This paper is evaluating the addition of nanocobalt on dry matter, organic matter, cel-lulose and hemicellulose degradability of In-vitro gases technique. Nanocobalt as an addi-tive were used as 0, 25, 50, 75, 100 and 125% from the animal requirements on DM basis. The regular ration (1:1 concentrate: clover hay) was incubated for 24 hours. Then 24 and 48 hours of dry matter, organic matter, cellu-lose and hemicellulose were used to research the impact of adding 25 and 50% nanocobalt to some roughages (rice, bean, wheat, bagasse and peanut) straws using In-vitro disappear-ance. Results of In-vitro gas indicated that the DMD degradability values were increased (P<0.05) in 50 and 75 %, while the highest of OMD was in 25 and 50 % of nanocobalt. The nanocobalt had no effect on pH, but had a greater influence on the values of ammonia and TVF’s, as well as the degradability of cell wall components with microbial proteins. In control ration, the gas production was high, adding cobalt (100 %) and nanocobalt (25 and 50 %) than other of addition. High values of microbial protein (MP) and microbial protein efficiency (EMP) recorded for all different nanocobalt added. Nanocobalt 25% output gas of DM, NDF, ADF, cellulose and hemicellu-lose showed increase (P<0.05) compared to any additional levels. It concluded that the ad-dition of nanocobalt increased the production of gas, ammonia, TVF’s, metabolizable energy and degradability of cell wall constituents. It showed that the addition of nanocobalt had a stronger effect on disappearance of all roughages as DM, OM, NDF, ADF, cellulose and hemicellulose. Furthermore, the addition of 25% nanocobalt had a significant effect (P<0.05) on improving In-vitro DM and OM disappearance of all roughages except bagasse had a significant effect in add 50 % nanoco-balt.

Since the mid-1950s, the volatile structure of butter oil and butter were researched, and an exhaustive list of elements has been collected. Diacetyl is an aromatic popular synthetic fragrance that gives food a buttery taste used in ice cream, snacks and potting with butter, strawberry, caramel, or cheese flavor. The chromosomal aberrations and micronuclei are commonly used biomarkers of chromosomal damage, genome stability, and cancer risk assessment.In vivo trials are still important to assess the genetic toxicology of chemical products such as industrial chemicals, pharmaceuticals, and food additives. This study aimed at assessing the potential genotoxic effect of diacetyl and butter flavors on swiss albino mice using alterations in liver function enzymes, micronucleus (MN), and chromosomal aberrations (CA) assays. The results showed that exposure of swiss albino mice malesto diacetyl and butter flavors induced (CA) and (MN) in a statistically highly significant manner compared to the control. Meanwhile, the biochemical analysis revealed that these substances caused an exceptional rise in liver function enzymes (AST, ALT, and ALP) activity in serum of treated experimental animals.In conclusion, both tested compounds have increased the chromosomal aberration, micronucleus test, and serum levels of liver function enzymes indicating their high potential of being cytotoxic and genotoxic materials.

Repellency of the six essential oils (EOs), Clove (Eugenia aromaticum), Aloe (Aloe vera), Lemon (Citrus limon), Camphor (Euca-lyptus camaldulensis), Mint (Mentha spicata), Marjoram (Origanum vulgare) were evaluated against adult of the confused flour beetle Tri-bolium confusum, by allowing adults to choose between untreated filter paper halves with 1 -2g white flour or treated with different concen-trations of the oils and their mixture at three different exposure times. In individual oils ex-periments, Eucalyptus camaldulensis was found to be the best effective of repellent effi-cacy, followed by Mentha spicata and Eugenia aromaticum. Citrus limon and Aloe vera have moderate repellent efficacy. Results of combi-nation effective showed that mixtures' repel-lant activity. It has been shown that the most powerful mixture of Clove + Mint repels activ-ity against adults with Lemon + Aloe, Lemon + Marjoram, Lemon + Mint, and Mint + Aloe during the entire exposure period, while Lemon + Camphor and Camphor + Mint lost repellent activity after 24 hours.

