The aim of this study was to optimize the production of polyunsaturated fatty acids (PUFAs), using the response surface methodology (RSM), to use the produced PUFAs in kareish cheese processing. PlackettBurman design (PBD) was employed to screen media components that affect PUFAs development (glucose, olive cake, yeast extract, tryptone, MgSO4, KH2PO4, NH4Cl, agitation speed, incubation time, and pH), and results showed that olive cake and yeast extract, with confidence level > 98%, had a positive effect on PUFAs production. The central composite design (CCD) of the response surface methodology was used to optimize the selected parameters levels where maximum PUFAs production (1790 mg/l) was observed near the mid-point (0) values (concentrations) of olive cake (15 g/l), tryptone (7.5 g/L) and KH2PO4 (1.25 g/l). Polyunsaturated fatty acids account for 47.83 % of the total fatty acid profile, according to gas chromatography analysis of the collected PUFAs. The produced PUFAs was encapsulated using whey protein concentrate and maltodextrin, freeze dried, grinded and incorporated in Kareish cheese manufacture. The average particle size of a 0.005% suspension of oil microcapsules was 671.4 nm with a poly dispersity index of 0.611 indicating a moderate stability of the emulsion. The negative zeta potential of the microcapsules particles was -37.6 mv, which is identical to the -42 mv value recorded in the literature for oil emulsions stabilized by whey protein, maltodextrin, and K-carrageenan. The addition of 0.5 % PUFAs-containing microcapsules to Kareish cheese increased antioxidative activity to 38.13 % compared to 30.14 % for the control, as well as Texture profile analysis (TPA) parameters including hardness, cohesiveness, gumminess, and chewiness. The elasticity of the Kareish cheese sample increased slightly by the addition of 0.5% microcapsules, but higher concentration tended to change the elasticity to a brittleness of the cheese structure.

The study was conducted on a knapsacksprayer powered by a DC motor through a 12 V lead acid battery charged from a solar panel (photovoltaic) directed to sun rays. The system was installed and tested in the western desert on a small-scale farm at Frafra Oasis (New val-ley). This study aimed to evaluate and develop a knapsack sprayer powered by a solar energy for overcoming operator fatigue and shortage of traditional energy in Egypt’s western desert. The experiments were conducted during the period which starts in August 2019 till Febru-ary 2020. The main parts of the system consist of photovoltaic (PV) or solar cells, charger controller, rechargeable battery, and DC motor speed control with dimmer and pump which was attached with a tank 20 liters capacity for sucking and pressurizing the liquid to the boom of the sprayer through a flexible hose. The main results of experiments were as fol-lows: The time for recharging the battery com-pletely was found 330 minutes (5.5 hours) to charge completely from 10.55 V – 13.85 V, with current intensity 1.67 Ampere. The bat-tery discharge time was varied due to drop the battery voltage from 13.85 – 10.55 Volts. De-pending on the motor speed, which is the time for discharging the battery was found 4 – 9 hours, when motor pulled 0.85 - 1.85 Amps at 2000 - 3400 rpm and this time at deferent speed is enough to spray area 3 - 6.82 fed/day "day work = 6 hours" with application rate 67 - 104.7 l/fed. The number of droplets and the area coverage percentage ranges from 30 – 146.33 drops and 13.96 – 27.97% for one cm2 of simulator targets at a speed ranging from 2000 – 3400 rpm, respectively.

