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IMPROVING THE MANAGEMENT OF WASTEWATER REUSE TO IRRIGATE LANDSCAPE
2020
Elshymaa Solima ELdeeb | Mahmoiud Hegazi | Osama Bedair
This study aimed to measure the effect of treated wastewater on the performance of pressure irrigation network components and compare it with domestic water. The experiments were conducted in Eastown and Lake View sites in the Fifth Settlement - New Cairo, at N = 30° 01' 14.4", E = 31° 51' 60.9" and N = 30° 02' 22", E = 31° 44' 31.3", respectively, and the experiments were done in (2019). The area under investigation was 155 m², divided into three plots (5×5 m) for spray irrigation, there areas were planted with turf grass (passpalm 10), and three plots (5×2 m) for drip irrigation, there areas were planted with shrubs (Lantana camara nana) and trees (Calistemon viminalis). By irrigation with two types of water: treated wastewater and domestic water with the use of two types of filters in the Lake View site (a screen filter and a sandy filter) and the use of one type of filters in the Eastown site, which is screen filter. The washing process was carried out manually and automatically. The results showed significant effects on the components of the network and on the general appearance of the vitality and shape of the cultivated surfaces. The most important results obtained were: • Emission uniformity for drip system in Lake View site, was higher when using automatic operation with treated wastewater by (5 and 6%) for on-line dripper and built-in dripper, respectively, than manual operation, while, in Eastown site emission uniformity was higher when using automatic operation with treated wastewater by (2.3 and 4.9 %) for on-line dripper and built-in dripper, respectively, than manual operation. • Distribution uniformity for spray system was higher when using automatic operation with treated wastewater by (5 and 5.1%) in Lake View site and Eastown site, respectively, than manual operation. • Clogging ratio in Lake View site by using manual operation with treated wastewater was (40 and 48%) for on-line dripper and built-in dripper, respectively. Clogging ratio using automatic operation was (20 and 25%) for on-line dripper and built-in dripper, respectively, while, in Eastown site by using manual operation with treated wastewater was (34.5 and 44.7%) for on-line dripper and built-in dripper, respectively, and by using automatic operation was (18.75 and 22%) for online dripper and built-in dripper, respectively. It was higher with manual operation than automatic operation and higher with treated wastewater than domestic water. • The concentration of total suspended solids was less by (93.6 and 97.9%) with manual and automatic operations, respectively in Lake View site, while, it was less by (50 and 60%) with manual and automatic operations, respectively, in Eastown site. • The turf quality index (color, density, ground cover) gave the highest degree when using treated wastewater with automatic operation, which is due to the nutrients in treated wastewater. • It is preferable to use sand filters before the mesh filters with treated wastewater to reduce the percentage of clogging with impurities instead of using only mesh filters.
