Guava die-back disease is a destructive disease. It causes serious yield losses and reduced guava fruits. Inspection of guava plant for die-back revealed that Beheira governorate had the highest frequencies of all isolated fungi from natural infected guava trees, followed by Alexandria, while Kafr El-Sheikh obtained the lowest frequency. Botryodiplodia theobromae gave the highest frequency of isolated fungi from natural infected guava trees during seasons 2015 and 2016, followed by Alternaria alternata, Alternaria sp., whereas Colletotrichum gloeosperiodes, Phomopsis sp., and Nigrospora sp. obtained minor frequency. The highest frequency of Botryodiplodia theobromae was observed at Beheira, followed by Alexandria governorate. Meanwhile, the lowest frequency was found at Kafr El-Sheikh. Pathogenicity test indicated that Botryodiplodia theobromae was the most pathogenic fungus. RAPD analysis of DNA by using three random primers exhibited five groups of DNA bands with first primer, six groups with second primer and five groups with the third primer and the overall similarities between isolates were arranged from 69 to 100% with P1, from 62 to 100% with P2 and from 33 to 100% with P3. The present study aimed to finding correlation between these isolates groups which resulted of RAPD, its pathogenesis and its geographical isolated locations. Ten isolates of B. theobromae represented ten locations of five governorates in Egypt were tested to evaluate the ability of pathogenesis in guava transplants cultivar Banaty at pots under experiments greenhouse conditions for artificial inoculation. The obtained results were exhibited that all tested isolates were clearly pathogenic to guava transplants and produced typical symptoms of guava die-back disease. Such isolates were differences of differed in their pathogenicity. The  ten isolates were coded according to their locations by three random primers (B.W.1, A.B.2, D.S.3, Q.K.4, A.A.5, B.N.6, K.Q.7, Q.S.8, D.B.9 and K.D.10). Isolate code No. Q.K.4 was the most virulent, followed by D.S.3 and Q.S.8, respectively which isolated of Qalyoubia and Damietta governorates. Meanwhile isolates K.D.10, A.A.5, K.Q.7 were moderately pathogenic, they were isolated from Kafr El-Sheikh and Alexandria, while A.B.2 and B.N.6 were the lowest pathogenic isolates isolated from Alexandria and Beheira governorates.

This study was carried out to investigate the effect of different group’s size on productive performance of growing rabbits. A total number of 108 APRI weaning rabbits, their average initial body weight were 560±10g. Animals were assigned to two groups: The 1st group housed by 4 rabbits in cage 60 cm2, while the 2nd group housed by 8 rabbits in cage 120 cm2. During the experimental period live body weight(g), daily feed intake(g) and mortality rate (%)  were recorded and daily body weight gain(g) and feed conversion ratio(FCR) were calculated at 5, 8, 10 and 12 weeks of age. After the end of the experiment (12 wk) 36 rabbits were randomly taken and slaughtered to calculate the measure carcass traits. These include total giblet (g), cervix part (%), front quarters (%), intermediate part (%), hind quarter (%) and the dressing weight. Also the economical efficiency (EE%) was calculated. Results indicated that: the average live body weight (LBW), daily weight gain (DWG) and daily feed intake (DFI) were not significantly influenced by different group size. The best DWG values were recorded for the group 2 (8 rabbits/ cage), by around 2.5% during the whole fatting period (5-12 wks). Daily feed intake was lower in group 2 than group 1 by around 6.20%, 2.00%, 0.31% and 2.83% at 5, 8, 10 and 12 weeks of age, respectively. Data revealed significant improvements in FCR for rabbits in group2 (P<0.05) than group1 for the period 5-8 and during the whole growing period 512 weeks. The mortality rate was lower in group 2  than in group 1. The best dressing weight value was recorded for the group 2 by around 9.2%.The economical efficiency (EE %) was improved in group 2. It is concluded that, increasing number of rabbits to 8 rabbits in cage could decrease the mortality %, increase the dressing weight that also lead to increase the economical efficiency.