Two types of essential oils (thyme and Stachys) were used in this Estimation. The chemical composition, antioxidant activity (by DPPH assay) and the rancimat method were used to assess the stability effect of these essential oils. The tested oils were added to mayonnaise to evaluate its capability for inhibition of secondary oxidation products. By DPPH assay, antioxidant activity showed that thyme essential oil was the best antioxidant agent as radical scavenging agents at 450 and 500 ppm concentrations, even better than BHT at the200 ppm concentration. As a typical antioxidant, butylated hydroxytoluene was used for comparison. The mayonnaise samples prepared by adding thyme, stachys essential oils and TBHQ (control +) at concentrations of 200,400, 600 and 200 ppm into the sunflower oil free from antioxidant. At the amounts used in this study, thyme and stachys oils were significantly successful in reducing the oxidation of sunflower oil, especially, at 500 ppmwhich showed more efficient than BHT. Specifically, in the presence of thyme oil, the induction period of sunflower oil was considerably elongated. However, butylated hydroxytoluene (BHT) was more efficient than thyme and Stachys oils at the 200-ppm concentration against oxidation of oils. all concentrations of thyme and stachys essential oils had an antioxidant activity similar to synthetic antioxidant of TBHQ at a concentration of 200 ppm for inhibition of secondary oxidation products in mayonnaise in comparison with the control sample. As a conveniently available source of natural antioxidants, thyme and stachys essential oils can be used for use in fats, oils and food containing fats.

Fish play an essential role in food security and are regarded as one of Egypt's primary food resources and, therefore, significantly im-pact the national economy. The three main sources of fish production in Egypt: marine (Red and Mediterranean seas), lakes and the River Nile, due to the elevated incidence of species substitution at the global level, precise identification of seafood species on the mar-kets considered a solution for food safety con-trol institutions and human consumer protec-tion. Mislabeling occurs when one species is substituted for another. Different species of red sea marine fish were collected from the Egyp-tian market. According to the Arabic name in the fish market, Samples searched for English and Latin family names in (www.fishdata-base.org). Commercial samples were success-fully extracted and confirmed with 1.5% aga-rose gel electrophoresis. The result showed that the mtDNA gene cytochrome c oxidase subunit I (COI) amplification was successful for all samples with high concentration, it was purified and sequenced for both directions and blasted using the NCBI database. Resulting in the identification of three commercially essen-tial and expensive fish samples, Baghbaghan (parrotfish), Hamour (Greasy Grouper) and kahaya (Spiny squirrelfish). One of them cor-rectly identified according to the market name, while the other two are recorded mistakenly under another name.

In this work, electromagnetic field technol-ogy and electrolysis technology with alumi-num electrodes coated with titanate nano-coat-ing were combined in different investigated parameters: power types (Electrolysis pass (EP) and Electromagnetic pass (EMP)), volt-age (12v, 24v and 36v) and nano-coating pa-rameters (coated and uncoated). The designed water treatment unit was evaluated by record-ing the EC-meter readings for 60min (each 5min) at a flow rate of 5l/min. Results show that the best salt rejection rate was 3.4% when EMP was used before EP where four elec-trodes were coated. In contrast, the other pa-rameters (EP, EMP+EP and EMP+EP (2 coated electrodes and 2 uncoated)) recorded the salt rejection rates 1.13%, 1.57% and 2.4%, respectively. This study is promising and opens the way for integrating both electrolysis and electromagnetic field technologies with on-farm irrigation systems and enhancing their efficiency using nanotechnology.