Show more [+] Less [-]A COMPARATIVE STUDY OF THE COMPETITION THE MOST IMPORTANT WINTER CROPS, USING A LINEAR APPROXIMATE MODEL
2020
Moshira Al-Batran
Achieving the maximum productive economic efficiency to use the available resources is considered one of the most important objectives of the agricultural economic policy, and since the agricultural area is limited, therefore the decision to choose the appropriate crop for agriculture is subject to many considerations, perhaps the most important of which is the expected revenue of these crops, as it reflects many important factors that affect decisions farming at farms, such as prices and expected production of agricultural crops. The research problem is represented in the following question: How competitive and affect the cultivated area different crops, and do changes occur from one period to another? The research aims to compare the Competitive of the most important winter crops on agricultural land during the two periods (2014-2015), (2017-2018). The most important results were as follows: It was found that there are other factors that determine the cultivated area other than the farm prices for some crops, namely wheat, barley, onions, flax, and potatoes during the period (2014- 2015), barley, lentils, and lupine during the period (2017-2018). Low price elasticity and response was shown for chickpeas, lupine, fenugreek, tomatoes during the period (2014-2015), wheat, beans, sugar beets, persistent clover, onions, garlic, flax, tomatoes, and potatoes during the period (2017-2018). It was found that there were crops that responded more in the period (2017-2018) than the period (2014-2015), which were wheat, chickpeas, fenugreek, onions, flax, tomato and potatoes. It turns out that both chickpeas and lupine were exposed to competition from the rest of the crops during the two periods, and that wheat, broad bean, sugar beet, and flax did not compete for them in the second period, and on the other hand, clover, onions, and garlic continued to compete with other crops. Decreased Competition in the period (2017-2018) was shown in general compared to the period (2014- 2015). It was found that wheat and garlic were the most Interchangeability competitive, and tomatoes, broad bean, lentils and sugar beets were the most competitive crops of other crops, that lupine, fenugreek, and onions were the most competitive with other crops for the period (2014-2015). It was found that broad bean, clover, and clover Tahreesh were the most Interchangeability competitive, and barley, lentils, and potatoes were the most competitive crops of other crops, that flax, wheat, lupine, and tomato were the most competitive with other crops for the period (2017-2018). It shows the dynamics and instability of the agricultural map (with some being affected less) despite the convergence of periods. The research recommends, in order achieving maximum economic efficiency and production for the use of available resources, following: The agricultural policies applicable must be consistent with the changes that occur in the competitive of crops. Considerate the dynamics and instability of the agricultural map when setting agricultural policies. Factors other than prices must be used when determining the cultivated area of some crops, as a result of their lack of effect on some crops and their weak effect on others. Providing appropriate means (not necessarily financial support - Extension services, for example) to improve competition for some strategic crops.
Show more [+] Less [-]دور بعض المشاریع الغذائیة الصغیرة فی زیادة الدخل وتوفیر فرص العمل بمحافظة الفیوم
2020
Mona Shehata | Eman Hamed Elroby | Yasmin Aboseif
أصبحت مشکلة البطالة وانخفاض الدخول الحقیقیة من أهم المشاکل الملحة فی الإقتصاد المصری والتی یتعین مواجهتها لما لها من آثار سلبیة علی الإقتصاد والأمان الإجتماعی، إضافة إلی کونها أحد مظاهر أهدار الموارد المتمثلة فی القوی البشریة العاطلة الباحثة عن العمل وعدم توظیفها بما یساهم فی تحقیق نمو أکبر، وعلیه استهدف هذا البحث تحدید دور بعض المشاریع الغذائیة الصغیرة فی زیادة الدخل وتوفیر فرص العمل بمحافظة الفیوم وذلک من خلال عدة أهداف فرعیة وهی دراسة تطور المشاریع الصغیرة الممولة بمحافظة الفیوم، والتعرف علی قدرة بعض المشاریع الغذائیة الصغیرة محل الدراسة فی الحد من مشکلة البطالة وقیاس مستوى دخول العاملین. وقیاس العائد لتلک المشاریع. بالاضافة إلى التقدیر القیاسی لدوال الإنتاج والتکالیف لتحدید