Marjoram and Chamomile are an important exportable crops. Despite the importance of the previous crops in providing foreign currency to finance various investment. The crops are faced with many problems with importing countries. The study aimed at investigating the geographical distribution of Marjoram and Chamomile crops and to find important foreign markets and the possibility of exploring new markets. The study depends on the descriptive and quantitative methods. The study use of Boston model to determine the best countries that may absorb these crops.   The study reveals that Finland market absorbs about 7.6% of Marjoram exports about 7.9% of total exports. The model has also shown that European countries contribute with the highest rank share of the above crops exports. The study suggest to increase of the Arab countries store of the export of the above crops as well as some export countries such as Italy, France and UK.

This study was achieved at the Tissue Culture Laboratory of the Agricultural Genetic Engineering Research Institute, Giza, Egypt during the period from 2013 to 2017, Direct embryo initiation and callus formation of date palm cv. Sewi from immature female inflorescences have been achieved on modified MS medium supplemented with 4 mg l-1 Picloram plus 3 mg l-1 2 iP and 2 g l-1 PVP. Results also showed that BA at 0.5 mg l-1 produce the highest number of differentiated embryos/culture, while kinetin at 0.25 mg l-1 significantly increased the average number of adventitious shoots/culture. NAA at 1.0 mg l-1 induced the highest rooting percentage and micro-shoot length. On the other hand the best survival percentage during the acclimatization stage was observed with plantlets produced from IBA at 0.5 mg l-1 during the rooting stage.

Seventeen bacterial isolates have been successfully isolated and purified from soil rhizosphere samples collected from different agricultural areas Buhayra, Sharqia and Alqilyubia of Egypt, and screened for production of chitinase enzymes had the highest chitinolytic activities amongst those investigated. Isolate S3-C and S1-C were Identified by biochemical, physiological tests and 16s rRNA gene primer as Bacillus cereus S3C, Gene Bank NCBI accession MK185696 and Bacillus cereus S1C accession MK185697. The production of chitinase by B.cereus S3C and Bacillus cereus S1C was optimized using colloidal chitin medium amended with 1% colloidal chitin at 30°C after five days of incubation. B.cereus S3C had potential for cell wall lysis of many phytopathogenic fungi tested such as Fusarium oxysporum and Rhizoctonia solani Potato phytopathogenic fungi by in vitro antagonistic test. The addition of B. cereus S3C chitinase was more effective than that of B.cereus S1C in increasing the resistance of Potato plants infected with various Soil- borne phytopathogenic fungi.

This study was carried out to evaluate the antimicrobial activity of clove (Syzygium aromaticum) and thyme (Thymus vulgaris) ethanolic extracts. Different concentrations of ethanolic extracts were prepared and antimicrobial activity was determined against some bacterial, yeast and mold strains. The antimicrobial activity of examined extracts at different concentrations was determined against different cell population of tested strains and expressed as diameter of inhibition zone. Results showed that all clove and thyme ethanolic extracts were markedly inhibited the growth of all tested strains, however, the inhibition effect was differed with regard to the concentration of ethanolic extract as well as the type of tested microorganism. Generally, clove ethanolic extract had stronger antimicrobial activity rather than that of thyme ethanolic extract. Subsequently, clove and thyme are an extremely strong antimicrobial agent with usefull potential applications in food processing as a natural safe unsted of synthetic antimicrobial agents.

