Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubers

Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures. Despite the benefits of storage at low temperatures, it causes undesirable phenomena; one of them is cold-induced sweetening, which reduces the quality and the commercial value of the potato tubers. In this study, the CRISPR technology ″Clustered Regularly Interspaced Short Palindromic Repeats″ has been used in potato cultivar Desiree via Agrobacterium mediated transformation to edit starch phosphorylase gene that is responsible for starch degradation in cold temperature storage, after regeneration steps. The regenerated plants were used in the screening of genome editing by Sanger sequencing and the ICE program. Screening results showed that Desiree 2 (DE2), Desiree 7 (DE7) and Desiree 27 (DE27) lines have mutations in sgRNA sequence that indicated a significant reduction in reduced sugar contents of tubers after cold storage for 90 and 120 days. The reduction mean values were 29.224, 35.078 and 35.862 in DE2, DE7 and DE27 respectively after 90 days and 83.212, 56.674 and 34.109 after 120 days without visible differences in plant morphology and tubers

Potato tuber starch characteristic is influenced by the ratio of amylose to amylopectin, which is affected by the granule-bound starch synthase (GBSS) gene. GBSS gene expression was decreased in the leaves of the Desiree potato variety utilizing genome editing using CRISPR-Cas9. Constructs encoding Cas9 gene and sgRNAs targeting GBSS gene were inserted into plant leaves using Agrobacterium-mediated transformation delivery. The results obtained lines with mutations in GBSS genes accounting for 21% of regenerated shoots. The identification of mutations within one base pair of the used guide sequence provided further evidence of the considerable similarity between the target region around the protospacer adjacent motif (PAM) position and the used guide sequence. Transforming DNA into potato leaves produced mutants that lacked the Cas9 gene. Using microscopic inspection of iodine-stained starch granules, the increase of amylopectin in the starch granules of editing potato tubers was evaluated.

Potato tuber starch characteristic is influenced by the ratio of amylose to amylopectin, which is affected by the granule-bound starch synthase (GBSS) gene. GBSS gene expression was decreased in the leaves of the Desiree potato variety utilizing genome editing using CRISPR-Cas9. Constructs encoding Cas9 gene and sgRNAs targeting GBSS gene were inserted into plant leaves using Agrobacterium-mediated transformation delivery. The results obtained lines with mutations in GBSS genes accounting for 21% of regenerated shoots. The identification of mutations within one base pair of the used guide sequence provided further evidence of the considerable similarity between the target region around the protospacer adjacent motif (PAM) position and the used guide sequence. Transforming DNA into potato leaves produced mutants that lacked the Cas9 gene. Using microscopic inspection of iodine-stained starch granules, the increase of amylopectin in the starch granules of editing potato tubers was evaluated.

Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.

Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.

This study aimed to use the organic polymer, chitosan, as a flocculant for harvesting microalgae Chlorella. sp. Various polymer concentrations were tested. Within three min, 50 mg/L of chitosan showed the highest efficiency (98.9±0.5%), at pH 6.3, while the lowest efficiency (86.7±0.5%) was observed at pH 7.5. Harvesting efficiency in this study was chitosan concentration dependent. The present study showed that chitosan can be a promising and sustainable solution as an organic source and environmentally friendly flocculant. Additionally, it has excellent harvest productivity and requires minimum effort for quick outcomes.

This study aimed to use the organic polymer, chitosan, as a flocculant for harvesting microalgae Chlorella. sp. Various polymer concentrations were tested. Within three min, 50 mg/L of chitosan showed the highest efficiency (98.9±0.5%), at pH 6.3, while the lowest efficiency (86.7±0.5%) was observed at pH 7.5. Harvesting efficiency in this study was chitosan concentration dependent. The present study showed that chitosan can be a promising and sustainable solution as an organic source and environmentally friendly flocculant. Additionally, it has excellent harvest productivity and requires minimum effort for quick outcomes.