مدى کفاءة تلک المشاریع فی تحقیق التنمیة الإقتصادیة بمحافظة الفیوم. والتعرف علی أهم المشکلات والمعوقات التی تواجه تلک المشاریع محل الدراسة ومحاولة إیجاد الحلول والأسالیب اللازمة لحلها. وقد توصل البحث إلى عدة نتائج أهمها:- أن إجمالی عدد المشاریع الممولة وعدد المستفیدین من الذکور وإجمالی القروض بمحافظة الفیوم تتسم بعدم الاستقرار النسبی فی حین یتسم عدد المستفیدین من الإناث وتکلفة فرصة العمل بالاستقرار النسبی نظرا لانخفاض معامل الاختلاف النسبی والبالغ 48.84% ، 24.78% على التوالی. - بلغت قیمة المرونة الإنتاجیة الإجمالیة لمشاریع تصنیع الجبن البیضاء ومنشآت التخلیل نحو 1.28 ، 1.18 على التوالی ویعنی ذلک أن زیادة الموارد الإجمالیة بالدالة المقدرة بنسبة 10% تؤدی إلى زیادة فی إنتاج الجبن البیضاء والمخللات بنسبة 12.8 ، 11.8% على التوالی. وهذه النتیجة توجه بزیادة حجم تلک المشاریع وتوجه بإعادة مزج عناصر الإنتاج المستخدمة فی العملیة الإنتاجیة بما یحقق التولیفة المثلى منها والاستخدام الکفء لها للانتقال إلى مرحلة الإنتاج الاقتصادیة.- ویتضح تفوق حجم الانتاج الأمثل، وحجم الإنتاج المعظم للربح على حجم الإنتاج الراهن لمنشآت تصنیع الجبن البیضاء بمقدار 0.75 ، 2.44 طن على التوالی. کما یتضح تفوق حجم الانتاج الأمثل، وحجم الإنتاج المعظم للربح على حجم الإنتاج الراهن لمنشآت التخلیل بمقدار 5.02 ، 31.53 طن على التوالی.- أن نسبة إجمالی العائد إلى إجمالی التکالیف لمشاریع تصنیع الجبن البیضاء ومنشآت التخلیل حوالی 1.59 ، 1.36 على التوالی، وأن العائد على الجنیه المستثمر بلغ حوالی 0.59 ، 0.36 جنیه على التوالی. وبلغت نسبة التشغیل حوالی 0.63 ، 0.74 على التوالی ویعبر انخفاضها عن الواحد الصحیح على مدى اربحیة المشروع الإنتاجی.- وتبرز مشاریع تصنیع الجبن البیضاء والتخلیل أهمیتها فى استیعاب العمالة، کعامل جوهری ذا أهمیة فی النشاط الزراعی والمجتمع الریفی، حیث یعمل بها حوالى 124 ، 252 فرد على التوالی جمیعهم من ابناء القریة التی توجد بها هذه المنشآت ومن أبرز العوامل الکامنة وراء ارتفاع قدرة هذه الصناعات على استیعاب العمالة هو انخفاض تکلفة فرصة العمل نسبیاً بتلک المنشآت حیث قدرة تکلفة إتاحة فرصة العمل بنحو 8.35 ، 16.12 ألف جنیه على التوالی، کما بلغت نسبة متوسط إجمالی الأجور إلى متوسط إجمالی التکالیف المتغیرة بالمشروع نحو 26.6%، 35.4% على التوالی وبذلک تمتاز هذه المشاریع بارتفاع المساهمة النسبیة للأجور فی التکالیف المتغیرة، وبذلک تصبح هذه الصناعات قادرة على امتصاص العمالة الزراعیة الفائضة ، کما أن إقامة مثل هذه المشاریع فی المجتمعات الریفیة یؤدى إلى خلق فرص توظیف من ناحیة، وإلى زیادة دخول العمال الزراعیین من ناحیة أخرى
Show more [+] Less [-]STUDIES ON COMPATIBILITY, FRUIT SET AND FRUIT QUALITY BY DIFFERENT POLLINATORS IN ANNA APPLE CULTIVAR
2020
Mahmoud Maklad | Mai Anwar | نظمي عبد الغني | Nahla Awad
The apple (Malus domestica) is a member of the Rosaceae family. It is older than the rose in cultivation and is often referred to as the prince of the rose family. Pollen grains of Dorsett Golden, E25 and Ein Shemer were used to investigate their compatibility and incompatibility with Anna cultivar under the Egyptian climatic conditions during two successive experimental seasons of 2016 and 2017. The fluorescence microscope was used to determine the degree of pollen tube growth in style tissue after specific pollination treatments. Degree of self and cross compatibility and or incompatibility were determined, initial, and final fruit set percentage were calculated. Pollination treatments revealed that, pollens of the four apple cultivars exhibited high rate of the viability after 24 hr. in T.T.C test. Growth of pollen tube of the tested cultivars exhibited different pattern of compatibility, the pollen tubes of Dorsett Golden and Ein Shemer cultivars showed different levels of cross-compatibility when fertilized in Anna style tissue. The pollen tube of E25 cultivar grew slowly with heavy deposition of calluses along to the tube where its growth stopped in the lower part of Anna style four days after pollination. Therefore, they need pollinizer cultivars as for good fruit set. On the other hand, Anna self-pollination seemed to be self-incompatible and recorded lowest initial of fruit set percentage in the first and second seasons. The combinations of Anna × Dorsett Golden exhibited maximum percentage of initial and final fruit set in the two seasons followed by Ein Shemer pollens in both seasons. Insignificant differences were noted in fruit weight and fruit volume among all crosses. The highest acidity was found in hybrid Anna × Dorsett Golden in except in the first season TSS values of these crosses were not high and ranged between 11.83 % and 12.83 %.
