The present work is aimed to isolate, purify, identify a newly isolate Rhizobium fabae from different regions in Egypt then, produce and optimize Poly-β-Hydroxybutyrate polymer (PHB) production by the selected isolate Rhizobium fabae F44 using a statistical approach of response surface methodology (RSM). Rhizobium fabae F44 isolate was picked up from Sharkia Governorate in Egypt and then identified by the 16SrRNA gene as Rhizobium fabae. Eleven different variables which affect the production of PHB polymer were screened by Plackett–Burman statistical design approach. Maximization of PHB production was adjusted by the terms of The Face Central Composite Design (FCCD) of RSM and assessed at three coded levels (–1, 0, +1). One way Anova was used to statistically analyze all obtained results in relation to post hoc multiple comparison analysis performed by Tukey’s HSD. Appulses of nutritional and physical factors (two carbon sources, two nitrogen sources, mineral salts, pH, temperature, incubation time, inoculum size and agitation rate) were studied using Plackett-Burman design method. 58 isolates were obtained from the different 9 governorates with 100 % infection plant technique. The 16SrRNA showed a similarity of 99.56 (%) to Rhizobium fabae F44. Out of all tested significant media components, sucrose, yeast extract, agitation rate had the highest significant effects on the response as for PHB production, with confidence level > 98% and were further optimized using FCCD. Predicted Maximum PHB production was observed as (78.51%) which in near the mid-point (0) values (concentrations) of sucrose which reached (25 g/l) and yeast extract of (0.5 g/l) for 48 hrs. at 150 rpm agitation rate. The observed experimental value reached 87.5u/ml was very close result to the predicted one validating the model. So, Response Surface Methodology is an effective statistical approach which can substitute the use of one variable at a time approach due to its adequacy and efficiency to optimize PHB production by Rhizobium fabae F44.

The present study was conducted to prepare nano-chitosan particles (CN) by ultrafine grinding  from crude chitosan powder (CC) using a ball mill with 130 numbers of zirconia beads in range from 0.5 to 1.5 mm diameter (75 beads 0.5 mm diameter, 30 beads 1.0 mm diameter and 25 beads 1.5 mm diameter). In addition, physical and rheological properties of chitosan solutions were determined. The results revealed that ultrafine grinding has effectively milled the chitosan particles to nanoscale. Prepared chitosan nanoparticles were characterized by  devices  X-Ray Diffraction (XRD), Zetasizer particle sizes and Zeta potential of chitosan particles (after milling for 30, 60 and 90 minutes). The particle size of nano-chitosan was distributed in a range of 250-600 nm. with a polydispersity index use as an anti-oxidant and antimicrobial with reducing the amount used to deliver the desired purpose. Additional research is required to further investigate the potential value-added utilization of these chitosan derivatives in improving the quality and safety of some food products.

As the impact of upcoming climate change scenarios on livestock feeding security (quality, availability, and price), applying alternative feedstuffs is a must. Fattening calves considered as a most process related to traditional feeding system. In the present study we purposed new feedstuffs for fattened calves consisting citrus pulp silage, wheat germ, barley sprout and molasses. Within the above feedstuffs we using mechanical, chemical, and biological treatments. Moreover, the present study evaluates the production performance of 14 male cross Friesian calves (7 per each group) for adding 10ml/calve/day probiotic ZAD in treated group. Weight data where used to calculate Average Daily Gains ADG, nutrients digestibility, boneless meat percent, and feed conversion rate. From our results applying probiotics (ZAD) increased significantly (P<0.05) ADG in the hall fattened period by 1.011±0.107 kg/calve/day and 0.886±0.107kg/calve/day, also, increased the percent of boon less meat from 51.097±1.140 % and 44.410±1.518% in the treated control group respectively. Applying probiotic increasing significantly (P<0.05) digestibility of nutrient detergent fiber and acid detergent fiber by 42.853±7.092 and 54.010±3.774 in treated group compared with 25.180±7.902 and 37.679±3.774 respectively. Based on the results of the present study concluded that the effect of probiotic treatment on feed conversion rate of intake nutrients showing significant different (P<0.05) in Total Intake of Digestible Nutrients TDNI was higher in control group (4.579) compared with treated group (3.305). However, Dry Matter Intake DMI and Crud Protein Intake CPI tended to be higher in control group compared with treated group. In conclusion applying probiotics in agriculture byproduct silage in presence of sprout, molasses and wheat germ results in improving in nutrients digestibility, growth performance, and feed conversion rate of fattened calves. Based on our results we recommend such feeding system as a solution in calves' meat production to facing the upcoming climate change scenarios (i.e. direct and indirect effects on livestock sector.