This experiment was implemented in the experimental farm of (Misr hytech seed co. Giza, Egypt) during winter growing seasons 2014 and 2015 to evaluate the inheritance of some cucumber vegetative and yielding traits using a complete diallel cross among five inbred lines. The results showed that the mean square of genotypes, parental inbred lines, crosses and parent vs crosses were significant for the studied traits. The hybrids P2xP4 and P2xP5 exhibited a useful heterosis relative to Mp and Bp for the fruit yield per plant and number of leaves per plant. The mean square of general and specific combining ability was significant for the studied traits. The higher GCA/SCA ratio than the unity of the studied traits indicating to the greatest role of the additive gene action in the expression of these traits. The inbred lines P1 and P 2 exhibited significant positive i g ˆ effects in the traits of number of fruit per plant and fruit yield per plant, so these inbred lines could be act as a good combiner for developing high yielding genotypes. The F1 hybrids P1xP3, P2xP4, P2xP5 and P4xP5 as well as the reciprocal hybrids P4xP1, P4xP2, P4xP3  and P5xP3 recorded a significant positive ij S ^  effect but the reciprocal hybrids recorded a significant rij effect. The results revealed to more than one hybrid distinct in some traits that could be utilized in the greenhouse cucumber cultivation and the future breeding programs.

Egyptian radish sterilized seeds were sprouted for 3 days using tap and saline water NaCl 2000 ppm to study sprout characters, chemical composition, protein fraction and safety aspects such as microbiological examinations and biogenic amines were investigated at 3 days old. The results indicated that sterilized seeds increased sprout growth compared with non-sterilized. Seeds sprouting increased in protein content, moister, ash and fibers while carbohydrate, lipid content and energy decrease in etiolated sprouts. Using saline water and seeds sterilization for sprouting increased albumin, globulin and glutenin fractionation but decreased prolamin compared with sprouts produced with tap water and without seed sterilization.  Using sterilization by 2% calcium hypochlorite of seeds before sprouting led to decreasing in total bacterial count compared with non-sterile seeds sprouts, the same was in total coliform, total yeast and total fungi counts. The sprouts product which washed with saline water was contain total bacterial count less than which washed by tap water. All sprouts under investigation were free from feacal coliform and all examined pathogenic microorganism under investigation like Staph. aureus, B. cereus and Salmonella spp.  Use sterilized seeds for sprouting caused big decrement on biogenic amines content of radish sprout. Radish sprout contain biogenic amines but it is lower than previous ranges, sprouts can be considered a safe food and germination of seeds either use tap or saline water.

Twenty two bacteria were isolated from soil samples local. were assayed for pectinolytic activity after optimization condition for pectinase production. isolate No. 22 showed high pectinase specific activity (91.4U/ml)  on glucose supplemented medium. the identification of the isolate revealed that it belongs to the Genus klebseilla bassed on morphological, biochemical characteristic, growth and molecular level 16s rRNA indicated that isolate was 99% similarity with Klebsiella oxytoca JKo3. Therefore, it was named Klebsiella oxytoca N22.

Two field experiments were conducted in two successive seasons; 2017 and 2018 at Bahi ElDin, Siwa Oasis, Egypt, to study the effect of four boron levels (0, 23, 46 and 69 ppm) as foliar application on yield and its components of five maize (Zea mays L.) hybrids (Single Crosses 124, 168, Three Way Crosses 310, 324 and 352). The experimental design was perpendicular strips plot with three replicates. Results indicated that effect of boron and hybrids on plant height, no. of grains/row, ear length, no. of rows/ear, ear diameter, 100-grain weight, grain, ear and stover yields (kg fed-1) were significant. Maize hybrid S.C. 124 produced the highest values of 100-grain wt., grain and ear yields per fed. Maximum no. of grains/row, ear length and stover yield fed-1 were recorded by TWC 324 hybrid. While, TWC 352 hybrid surpassed in no. of rows/ear and ear diameter. Foliar application by 69 ppm boron significantly produced more grains per row and ear, 100-grain wt. and grain yield/fed. Interactions were significant for all studied traits, except no. of grains/row and ear diameter. Thus, it could be concluded that applying 69 ppm boron as foliar for S.C.124 hybrid could be used successfully for improving maize productivity under Siwa Oasis conditions.