The purpose of the research was to illustrate the effects of feeding Nile tilapia fingerlings (Oreochromis niloticus) with low doses of aflatoxins (AFs) with and without vitamin C supplementation to investigate the capacity of vitamin C in detoxification. Ten experimental diets were formulated and divided into three categories. The first category included: T1, T4 and T7 feedings included 20, 40 and 80 μg AFs kg−1 feed respectively, while both the second category T2, T5 and T8 and the third category T3, T6 and T9 were treated with contaminated feed with AFs for 57days then both categories shifted to different regime till the conclusion of the experimentation. The second category was fed uncontaminated feed while the third category was fed a supplemented diet with 100 mg Kg-1 of vitamin C. For 113 days the experiment was conducted. The results showed that shifting from a contaminated diet to an uncontaminated diet (category 2) or adding vitamin C to the contaminated diet (category 3) improved the deterioration that occurred in the values of growth performance, biochemical parameters and histological disorders caused by AFs. Furthermore, the results from the control group were superior to all the treatments.

The purpose of the research was to illustrate the effects of feeding Nile tilapia fingerlings (Oreochromis niloticus) with low doses of aflatoxins (AFs) with and without vitamin C supplementation to investigate the capacity of vitamin C in detoxification. Ten experimental diets were formulated and divided into three categories. The first category included: T1, T4 and T7 feedings included 20, 40 and 80 μg AFs kg−1 feed respectively, while both the second category T2, T5 and T8 and the third category T3, T6 and T9 were treated with contaminated feed with AFs for 57days then both categories shifted to different regime till the conclusion of the experimentation. The second category was fed uncontaminated feed while the third category was fed a supplemented diet with 100 mg Kg-1 of vitamin C. For 113 days the experiment was conducted. The results showed that shifting from a contaminated diet to an uncontaminated diet (category 2) or adding vitamin C to the contaminated diet (category 3) improved the deterioration that occurred in the values of growth performance, biochemical parameters and histological disorders caused by AFs. Furthermore, the results from the control group were superior to all the treatments.

The population genetic and molecular evidence of the Arabian goat breed's phylogenetic standing (Capra hircus) is misunderstood, and little is known about genetic diversity in this species. Despite their importance in producing meat and milk, as well as their capacity to endure difficult conditions with little access to food and water, goats are the most common animal in Arab countries. We investigated the phylogenetic history and demographics of domestic goats by sequencing a hypervariable segment of the mtDNA reference region for Baladi, Demuscous, and Zaraibi breeds, as well as sequences retrieved from 16 Arab countries. Three significantly divergent lineages associated with Africa and the Middle East were identified through phylogenetic analysis of the investigated strains of Egyptian goats' mtDNA D-loop region. All Egyptian goats were allocated to lineage A, which is made up of 445 different haplotypes, including Egyptian and Arabian goats. Diversity in haplotypes and in nucleotides for each Arabian goat population was calculated separately and estimated to be 0.99835 and 0.02576, respectively. Finally, we suggest that all goat lineages descend from a single clan. The ancient world's migration and travel may have influenced goat diversity, implying that goats had multiple matrilineal forebears

The population genetic and molecular evidence of the Arabian goat breed's phylogenetic standing (Capra hircus) is misunderstood, and little is known about genetic diversity in this species. Despite their importance in producing meat and milk, as well as their capacity to endure difficult conditions with little access to food and water, goats are the most common animal in Arab countries. We investigated the phylogenetic history and demographics of domestic goats by sequencing a hypervariable segment of the mtDNA reference region for Baladi, Demuscous, and Zaraibi breeds, as well as sequences retrieved from 16 Arab countries. Three significantly divergent lineages associated with Africa and the Middle East were identified through phylogenetic analysis of the investigated strains of Egyptian goats' mtDNA D-loop region. All Egyptian goats were allocated to lineage A, which is made up of 445 different haplotypes, including Egyptian and Arabian goats. Diversity in haplotypes and in nucleotides for each Arabian goat population was calculated separately and estimated to be 0.99835 and 0.02576, respectively. Finally, we suggest that all goat lineages descend from a single clan. The ancient world's migration and travel may have influenced goat diversity, implying that goats had multiple matrilineal forebears

The research aims to evaluate the efficacy of different levels of potassium fertilizer (8 g/plant K2O) as well as the insecticides neemix, spinosad, and imidacloprid at the rates of 6 ml, 0.33 ml, and 0.33 g/L water, respectively,  for the management of brinjal fruits and shoot borer. The two-factor randomized complete block design with three replications was used. Significant minimum shoot damage was recorded in the plots treated with imidacloprid (0%), spinosad (1.67%) and neemix (5.0%) at 81 days after planting. Similarly, a significant minimum fruit infestation (2.90%) was recorded with imidacloprid, which was lower than those of spinosad (15.16%) and neemix (19.63%). The application of potassium fertilizer at a rate of 8 g/plant showed a significant reduction in shoot infestation (2.50%) 81 days after planting. The minimum percentage yield of infested fruit was reported with spinosad (1.03%), which was at par with other insecticidal treatments. Considering the negative impacts of chemical pesticides, neemix 6 ml/L water in combination with potassium fertilizer at 8 g/plant is recommended.