Nanotechnology takes a significant position in controlling pests. Silicon dioxide nanoparticles (SiO2-NPs) is considered one of the best alter-natives to chemical pesticides for plant crop protection from harmful arthro-pods such as phytophagous mites, Tetranychus urticae. Therefore, this study aims to evaluate the effect of (SiO2-NPs) to control T. urticae. Under green-house conditions, the results revealed, in the larval stage, a high mortality percentage (99.05±0.83%) followed by eggs (98.74±1.22%). Besides, the nymph stage recorded high mortality (94.66±1.81%) at a high concentration of 1500 ppm. While the adult females of T. urticae recorded mortality of 91.92±1.02% after 72h. Eventually, the immature stages of T. urticae were susceptible to (SiO2-NPs). Meanwhile, at laboratory evaluation on immature stages of T. urticae which resulted from live treated females during 96h showed a sharp decrease in average numbers of eggs through 96h, at 1000 and 1500 ppm. No numbers of the larval stage were recorded at 1500 ppm. No number of nymph stage were recorded at all concentrations and periods. Moreover, activity of AChE was significantly inhibited at 1500 ppm, while activity of glutathione-S-transferase was significantly increased after 72 h. Finally, (SiO2-NPs) are effective against immature stages of T. urticae under greenhouse and laboratory conditions.

The foot and mouth disease (FMD) vaccine is used to control the FMD in cloven-hoofed animals including calves. The most important step in vaccine preparation is the selection of a suitable adjuvant which plays a main role in the vaccine’s potency. The present study is an attempt to use clinoptilolite instead of Montanide oil to enhance the efficiency of the FMD vaccine and reduce its production cost. Twelve calves were split into four groups. Three groups were vaccinated with FMD vaccine with different adjuvants i.e. 100% Montanide (ISA 206), 50% of Montanide with 1.0 μg/dose of clinoptilolite (ISA 206+CLINP), and by replacing the 100% of Montanide to 1μg/dose of clinoptilolite (CLINP). The fourth group was unvaccinated. Results showed that calves vaccinated with (ISA 206+CLINP) FMD vaccine expressed the highest and the longest periods of immune response until 38 weeks. While groups vaccinated with (CLINP) or (ISA 206) vaccine showed only protection up to 26 and 34 weeks respectively. Also, our results showed that clinoptilolite improved organ functions resulting from the stress of vaccines. Finally, this study recommended the use of clinoptilolite with Montanide oil as a potential adjuvant in the FMD vaccine to give high stimulation of the immune response.

The objectives of the current study were to investigate the opportunity of estimating soil salinity from hyperspectral data and identifying the most informative spectral zones for estimation. Electrical conductivity (EC) measurements of ninety topsoil samples (0–30 cm) collected from Toshka, Egypt, were used as data set. Analytical spectral device was employed to collect the reflectance spectral signatures of soil samples. Both linear regression and HSD Tukey’s analyses displayed that the SWIR1 and SWIR2 zones are the most suitable for soil salinity prediction while, blue, green and NIR were the wickedest. Moreover, EC estimation was better in case of lower soil salinity (0-2 dS m-1) than higher levels (8-1). Partial-least-squares-regression (ΡLSR) was employed to establish soil salinity prediction model using the training set of soil samples (n=75). The PLSR model was set up using the most informative wave bands (SWIR1 and SWIR2). The result showed that PLSR linear model gave a precise prediction of soil salinity (R2 = 0.93). The results revealed that employing reflectance values in SWIR in the model variables increases the precision of soil EC prediction.

Excessive exposure to lead may damage the liver by increasing oxidative stress, leading to many serious diseases. Dietary antioxidants may protect the liver from damage. Therefore, the effect of ethanolic and aqueous turmeric extracts against lead-induced liver damage was evaluated by estimating liver function and antioxidant/oxidative stress biomarkers. Forty rats were divided into group (A) served as the control and group (B) was treated with 93 mg/kg b.wt. lead nitrate; while group (C) and group (D) were treated as group B in addition to 400 mg/kg b.wt. of turmeric aqueous and ethanolic extracts, respectively. Group B showed a significant increase in the activities of alanine transaminase by 10 folds, aspartate transaminase by 9 folds, alkaline phosphatase by 3 folds and the level of malondialdehyde by 10 folds, while showed a significant decrease in albumin by 60%, total protein by 54.5%, and superoxide dismutase by 80.5%, catalase by 75% and glutathione by 71.6% in comparison with those of the control group. On the other hand, turmeric treatments (groups C and D) led to a readjustment of levels for such biomarkers. Collectively, these results demonstrate the potential of turmeric extracts (particularly the ethanolic extract) to improve the fatal effect of lead in a rat model.