Show more [+] Less [-]EFFECIVENESS OF SOME AROMATIC OILS TREATMENTS ON QUALITY CONSERVATION OF PEPPERMEN FRESH CUT HERB UNDER COLD STORAGE
2020
Ahmed Abdel Hamid
The effect of lavender, lemongrass and thyme volatile oils as a supplementary cold storage treatments on quality preservation of fresh cut herb of Menthe piperita L. , during storage for 15 days at 5°C and 7°C followed by 5 days at 20°C, as a marketing condition simulation, was evaluated during 2018 and 2019 seasons. Physical properties, chemical constituents, respiration rate and volatile oil contents were recorded. The results illustrated that discarded herb %, weight loss %, and external appearance (scale) were greatly affected by both factors of the study. The great effect in this respect was obtained with storage at 5°C than 7°C and the effect was also continuous during marketing period at 20°C. An evident decrease in discarded herb % and weight loss % were obtained due to the applied treatments, whereas external appearance values were higher with applied treatments than control during cold storage durations and marketing period. Aromatic oils treatments with 0.50% of lavender, lemongrass and thyme oils were effective than 0.25% of the three aromatic oils. However, chemical constituents i.e total chlorophyll, L-ascorbic acid and total phenols of fresh cut herb were scored highly increases by storage at 5°C than at 7°C storage conditions. Furthermore, The richest chemical constituents mentioned above were obtained with 0.50% of the three aromatic oils treatments and with superior for marketing durations for 5 days at 20°C. On contrary, total acidity of fresh cut herb recorded the minimum values with applied treatments than control (except the treatment of 0.25% thyme oil) during all cold storage durations and marketing period. On the reverse , electrolyte leakage and respiration rate values were controlled and minimized with the applied aromatic oils treatments under 5°C or 7°C cold storage conditions and followed by 5 days at 20°C as a marketing period. The decreases of both electrolyte leakage and respiration rate of fresh cut herb either with cold storage or due to the applied treatments are considered a good indicators to increase storage longevity and high quality. Meanwhile, volatile oil was greatly affected by both cold storage conditions and supplementary refrigeration treatments with lavender, lemongrass and thyme aromatic oils. Conclusively , it could be concluded that the peppermint fresh cut herb can be treated with lavender, lemongrass and thyme aromatic oils before storage at 5°C for 15 days , handling and marketing under 20°C to conserve the quality of the herb.