Noodles were prepared by incorporation varying blends (0, 20, 40, 60 and 80%) of rice flour in replacement of wheat flour. Noodle samples were evaluated for their proximate composition, scavenging activity, sensory properties and cooking quality characteristics. The proximate composition of uncooked noodles varied significantly, with increasing rice flour level that replaced wheat flour , a gradual decreases in moisture , ash , crude fiber and protein contents in noodle pastes were occurred , their values ranged between 4.02-5.33% ; 1.11-5.22% , 0.65-0.82% and 8.51-12.81% ,respectively. However, an increases in fats, total carbohydrates and energy value were take place ranging between 4.62-8.91 % , 71.11-76.74% and 377.64 - 421.43 kcal/100g, respectively .The antioxidant activity ranged between 22.2% for wheat based noodle (100% wheat flour) to 36.8% for sample containing 80% rice flour as scavenging activity for free radicals. The overall acceptability of wheat-based noodles (WF: RF 100:00) were rated superior for their sensory preferable characteristics. Among the blended flour noodles, the noodles incorporated with up to 40% rice flour received the same acceptability as wheat-based noodle. The cooking quality characteristics differed significantly among the noodle samples; the cooking time ranged from 6.5 to 10.3 min, it was more in wheat-based noodles than noodles from rice flour. Cooking loss; water absorption percent; cooked weight and swelling index values ranged from 0.1 to 0.6%, 137.1 to 231.6%, 23.74 to 33.24 g/g and 3.20 to 5.38 %, respectively; which revealed that a significant hindered was occurred in the functional paste properties and cooking quality parameters with increasing the level rice flour in noodles. The study indicated that, noodles prepared from mixtures of wheat and rice flour showed that the best acceptable sensory attributes and cooking quality characteristics were observed at blending ratio 40 : 60% rice flour : wheat flour . These findings enhance the utilization of non- conventional flours like rice flour for the development of value added noodles with reduced gluten content and boost nutritional value.

Facing the food industry in Egypt, the challenges of external and internal negative impact on their competitiveness and access to global markets and the study showed that the number of enterprises operating in this sector amounted to about 37.7 thousand that run around 357 thousand workers. It also represents the food exports of approximately 31.7% and 14.3% of the total value of exports of industrial goods and the College Rankings 2016, the average annual value of the products of that sector, about 21.4 million pounds, accounted for about 31% of the value of industrial production for the same year. The research problem was the small size of the internal and external markets of frozen Egyptian vegetables. Despite the increasing global demand for frozen products, Egyptian exports do not increase at the expected rate. The rate of increase in global demand for frozen food products exceeds 510% annually, Compared to its Egyptian counterparts. The study aimed at studying the internal and external markets of Egyptian frozen vegetables. The study aims at identifying the economic characteristics of the local market for frozen vegetables in general, and in particular for the field sample of vegetable freezing plants and processing them in addition to the internal markets for marketing these vegetables. Prices, categories and consumer attitudes towards them. The study was based mainly  on the available and available data published and unpublished by government agencies such as the Central Administration of Agricultural Economics in the Ministry of Agriculture and Land Reclamation, the General Organization for Industrialization, the Chamber of Food Industries in the Federation of Industries, the Commodity Council for Food Industries, the Holding Company for Food Industries, the Commercial Representation Authority, General for Export and Import Control, as well as foreign trade base data at the National Information Center of the Central Agency for Public Mobilization and Statistics. And the most important results as follows that the difference between the prices of the products, vegetables, frozen for different companies in Super accumulator different varieties where ranked Alabama to buy smile from the above parties between the varieties by about 71% of the total parties the company has a smile, and then are classified Mallow estimated at around 14.8 % of the total group has a company-Bessemer, as expected from the results I classified the grape of the biggest differences between varieties increased by approximately 26.7% of the total team purchase Montana, the following varieties of spinach increased by approximately 24.4 % of the total group company Montana.