Since the mid-1950s, the volatile structure of butter oil and butter were researched, and an exhaustive list of elements has been collected. Diacetyl is an aromatic popular synthetic fragrance that gives food a buttery taste used in ice cream, snacks and potting with butter, strawberry, caramel, or cheese flavor. The chromosomal aberrations and micronuclei are commonly used biomarkers of chromosomal damage, genome stability, and cancer risk assessment.In vivo trials are still important to assess the genetic toxicology of chemical products such as industrial chemicals, pharmaceuticals, and food additives. This study aimed at assessing the potential genotoxic effect of diacetyl and butter flavors on swiss albino mice using alterations in liver function enzymes, micronucleus (MN), and chromosomal aberrations (CA) assays. The results showed that exposure of swiss albino mice malesto diacetyl and butter flavors induced (CA) and (MN) in a statistically highly significant manner compared to the control. Meanwhile, the biochemical analysis revealed that these substances caused an exceptional rise in liver function enzymes (AST, ALT, and ALP) activity in serum of treated experimental animals.In conclusion, both tested compounds have increased the chromosomal aberration, micronucleus test, and serum levels of liver function enzymes indicating their high potential of being cytotoxic and genotoxic materials.

The present study was carried out during 2019 and 2020 seasons on Alphonse mango trees to evaluate the effect of some growth reg-ulators and systemic copper complexes on flo-ral malformation, yield and quality. Vegetative growth characters were superior with 200 NAA ppm and 100 ppm cofret. However, total chlorophyll, total carbohydrates and C/N ratio recorded the highest values with 200 ppm NAA. Macro elements N, P, K and Mg % were higher with GA3 at 100 ppm, whereas the highest values of micro elements Fe, Zn, Mn and Cu (ppm) were obtained with perfecto-one at 100 ppm. The number of total panicles/tree was increased with NAA 200 ppm and per-fecto-one 100 ppm treatments than other treat-ments. The number and percent of malformed panicles/tree were significantly decreased with the applied treatments than in control.. An in-creasing in total indoles, total phenols and pol-yphenol oxidase activity were recorded with 200 ppm NAA and 100 ppm perfecto-one treatments than in others. Final fruit set% and the number of fruits /tree were increased with the treatments of 200 ppm NAA and 100 ppm perfecto-one. However, the highest values of fruit weight were recorded by GA3 and perfecto-one both at100 ppm treatments, whereas the highest yield/tree values were rec-orded by NAA 200 ppm and 100 ppm perfecto-one. Finally, fruit quality involved TSS, acid-ity, L-ascorbic acid, total sugars and total ca-rotenoids were greatly improved with the ap-plied treatments than in control.

Growing wastewater microalgae contrib-utes to the elimination of nutrients present in wastewater because they need N and P for the synthesis of proteins, nucleic acid, and phos-pholipids. The most sustainable approach for achieving high biomass and high lipid build-up, along with environmental protection. In the current research, nine microalgae were culti-vated on two variations of wastewater (agricul-tural and industrial dyes) and compared to the synthetic medium. The results indicated that after three weeks of the incubation period, the ability of microalgae to grow in sterilized (syn-thetic medium and wastewater) and gave greater biomass and chlorophyll (a) than in non-sterilized ones. Out of the tested nine mi-croalgae, four microalgae (Anabaena oryzae, Spirulina platensis, Anabaena sp (2) and Nos-toc sp) were selected which gave the highest significant values of dry weight, biomass productivity and chlorophyll (a) content. The selected microalgae showed the highest signif-icant values of the chemical composition, i.e., total carbohydrate, protein and lipid when cul-tivated in agricultural wastewater more than those cultivated in both industrial dye’s wastewater and synthetic broth medium. Among four microalgae, two microalgae of A. oryzae HSSASE6 (KT277789), and S. platen-sis NIES-39 (A00800) were chosen where the chemical composition contents ranged from 1.17 to 1.21-fold and 1.03 to 1.06-fold of car-bohydrate, 1.06 to 1.09-fold and 1.88 to 1.93-fold of protein and 1.41 to 1.52-fold and 1.76 to 1.90-fold of lipid more than those of other microalgae, respectively. The agriculture wastewater was inoculated with a single cul-ture of A. oryzae HSSASE6 (KT277789) or S. platensis NIES-39 (A00800) individually with 10 % of inoculum size, which more preferred than was inoculated with consortia culture. Re-sults also showed that A. oryzae HSSASE6 (KT277789) was more efficient strain for giv-ing biomass and productivity in agricultural wastewater than S. platensis NIES-39 (A00800) (about 28% more).