Show more [+] Less [-]Potential the biological or chemical control of lettuce white rot and maintain productivity
2020
Samuel Allah | Ahmed Abou El-Yazeid | hany gamal | ahmed bondok
The experiments were executed on the autumn and the winter of (2016 /2017, 2017/2018) seasons at Qalyub area, EL-Qalyubia governorate, Egypt. The main objective for this study to find out the efficacy of several biological and the chemical controls on growth and productivity of lettuce crop and management on white rot disease caused on Sclerotinia sclerotiorum. Thus, four treatments were applied as follow 1- the control (water application),2- Trichoderma asperellum, 3-Salicylic acid and 4- Calcium Chloride, which were evaluated and compared for the three fungicide as follow : 1- Iprodione , 2- Tebuconazole with Fluopyram and 3-Tebuconazole at the doses (85 & 100 ; 200 &100 and 50 &100 g or ml / 100L-1 respect.) . Which they tested for their ability for increasing the lettuce crop productivity and suppress mycelial growth of S. sclerotiorum pathogen by treatments were sprayed on two times on plants at (15-19 BBCH) stages by using backpack sprayer by 250 L./Fed.). Results indicated that, Iprodione applications significantly gave the highest indications of total and the Marketable yield in compared with the other experimental treatments for both seasons. Moreover, the two times of sprayes by Iprodione and Salicylic acid treatments had significantly lowered the disease incidence and disease severity and increasing the control efficiency in the both seasons. Whereas, the other treatments, Tebuconazole with Fluopyram or Tebuconazole and Trichoderma asperellum had a moderate effecacy on the lettuce crop productivety or the disease severity and incidence of Sclerotinia sclerotiorum in compared to the control treatment. In the contrast, application by Calcium Chloride had a low effect on the average yield or final yield as a mass and marketable plant in the both seasons and had insignificant effect on the disease severity or incidence of S. sclerotiorum despite low to medium recovery following applications. Finally, the control treatment was the lowest.
Show more [+] Less [-]MONITORING OF COUNTERFEIT ABAMECTIN PESTICIDE PRODUCTS IN EGYPT.
2020
Gehad Khattab | Walaa Abdelghany | Mohamed Abdelmegeed | Ibrahim Attalah
The monitoring of the counterfeit situation of a pesticide widely used in Egypt (abamectin) was done through the present study as seven samples of Abamectin formulations were collected from the Egyptian market. Packaging and labels were checked and analyzed by HPLC to determine the active ingredient content present in these samples. GC-MS and FTIR were used for additional analysis and detection of other active ingredients in the formulation.Counterfeit pesticides were studied in Egypt by doing questionnaires for all workers in the pesticide system (farmer - trader - researchers in the pesticide field) and the results of these forms were analyzed to show the most dominant pesticides in Egypt, in addition to random purchase of pesticides from the Egyptian market and the most number of samples Is from (abamectin pesticide) (7 samples).To obtain this number of abamectin formulations, 58 different pesticide formulations (16 different active ingredients) were collected from the Egyptian markets, and the share of abamectin was 7 samples (one active substance), representing 12.1% of the total tested samples. The obtained results showed that Examination of Packaging and indicated that three samples were not registered through Egyptian Agricultural Pesticides Committee thus representing 42.86% of the total tested samples. The non-registered products are Komaktin Gold Plus, Super Vertimic and Abamectine Strela. Three samples have registration number as other formulations already registered in Ministry of Agriculture which are Abamectin Super, Farmactine and Abamectin power. The last product (Tinam) has the correct registration number (no.1391) and it is the same number used to register it in Ministry of agriculture. The percentage of the active ingredient in 5 samples was less than the acceptable limits, The samples were (Abamectin super, Komaktin Gold Plus, Abamectin Power, Super vertimic and Abamectine strela) active ingredient content was 1.416, 0.64, 1.01, 0.2 and 0.12% respectively. One sample (Farmactine) didn’t contain Abamectin, and the sample (Tinam) was within the acceptable limits (1.53%). GC-MS used to determine the presence of any other materials or active ingredients within the samples. The result showed that Abamectin Super sample contains (Lambda cyhalothrin at R.t. 29.512 min), Farmactine sample contains (Fenpropathrin at R.t. 28.634 min), Abamectin Power sample contains (Diazinon at R.t. 16.088 min and Cypermethrin at R.t. 26.554 min) and Super Vertimic sample contains (Fenpropathrin at R.t. 23.916 min and Lambda cyhalothrin at R.t. 34.85 min). Three products Komaktin Gold Plus, Tinam and Abamectine Strela didn’t contain other active ingredients.The physical properties of the studied samples before and after storage were determined through emulsion characteristic test and four samples (Abamectin super, Farmactine, Abamectin Power and Tinam) showed good emulsion characteristics before and after storage. Super vertimic and Abamectine strela samples showed poor emulsification characteristics before and after storage. Komaktin Gold Plus showed good emulsification before storage but poor emulsion characteristics after storage.