The poultry industry in Egypt is one of the most important industries that contribute in providing the source of animal protein, which is characterized by high nutritional value, cheap price and high conversion coefficient of food compared to red meat. This industry is characterized by several characteristics, the most important speed of turnover of capital as well as not need a large agricultural patch, In addition to the relative increase in food conversion efficiency compared to other types of animals. The industry is also characterized by high return and low capital required to invest in this field compared to other productive projects. The problem of research is the high price of the prices of the inputs of the production of poultry and the low quality of the market, and fluctuation of market prices of the final output, which affects the revenue and thus affect the size of the economic project. Therefore, the study aimed at estimating the economic yield of the poultry farms in the fattening nurseries in Qalyubia governorate. These farms are characterized by a short cycle of the capital cycle in order to contribute to raising the level of individual income in the rural sector. It also provides job opportunities for young people. Of the animal protein, and based on published data and the use of field study for a sample of the municipality of Qalyubia, the research reached some results, including:  1- The average number of farms of broiler fattening was about 1.6 thousand farms by 6.67% of the total number of farms at the level of the province of Qalyubia. 2- The study of the most important problems and obstacles facing the poultry growers found that the problems of production and marketing were the high prices of chicks used in fattening, lack of quality chick, high feed prices, the lack of trained labor, the problem of control of traders and brokers in price, and the problem of irregular market price . 3- By dividing the sample of the study into three production capacities, it was found that the net yield of the first capacity (12.9-6 thousand birds) amounted to 308.9 thousand pounds / cycle, and net return of the second capacity (less than 6 thousand - more than 3.6 thousand birds) LE 323.2 thousand / cycle, while it amounted to about 274.1 thousand pounds / cycle for the third capacity (3.6 - 0.8 thousand birds). 4- The estimated profitability of the pound for the investor for the three capacities (first, second and third) was respectively 1.05, 1.12, 1.31 thousand pounds.

ABSTRACT The effect of different nitrogen sources (glucosamine sulfate, ammonium sulfate, aspartic acid, phenylalanine and peptone) in comparison to sodium nitrate, the major nitrogen compound in basal agar Czapek’s synthetic medium growth were studied on the linear growth of Rhizoctonia solani and its pathogenicity on faba bean germinated seeds. Ammonium sulfate exhibited faster liner growth and showed the same effect as the basal medium with sodium nitrate while, glucosamine sulfate showed less growth rate compared with sodium nitrate. Glucosamine sulfate and ammonium sulfate showed significant reduction in number of infection cushions which led to significant decrease of disease index in vitro. Our results showed strongly significant positive correlations between disease index and both of diameters of hyphae and number of infection cushions while, very weak correlation coefficient was found between disease index and linear growth of R. solani. Under greenhouse conditions, glucosamine sulfate or peptone as a sole nitrogen sources in food requirements of Rhizoctonia solani inoculum depressed the virulence of the fungus. The effect of different amounts of glucosamine sulfate was determined on fungal growth rate, infection cushions, disease index in vitro and polyphenol oxidase activity. Increasing amount of glucosamine sulfate showed significantly reduction of growth rate in comparison to the basal medium with sodium nitrate. All seeds subjected to R. solani grown on different amount of glucosamine sulfate showed the lower number of infection cushions, disease index and polyphenol oxidase activity compared with sodium nitrate. Under greenhouse conditions, disease index showed significantly decreased effect when glucosamine sulfate used as soil applications and showed better effect on shoot weight and root weight compared with control plants treated with sodium nitrate. Our study proposes that glucosamine sulfate may acts as controlling factor of pathogenicity genes of R. solani. Key words: Rhizoctonia solani, faba bean, Vicia faba, nitrogen sources, glucosamine sulfate, pathogenicity.

