seeds per pod for yield improvement.

Field studies were carried out to study the effect of the different four directions (East, West, North and South) and the middle of the orange trees on the occurrence of the citrus leafminer, Phyllocnistis citrella Staint. (Lepidoptera: Gracillariidae), attacking orange orchards during the period extended from the beginning of July, 2015 until the end of June 2016. Also, to determine the common predatory species occurred in navel orange orchards in Qaluobiya Governorate. The warmer months that have the highest temperature degrees and that months having the newly orange leaves, had the highest percentages of infestation by the pest. The obtained results revealed that, the South direction of the orange trees had the highest percentages of leaves damage by the citrus leafminer, P. citrella. Where, there were significant differences between the South direction (68.75%), in comparing with those of the West direction (64.42%), the North direction (60.58%), the East direction (54.58%) and the middle of the orange trees (45.38%). So, it is recommended to firstly begin the Integrated Pest Management (IPM) programs against the pest in this direction and at the suitable time. Moreover, the importance of the natural role of the common predatory species recorded in the orange orchard is obviously shown and must be encouraged and developed. The highest total numbers of all recorded predatory species were during July, 2015 (47 individuals) and April, 2016 (35 individuals). The recorded predatory species were the highest in the South direction (70 individuals), in comparing with those of the North direction (54), the East direction (50), the West direction (37) and the middle of the orange trees (20). True spiders were recorded as the most predators (114 individuals), in orange orchard and also in the same time of pest occurrence. The animal predatory species (true spiders and predatory mites) were the highest in their numbers (167 individuals), in comparing to the insect predatory species: Chrysoperla carnea, Scymnus sp., Coccinella undecimpunctata, Orius sp. and Metasyrphus sp., of lower individuals numbers (64 individuals). In general, from this study, it is also recommended to use the different predatory species such as: Chrysoperla carnea, Scymnus sp., Coccinella undecimpunctata and Orius sp., for P. citrella control firstlyfrom the South direction. Where, this pest controlcan be applied by mass rearing of these predators in the laboratory and releasing them in orange orchards against P. citrella or other related pests (such as the citrus aphids species and mealybugs). This approach is believed to be highly practical for use as one of the means of biological control with the frame work of suitable and safe IPM programs in citrus orchards in Egypt.

Experiments in this investigation indicated that the tomato cv. 035 is moderately resistant to Meloidogyne incognita (Mi) and Fusarium oxysporum f. sp. lycopersici (Fol) since it supports a low population of Mi nematode and retarded fusarial damage in single infections while the plant vigor was unaffected adequately. This resistance was decreased as increased of inoculum levels (from 100 to 2000 IJ2) of Mi and (from 102 to 1010 propagules) of Fol indicating that this resistance is mainly density-dependent. However, co-infection by both pathogens a lesser margin of simulative effect on root galling and wilt severity was occurred. Therefore, wounding by invading IJ5 of Mi proved to be unimportant for establishment of Fol wilt fungus. The results indicated also that, the sequence of infection by nematodes and fungus can predispose the host plant to attack by the other. As with Mi pre infection, nematode causes changes in host that predispose it to fungal attack leading to severe wilt expression. Likewise, pre-infection with for allowed not only more nematode penetration but also more nematode developed than in fungus. Accordingly, the present results led to the fact that, the interactions between Mi nematode and Fol wilt fungus are physiological rather than physical.

Three cancer cell lines, i.e. HEpG-2 (human liver carcinoma), MCF-7 (human breast adeno-carcinoma), and PC3 (Prostatic Small Cell Carcinoma) were used to determine the cytotoxic effects of the neonicotinoid insecticide (imidacloprid) and conazole fungicide (myclobutanil). Cytotoxicity was measured by neutral red incorporation (NRI) assay. The lowest concentration of the tested pesticides (0.5 μg/ml) was toxic. With the increase of the concentration up to 80 μg/ml, the Department of plant protection, Faculty of Agric., Ain Shams University, shoubra  Elkheima, Cairo, Egypt Department of Genetics, Faculty of Agric., Ain Shams University, Cairo, Egypt damage degree of the cellular form and size was more serious. The midpoint cytotoxicity value, (NRI50) for imidacloprid and myclobutanil for HEpG-2, MCF-7, and PC3 cancer cell lines were 110.5, 67.7 and 67.6 μg/ml and 38.12, 41 and 27.5 μg/ml, respectively. In general, myclobutanil was very toxic in the three cancer cell lines compared with imidacloprid.

Plants and plant by-products are now gaining attractiveness in treatment of bacterial infections and food preservation. The objective of this study was to assess antibacterial activity of some Egyptian plant and plant by-products against the locally pathogenic isolates from patients having infectious diseases in our country. Screening of antibacterial activity of ethanol, methanol and hexane extracts of some plants: grape leaves (Vitis vinifera), mulberry leaves (Morus alba), mallow leaves (Corchorus olitorius) and lemon leaves (Citrus limon) toward Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Salmonella sp., were investigated. Antibacterial activity was performed by the agar disc diffusion method. The ethanol and methanol extract of tested plant leaves showed promising antibacterial activities against both Gram-positive and Gram-negative tested bacterial isolates due to its great ability to extract those polyphenolic and biological active compounds from natural sources which effectively act against broad spectrum bacteria. Ethanol followed by methanol were found to be the best solvents of choice to extract natural products to get maximum health and medicinal benefits. The results revealed that the extraction efficiency increase with polarity increasing of the solvents, hence the highest extraction done with ethanol and methanol and the lowest extraction with nonpolar solvent n-hexanedid not exhibit any activity against all the tested bacteria. Irradiation at 5 and 10 kGy did not significantly affect the antibacterial activity of all tested plant leaves. Results indicate the potential of these plants for further work on isolation and characterization of the active compounds responsible for antibacterial activity and its exploitation as therapeutic agents