Our study was carried out at the farm of Faculty of Agriculture, Ain Shams University, Egypt. The average temperatures were 18.4°C and 23.9 °C from December 2017 - February 2018 to study the effects of Saccharomycescerevisiaeboulardii supplementation (as feed additives) on productive performance of growing rabbits. Sixty growing rabbits of the NZW (New Zealand White Rabbits), (4 weeks old) and weighted (603.62 gm mean body weight) were randomly distributed into 4 treated groups, n=15 rabbits per each group. Rabbits of supplemented groups were given 100 (R1), 200 (R2) and 400 (R3) gm/ton feed of Saccharomyces cerevisiaeboulardii mixed in the diet for 6 successive weeks. Individual live body weight was noted weekly from start till the end of the experiment. At the end of the experimental period, 10 rabbits (from each group) were fasting for 12 hrs, then slaughtered for carcass traits measure. Experimental lighting system was 16-hours photoperiod /day (16L: 8D) through both daily natural and the artificial fluorescent lighting. A pelleted ration of 17.3% crude protein, 13.37% crude fiber and 2510 kcal of digestible energy per kg feed was offered ad libitum all the time. Results revealed significant (P≤0.05) increases in the final body weight and daily weight gain with saccharomyces cerevisiae supplementation when compared with control group. Though, significantly (P≤0.05) improved feed conversion ratio in all treated groups compared with control one were noticed. Feed intake was the lower in treated groups than control group. There were significant (P≤0.05) increasing in hot carcass weight and carcass percentage with respect to the control group. Supplemented Saccharomycescerevisiae reduced (P≤0.05) the liver, heart, lungs and kidneys percentages compared the control group. Supplemented rabbit feeds with saccharomycescerevisiaeboulardii in their diets had highly increasing in relative economic efficiency and clear improvement of net revenue than control group.

Fish play an essential role in food security and are regarded as one of Egypt's primary food resources and, therefore, significantly im-pact the national economy. The three main sources of fish production in Egypt: marine (Red and Mediterranean seas), lakes and the River Nile, due to the elevated incidence of species substitution at the global level, precise identification of seafood species on the mar-kets considered a solution for food safety con-trol institutions and human consumer protec-tion. Mislabeling occurs when one species is substituted for another. Different species of red sea marine fish were collected from the Egyp-tian market. According to the Arabic name in the fish market, Samples searched for English and Latin family names in (www.fishdata-base.org). Commercial samples were success-fully extracted and confirmed with 1.5% aga-rose gel electrophoresis. The result showed that the mtDNA gene cytochrome c oxidase subunit I (COI) amplification was successful for all samples with high concentration, it was purified and sequenced for both directions and blasted using the NCBI database. Resulting in the identification of three commercially essen-tial and expensive fish samples, Baghbaghan (parrotfish), Hamour (Greasy Grouper) and kahaya (Spiny squirrelfish). One of them cor-rectly identified according to the market name, while the other two are recorded mistakenly under another name.