The aim of this research was to utilization of the salt whey and milk permeate to produce exopolysaccharides (EPSs). Where cheese whey is simultaneously an effluent with nutritional value and a strong organic and saline content. However, it is drained in the sewers, the EPSs are highly heterogeneous polymers produced by different species of bacteria and have recently been attracting considerable attention from biotechnologists because of their potential applications in many fields. Thus, we have isolated some halophilic bacteria that showed the ability to produce EPS from whey and milk permeate. A total of 46 strains of moderately halophilic bacteria were isolated from two types of samples. The first type was dairy samples (Baramily cheese whey and mish cheese), while the second type was water samples (salty lake water and its sediments) of Wadi El-Natrun Valley (lakes Hamra, UmRisha, and Baida), Beheira Governorate, Egypt. From isolated strains there are twelve strains were having the ability to produce exopolysaccharides but only seven strains can produce EPS from whey and milk permeate. The growth conditions i.e. concentrations of NaCl, pH value and different incubation temperature, of isolates were determined. The effect of these conditions on the production of EPS was investigated. The obtained results indicated that the optimum conditions for the production of EPS by these strains were 10 % NaCl, pH 7 and the optimum incubation temperature was 37°C. Three strains showed the highest production of exopolysaccharides. These strains were identified using two methods the first method was biology system and the second one was 16S rRNA sequence analysis method. It could be identified as Alteribacillus bidgolensis and Bacillus licheniformis. Alteribacillus bidgolensis (strain P4B) produced the highest amount of EPS (52 g/L) from whey followed by Bacillus licheniformis (DSM 13) (42 g/L), while the highest amount of EPS produced from milk permeate was (43 g/L) by Alteribacillus bidgolensis (strain P4B) followed by Bacillus licheniformis (DSM 13) (36 g/L).

The present study was suggested as an attempt to investigate the effects of Pink Pigmented Facultative Methylotrophic Bacteria (PPFM) dipping and foliar spray on vegetative growth and yield of eggplant (Solanum melongena L.). Field experiments were carried out during the summer seasons of 2014 and 2015 at the Experimental Farm, Faculty of Agriculture, Ain Shams University. The vegetative growth of eggplant responded positively to dipping seedlings in PPFM which gave the highest values of growth parameters, i.e. number of leaves per plant, leaf area, nitrogen & potassium concentration in leaf and total protein in fruit, in the two seasons as compared with control (dipping in water). The application of PPFM as foliar spray, increased significantly plant growth (plant length, leaf number, potassium concentration in leaf, vitamin "C" in fruit and total yield per plant) in the two seasons as compared with the other studied foliar application treatments. The interaction between dipping seedlings in PPFM and PPFM foliar spray with all concentrations gave synergistic effects on growth parameters and yield components of eggplant, during the two growing seasons as compared with either individual foliar application or control plants.

The experiments were conducted to evaluate the effect of mycorrhizae (Glomus sp. and Gigspora sp.) application on the vegetative growth and yield of tomato plants (Alisa cultivar), and its relevance to the mineral fertilizers requirements as NPK during the seasons of 2015/2016 and 2016/2017. The experiments were conducted at Baloza Research Station, Desert Research Center, North Sinai Governorate, Egypt. The experimental design was a randomized complete block design with three replicates, every replicate included 4 treatments (75 and100% of the recommended mineral fertilizer only or combined with the mycorrhizae treatment). The results showed that mycorrhizae application significantly increased the vegetative growth characters of tomato plant such as plant height, number of shoots, number of leaves, stem diameter, fresh and dry weights and yield. There were no significant differences in the vegetative growth characteristics of tomato plants receiving 75% of mineral fertilizers in addition to mycorrhizae application as compared with plants receiving 100% of mineral fertilizers only, except for the fresh weight of plants. The plants were received 100% of the recommended mineral fertilizers combined with mycorrhizae treatment gave the highest values of vegetative growth characters and yield. Therefore, we recommended that, using of mycorrhizae could be affective to maximize the utilization of mineral fertilizers, in addition, the mycorrhizea is ecofriendly, economically and consid ered as one of the most important bio-fertilizer resources.