Show more [+] Less [-]HISTOLOGICAL CHANGES ACCOMPANYING ADVENTITOUS SHOOTS FROMATION OF FRAGRAIA X ANANASSA IN VITRO LEAVES
2020
Seham Abed
A tissue culture experiment was conducted to attain the adventitious buds initiation and growth from Fragraia x ananassa cv. Festival in vitro leaves. The leaves were excited from shoot propagated in vitro and cultured on Murashige and Skoog (MS) medium supplemented with 4.50, 6.75, and 9.00 mmol l-1 thidiazuron (TDZ) for six weeks. Then explants were transferred to MS medium (salts and vitamins) free plant growth regulators for 8 weeks. This study included the histological and morphological responses of strawberry leaves with TDZ treatments in relation to some biochemical components. All TDZ treatments resulted in adventitious shoots development on leaf explants without callus formation. Growth in 6.75 mmol l-1 TDZ led to an increment in the most morphological parameters compared to the rest treatments. The results showed that 6.75 mmol l-1 of TDZ treatment significantly increases the number of shootlets per explant (5.0), total shoot (51.3), the number of leaves/explant (12) as compared with other treatments while the rest of parameters (the survival percentage, shoot length (cm), number of leaves/explant, root length (cm), and number of roots/explant) did not show any significant differences. Accumulation of chlorophylls, total sugars, and total soluble phenols was enhanced by 6.75 mmol l-1 of TDZ. The histological observations showed that adventitious shoots initiated from some parenchyma of ground tissue in the midvein zone of the explant, whereas the parenchyma mesophyll and the epidermal cells did not involve in this process. The ontogenesis of adventitious shoots was described by successive stages with the following distinguishable anatomical structures: meristemoids, bud primordium, shoot apex with leaf primordial, branching of adventitious shoots. After four weeks of culture, cell dedifferentiation was recorded in the midvein parenchyma and forming small groups of divided cells called meistemoid centres. These centres forming new meristematic masses embedded in the ground tissue of the main vein. After six weeks, further development of these masses resulted in the formation of bud primordial with normal organized shoot apical meristems and leaf primordial and arising from the explant as small protrusions. After two weeks from subculture on MS free TDZ, the adventitious shoots continue to elongate with forming new lateral branches and clearly observed on the adaxial side of the explant. This procedure provides a simple and rapid approach to regenerate strawberry plants via direct organogenesis.