An experimental field trial was conducted in seasons of 2015 and 2016 at Giza Experimental Station of Agricultural Research Centre (ARC), Egypt to evaluate the foliar spray effect with transpiration regulators (Potassium, Kaolin and Ascorbic acid) and three levels of irrigations (100%, 80% and 60% of Et0) on yield, its attributes, and water productivity of two sunflower cultivars (Giza 102 and Solala 120). The factorial treatments were arranged in split- split plot design with three replications. The irrigation levels (100%, 80% and 60% of Et0) were devoted for main plots, while subplots contained the three spraying treatments of transpiration regulators. (Potassium at 1%, Kaolin at 3% and Ascorbic acid at 100 ppm), in addition to control treatment (water spray). Sub-sub plots were occupied by the two sunflower cultivars (Giza 102 and Solala 120). Obtained results proved that, increasing irrigation levels up to (80- 100%) caused significant increase in all growth characteristics, photosynthetic pigments (total chlorophyll), relative water content (RWC %) and seed oil %. Also, yield parameters being head diameter, 1000-seed weight and seed yield) show significant reduction under water deficit. Foliar spray with (Potassium at 1%, Kaolin at 3% and Ascorbic acid at 100 ppm), positively affected all the growth and physiological criteria of the tested plants compared with control. Generally, under low irrigation levels, application of transpiration regulators effectively reduced the detrimental impact of drought stress on growth, yield and its components of the two used cultivars in the two seasons. While, foliar spraying of potassium under 100% of Et0 gave the best treatment in this respect. Concerning cultivars, WUE (water use efficiency) for Solala 120 was significantly higher than for Giza 102 in the two seasons. All interactions among factors under study due to WUE were not significant in 2015 and 2016 seasons.

The effect of two probiotic products with Palm Kernel Cake (PKC) as source of dietary protein and/or energy in ruminant diets on the growth performance and some blood parame-ters of Barki lamps were evaluated. Two com-mercial probiotic formulas (ZAD and ICEC-Trol) were administrated directly with theirap-plications: P1 (without probiotic), P2 (ZAD), P3 (ZAD + ICEC-Trol), the combination of four different rations: R1 (control), R2 (PKC as source of energy), R3 (PKC as source of en-ergy and protein) and R4 (PKC as source of protein). Barki lambs (n=96, 35kg average body weight) were randomly assigned to 12 experimental groups in a completely random-ized block design. (8 lambs for each group) and fed for 47 days. ZAD probiotic was ap-plied at 2 ml/head/day and ICEC-TROL was applied at 3 g/head/day. Animals weight gain and average daily gain were measured and Glucose, total protein, albumin, calcium and phosphorus were measured. Results showed that animals fed control ration (R1) with P2, R2 with P3 and R4 with P3 showed highest (P<0.05) total weight gain (10.75, 10.88 and 10.56, respectively) and average daily gain (0.229, 0.231 and 0.225, respectively). ZAD probiotic improved serum total protein, globu-lin, glucose when using with PKC in lambs’ rations. In conclusion, using PKC as a source of energy or protein, with two sources of pro-biotics in lambs’ rations showed to be more ef-fective in weight gain, while using one probi-otic (ZAD) is enough for fattening with tradi-tional rations.

Twelve local Zaraiby goats with average live body weight of 10.71±0.23 kg (5-6 months). Animals were divided into three comparable groups. Goats in first group were fed without supplement (control group) or sup-plemented with 25% ordinary cobalt and 25% nanocobalt from cobalt requirements (NRC, 1985). The results showed that no significant difference (P>0.05) among experimental groups for DMI and CPI values. However, there was a highest significant difference (P<0.05) in average daily gain, total gain, and feed efficiency (kg gain/ kg DMI) for nanoco-balt group. Nutrients digestibility values as DM, CP, CF, NFC, ADF, NDF, cellulose and hemicellulose in addition to percentage of TDN in nanocobalt treatment were higher sig-nificantly than those others groups. But the ra-tio of nitrogen balance in goats' diet with nano-cobalt group was less than that using ordinary cobalt and control group. While the DE (Mcal/Kg DMI) ratio was no significant differ-ences observed among groups. Rumen pH and TVFA’s values after 3 and 6 hours of feeding, the control and the ordinary cobalt groups in-creased (P<0.05) more than the nanocobalt group. After 3hrs, adding nanocobalt has lower significant values for NH3-N than those others. Where, the control group recorded the lowest value. No significant (P>0.05) differences were observed at 0, 3and 6 hrs. after feeding for all blood parameters. There was a slight in-crease for most of body measurements with nanocobalt supplement. It could be concluded that adding nanocobalt for goats' rations en-hanced growth performance, digestibility coef-ficients, rumen parameters and slightly body measurements without any diverse effect on animals' health.