The research aims to evaluate the efficacy of different levels of potassium fertilizer (8 g/plant K2O) as well as the insecticides neemix, spinosad, and imidacloprid at the rates of 6 ml, 0.33 ml, and 0.33 g/L water, respectively,  for the management of brinjal fruits and shoot borer. The two-factor randomized complete block design with three replications was used. Significant minimum shoot damage was recorded in the plots treated with imidacloprid (0%), spinosad (1.67%) and neemix (5.0%) at 81 days after planting. Similarly, a significant minimum fruit infestation (2.90%) was recorded with imidacloprid, which was lower than those of spinosad (15.16%) and neemix (19.63%). The application of potassium fertilizer at a rate of 8 g/plant showed a significant reduction in shoot infestation (2.50%) 81 days after planting. The minimum percentage yield of infested fruit was reported with spinosad (1.03%), which was at par with other insecticidal treatments. Considering the negative impacts of chemical pesticides, neemix 6 ml/L water in combination with potassium fertilizer at 8 g/plant is recommended.

The green bean crop (Phaseolus vulgaris) is infested with many insects such as whiteflies (Bemisia tabaci). An investigation was carried out to study the insecticidal activity of whitefly (Bemisia tabaci), five pesticides were used, namely Thiamothoxam, Sulfoxaflor, Spirotetramat, Pymetrozine, and Flonicamid, and three commercial natural pesticides, namely Superrego, Topnine, and Bernastar. Pesticides and natural pesticides were sprayed during the two seasons of 2020 and 2021. The obtained results in terms of the reduction rate of the insects' number were as follows: The highest rate of reduction appeared in Thiamothoxam and Sulfoxaflor treatments, where it reached above 80%, while the other pesticides were in the range of 40% to 60%, and the lowest reduction rate was 34.33% and 36.00 % for Bernastar during season 2020 and 2021 respectively. When evaluating the toxicity of these pesticides and natural pesticides against honeybees (Apis mellifera), the pesticides did not reveal any toxicity to honeybees, except for Thiamothoxam, Sulfoxaflor, and Superrego The LC50 and LD50 values against honeybees were 0.2 ppm and 0.000005µg/one honeybee for Thiamothoxam, 4.5 ppm and 0.0001125 µg/one bee for Sulfoxaflor, and 0.95 ppm and 0.00002375 µg /one bee for Superego respectively.

The green bean crop (Phaseolus vulgaris) is infested with many insects such as whiteflies (Bemisia tabaci). An investigation was carried out to study the insecticidal activity of whitefly (Bemisia tabaci), five pesticides were used, namely Thiamothoxam, Sulfoxaflor, Spirotetramat, Pymetrozine, and Flonicamid, and three commercial natural pesticides, namely Superrego, Topnine, and Bernastar. Pesticides and natural pesticides were sprayed during the two seasons of 2020 and 2021. The obtained results in terms of the reduction rate of the insects' number were as follows: The highest rate of reduction appeared in Thiamothoxam and Sulfoxaflor treatments, where it reached above 80%, while the other pesticides were in the range of 40% to 60%, and the lowest reduction rate was 34.33% and 36.00 % for Bernastar during season 2020 and 2021 respectively. When evaluating the toxicity of these pesticides and natural pesticides against honeybees (Apis mellifera), the pesticides did not reveal any toxicity to honeybees, except for Thiamothoxam, Sulfoxaflor, and Superrego The LC50 and LD50 values against honeybees were 0.2 ppm and 0.000005µg/one honeybee for Thiamothoxam, 4.5 ppm and 0.0001125 µg/one bee for Sulfoxaflor, and 0.95 ppm and 0.00002375 µg /one bee for Superego respectively.