Show more [+] Less [-]Evaluation of mint and sweet basil herbs production integrated into the Aquaponic Tilapia production system
2020
Sabah Salama | Awad Kandil | Mohamed Elshenawy | Mohamed Abdelbaki | Mohamed Abulseoud
The impacts of climate change combined with water shortage and the need to increase food production to meet increasing population stimulate the implementation of Aquaponic systems not only on food scale but also on some medicinal plants in agriculture production. The current experiment was conducted at the Institute of Graduate Arid Land Agriculture and Research Institute (ALARI) at Ain Shams University, Egypt, to test sweet basil and mint development during the summer seasons of 2017 and 2018 under urban conditions through the aquaponic system. The objective of the study was to investigate the use of aquaponic compared to the chemical nutrient solution (control) as a nutritional source combined with two plant densities (6 and 8 plants / m²) in the design of complete randomized blocks for evaluating basil and mint growth. The density of the Nile Tilapia was 100 fish / m3 in the aquaponic facility. The yield characteristics, the nutrient (N, P and K) and the oil content (percent) of basil and mint, have been measured in. Reveled results indicated that basil plant had a higher capacity for removing NH4, NO3, P and K from fish rearing water than mint while plant density 8 plants/m2 had a higher capacity compared plant density 6 plants / m2 resulted enhancing the quality of fish rearing water led to increase the fish yield (the final and gain tilapia yield). The chemical nutrient solution provided higher plant height, fresh and dry yields of basil and mint than the aquaponic solution, as well as N, P, K, and oil content (percent) of basil and mint plants in both plant density (6 and 8 plants / m²) and in both seasons. The composition of aquaponic water as a source of nutrition was not sufficient to meet the requirements for basil and mint nutrients. The highest plant height, N, P, K, and oil content results were reported for the treatment of chemical nutrient solution + plant density 6 plants / m² while chemical nutrient solution + plant density 8 plants / m² had the highest fresh and dry yield of basil and mint. The need for increasing the food production under climate change impacts, the use of aquaponic systems for sweet basil and mint production provided food production (fish and vegetables) as well as the medicinal plants at the same time protecting the environment by avoiding the use of chemical fertilizers.
Show more [+] Less [-]Genetic Improvement of Calendula officinal through Mutation Induction using Gamma Irradiation and Chemical Mutagens
2020
eman elmenbawy | Eman Fahmy | Sawsan Elateek | Nahla Awad
Calendulais one of the basic plants of ornamentals. It is used in various purposes as cut flowers and in gardens. They are rich with active compounds such as chlorophyll a and b, phenols, flavonoid, and carotenoids. The present study focused on mutation induction in order to improve the morphology of the plant which can increase the turnout of its market. In order to examine which mutagen will make the most noticeable and permanent improvement of the plant. For the mutagens, seeds were treated with three doses of gamma ray (2500, 5000, 7000Gy) and two chemical mutagens; colchicine and EMS; which were applied at concentrations of 1000, 3000, and 10000 ppm. With the aim to measure the plant improvement, we measured the plant morphology, its active compounds and protein profiling before and after treatments with both γ-radiation and chemical mutagen to see which one has the most effective imprint on the plant. The morphological parameters data showed the highest plant high by the two doses; gamma dose of 7000 Gy and EMS concentration 10000 ppm, while the lowest dose of colchicine (1000 ppm) made the most impact on the plant height. As for the highest number of leaves/plant, it was obtained at 5000 Gy and 10000 ppm of EMS, while 1000 ppm of colchicine had the same effect. But the number of flowers/plant was not affected by the gamma radiation and colchicine but increased significantly by 10000 ppm of EMS. Surprise, the flower diameter was not affected by EMS while decreased at 2500 Gy and 3000 ppm of colchicine. Considering the importance of flower shape, all treatments either gamma radiation or chemical mutagens showed noticeable changes. Whereas, the biochemical parameters measuring the active compounds, both Chl-a, Chl-b and carotenoids contents increased at 5000 Gy, while flavonoid increased at 2500 Gy. Although, all doses of gamma ray eliminated the phenols content. While the lowest used concentration of colchicine (1000 ppm) increased both of Chl-a, Chl-b, flavonoid and carotenoids, even though the 10000 ppm of colchicine increased the phenol. On the other hand, the highest concentration of EMS (10000 ppm) increased Chl-a and Chl-b while carotenoids, flavonoid and phenol increased at 3000 ppm of EMS. The protein-profile analysis showed fluctuation in the gene expression. The highest performance appeared at 7000 Gy of gamma treatment,1000 -3000 ppm of colchicine, while the same result was obtained by EMS at 3000 ppm.
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